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Molecular Characterization of a Nicotiana tabacum Chloroplast Mutant and in vitro Regeneration of a Cattail, Typha domingensis, from Embryonic Callus

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Date Issued:
2007
Summary:
The thesis consists of two chapters, and within each chapter is a different set of methods and techniques that will be useful to me in future research endeavors. The first is the transformation of tobacco chloroplast and the analysis of a resultant chloroplast mutant, and the second is the establishment of a regeneration system for the aquatic plant Typha domingensis, commonly known as cattail. The unifying theme is plant transformation. The establishment of a regeneration system for a potentially beneficial plant is useful for future transformations and the actual transformation and analysis of mutants is useful for the characterization of transformants. The chloroplast transformation was unsuccessful and analysis of the mutation demonstrated it as a null mutation under normal growth conditions. Cattail seeds were induced to form calli, then induced to regenerate into normal plants.
Title: Molecular Characterization of a Nicotiana tabacum Chloroplast Mutant and in vitro Regeneration of a Cattail, Typha domingensis, from Embryonic Callus.
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Name(s): Tapia, Claudius E.
Zhang, Xing-Hai, Thesis advisor
Florida Atlantic University, Degree grantor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Date Created: 2007
Date Issued: 2007
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 44 p.
Language(s): English
Summary: The thesis consists of two chapters, and within each chapter is a different set of methods and techniques that will be useful to me in future research endeavors. The first is the transformation of tobacco chloroplast and the analysis of a resultant chloroplast mutant, and the second is the establishment of a regeneration system for the aquatic plant Typha domingensis, commonly known as cattail. The unifying theme is plant transformation. The establishment of a regeneration system for a potentially beneficial plant is useful for future transformations and the actual transformation and analysis of mutants is useful for the characterization of transformants. The chloroplast transformation was unsuccessful and analysis of the mutation demonstrated it as a null mutation under normal growth conditions. Cattail seeds were induced to form calli, then induced to regenerate into normal plants.
Identifier: FA00000839 (IID)
Collection: FAU Electronic Theses and Dissertations Collection
Note(s): Includes bibliography.
Thesis (M.S.)--Florida Atlantic University, 2007.
Charles E. Schmidt College of Science
Subject(s): Plant cell culture
Plant biotechnology
Typha
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00000839
Sublocation: Digital Library
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
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Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.