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comprehensive study of mammalian SNAG transcription family members
| Title: | A comprehensive study of mammalian SNAG transcription family members. |
 126 views 53 downloads |
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| Name(s): |
Chiang, Cindy Chung-Yue. Charles E. Schmidt College of Science Department of Biological Sciences |
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| Type of Resource: | text | |
| Genre: | Electronic Thesis Or Dissertation | |
| Issuance: | monographic | |
| Date Issued: | 2012 | |
| Publisher: | Florida Atlantic University | |
| Physical Form: | electronic | |
| Extent: | xiv, 183 p. : ill. (some col.) | |
| Language(s): | English | |
| Summary: | Transcriptional regulation by the family of SNAG (Snail/Gfi-1) zinc fingers has been shown to play a role in various developmental states and diseases. These transcriptional repressors have function in both DNA- and protein-binding, allowing for multiple interactions by a single family member. This work aims to characterize the SNAG members Slug, Smuc, Snail, Scratch, Gfi-1, Gfi-1B, and IA-1 in terms of both DNA-protein and protein-protein interactions. The specific DNA sequences to which the zinc finger regions bind were determined for each member, and a general consensus of TGCACCTGTCCGA, was developed for four of the members. Via these studies, we also reveal thebinding affinities of E-box (CANNTG) sequences to the members, since this core is found for multiple members' binding sites. Additionally, protein-protein interactions of SNAG members to other biological molecules were investigated. The Slug domain and Scratch domain have unknown function, yet through yeast two-hybrid screening, we were able to determine protein interaction partners for them as well as for other full length SNAG members. These protein-interacting partners have suggested function as corepressors during transcriptional repression. The comprehensive information determined from these studies allow for a better understanding of the functional relationship between SNAG-ZFPs and other genes. The collected data not only creates a new profile for each member investigated, but it also allows for further studies to be initiated from the results. | |
| Identifier: | 794107607 (oclc), 3342041 (digitool), FADT3342041 (IID), fau:3845 (fedora) | |
| Note(s): |
by Cindy Chiang. Thesis (Ph.D.)--Florida Atlantic University, 2012. Includes bibliography. Electronic reproduction. Boca Raton, Fla., 2012. Mode of access: World Wide Web. |
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| Subject(s): |
Cellular signal transduction Zinc-finger proteins -- Synthesis Metalloproteins -- Synthesis Genetic transcription -- Regulation |
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| Held by: | FBoU FAUER | |
| Persistent Link to This Record: | http://purl.flvc.org/fcla/dt/3342041 | |
| Use and Reproduction: | http://rightsstatements.org/vocab/InC/1.0/ | |
| Host Institution: | FAU |
