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Creation of a bacteria surrogate to accelerate research on Ebolavirus Zaire

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Date Issued:
2016
Summary:
Ebola Viral Disease (EVD) is a devastating illness with high infectivity and mortality rates. The 2014 West African EVD outbreak was unprecedented in case numbers and fatalities, and has highlighted the need to develop rapid Point of Care detection devices. Progress in the diagnosis and treatment of highly virulent pathogens like the Ebola virus is often limited by the small number of labs adequately equipped to handle them. This study is one of the first to aim at developing a non-pathogenic bacterium surrogate, containing a stable EBV gene for subsequent detection studies. Our approach entailed the use of synthetic biology, to design a recombinant vector containing the Ebola virus glycoprotein (GP) gene. The synthetic gene was spliced into a E.coli pUC19 plasmid vector by ligation and subsequently transformed into competent E. coli by cloning techniques. This Ebola virus surrogate will assist in further Ebola diagnostic platform design and testing.
Title: Creation of a bacteria surrogate to accelerate research on Ebolavirus Zaire.
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Name(s): Varghese, Reen
Holmes, Douglas
Coarsey, C.
Singkorat, D.
Office of Undergraduate Research and Inquiry
Type of Resource: text
Genre: Poster
Date Created: 2016
Date Issued: 2016
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Florida
Physical Form: application/pdf
Extent: 1 p.
Language(s): English
Summary: Ebola Viral Disease (EVD) is a devastating illness with high infectivity and mortality rates. The 2014 West African EVD outbreak was unprecedented in case numbers and fatalities, and has highlighted the need to develop rapid Point of Care detection devices. Progress in the diagnosis and treatment of highly virulent pathogens like the Ebola virus is often limited by the small number of labs adequately equipped to handle them. This study is one of the first to aim at developing a non-pathogenic bacterium surrogate, containing a stable EBV gene for subsequent detection studies. Our approach entailed the use of synthetic biology, to design a recombinant vector containing the Ebola virus glycoprotein (GP) gene. The synthetic gene was spliced into a E.coli pUC19 plasmid vector by ligation and subsequently transformed into competent E. coli by cloning techniques. This Ebola virus surrogate will assist in further Ebola diagnostic platform design and testing.
Identifier: FA00005605 (IID)
Subject(s): College students --Research --United States.
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00005605
Restrictions on Access: Author retains rights.
Host Institution: FAU