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Glial Hsp70 Protects K+ Homeostasis in the Drosophila Brain during Repetitive Anoxic Depolarization

Title: Glial Hsp70 Protects K+ Homeostasis in the Drosophila Brain during Repetitive Anoxic Depolarization.
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Name(s): Armstrong, Gary A. B., author
Xiao, Chengfeng, author
Krill, Jennifer L., author
Seroude, Laurent, author
Dawson-Scully, Ken, author
Robertson, R. Meldrum, author
Roman, Gregg, editor
Type of Resource: text
Genre: Article
Date Issued: 2011-12-12
Summary: Neural tissue is particularly vulnerable to metabolic stress and loss of ion homeostasis. Repetitive stress generally leads to more permanent dysfunction but the mechanisms underlying this progression are poorly understood. We investigated the effects of energetic compromise in Drosophila by targeting the Na+/K+-ATPase. Acute ouabain treatment of intact flies resulted in subsequent repetitive comas that led to death and were associated with transient loss of K+ homeostasis in the brain. Heat shock pre-conditioned flies were resistant to ouabain treatment. To control the timing of repeated loss of ion homeostasis we subjected flies to repetitive anoxia while recording extracellular [K+] in the brain. We show that targeted expression of the chaperone protein Hsp70 in glial cells delays a permanent loss of ion homeostasis associated with repetitive anoxic stress and suggest that this is a useful model for investigating molecular mechanisms of neuroprotection.
Identifier: 10.1371/journal.pone.0028994 (doi), http://dx.plos.org/10.1371/journal.pone.0028994 (uri), FAUIR000078 (IID)
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FAUIR000078
Use and Reproduction: publisher
Host Institution: FAU
Is Part Of: PLoS ONE.
1932-6203