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Regulatory Pattern of PUN Promoter for Gene Expression

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Date Issued:
2016
Summary:
The purpose of this research was to analyze the regulatory pattern of the PUN promoter in the expression of a marker gene, β-glucoronidase (GUS), within regenerated tobacco plants. The genes for neomycin phosphotransferase (NPT II) and GUS were included in the coding region of the Ti plasmid construct. The NPTII gene drove antibiotic resistance and was used to select and identify homozygous lines through the segregation of the progeny. Analysis through histochemical staining and genetic assays rendered putative transgenic lines that were cultivated for further assessment of progeny. First generation histochemical analysis of 14-day tissue formation resulted in no levels of expression for the GUS gene, which demonstrated that the flower-specific PUN promoter was not active in the leaf tissue. Further testing of gene activity throughout all stages of tissue formation for the first generation lines is required in order to assess regulatory pattern of the PUN promoter.
Title: Regulatory Pattern of PUN Promoter for Gene Expression.
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Name(s): Velez, Stephanie
Kirke, Justin
Zhang, Xing-Hai
Office of Undergraduate Research and Inquiry
Type of Resource: text
Genre: Poster
Date Created: 2016
Date Issued: 2016
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Florida
Physical Form: application/pdf
Extent: 1 p.
Language(s): English
Summary: The purpose of this research was to analyze the regulatory pattern of the PUN promoter in the expression of a marker gene, β-glucoronidase (GUS), within regenerated tobacco plants. The genes for neomycin phosphotransferase (NPT II) and GUS were included in the coding region of the Ti plasmid construct. The NPTII gene drove antibiotic resistance and was used to select and identify homozygous lines through the segregation of the progeny. Analysis through histochemical staining and genetic assays rendered putative transgenic lines that were cultivated for further assessment of progeny. First generation histochemical analysis of 14-day tissue formation resulted in no levels of expression for the GUS gene, which demonstrated that the flower-specific PUN promoter was not active in the leaf tissue. Further testing of gene activity throughout all stages of tissue formation for the first generation lines is required in order to assess regulatory pattern of the PUN promoter.
Identifier: FA00005606 (IID)
Subject(s): College students --Research --United States.
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00005606
Restrictions on Access: Author retains rights.
Host Institution: FAU