You are here
Co-Cultures of Pseudomonas aeruginosa and Roseobacter denitrificans Reveal Shifts in Gene Expression Levels Compared to Solo Cultures
| Title: | Co-Cultures of Pseudomonas aeruginosa and Roseobacter denitrificans Reveal Shifts in Gene Expression Levels Compared to Solo Cultures. |
 81 views 20 downloads |
|---|---|---|
| Name(s): |
Conway, Crystal A., author Esiobu, Nwadiuto, author Lopez, Jose V., author |
|
| Type of Resource: | text | |
| Genre: | Article | |
| Date Issued: | 2012 | |
| Summary: | Consistent biosynthesis of desired secondary metabolites (SMs) from pure microbial cultures is often unreliable. In a proof-ofprinciple study to induce SM gene expression and production, we describe mixed “co-culturing” conditions and monitoring of messages via quantitative real-time PCR (qPCR). Gene expression of model bacterial strains (Pseudomonas aeruginosa PAO1 and Roseobacter denitrificans Och114) was analyzed in pure solo and mixed cocultures to infer the effects of interspecies interactions on gene expression in vitro, Two P. aeruginosa genes (PhzH coding for portions of the phenazine antibiotic pathway leading to pyocyanin (PCN) and the RhdA gene for thiosulfate: cyanide sulfurtransferase (Rhodanese)) and two R. denitrificans genes (BetaLact formetallo-beta-lactamase and the DMSP gene for dimethylpropiothetin dethiomethylase) were assessed for differential expression. Results showed that R. denitrificans DMSP and BetaLact gene expression became elevated in a mixed culture. In contrast, P. aeruginosa co-cultures with R. denitrificans or a third species did not increase target gene expression above control levels. This paper provides insight for better control of target SM gene expression in vitro and bypass complex genetic engineering manipulations. | |
| Identifier: | 10.1100/2012/120108 (doi), http://www.hindawi.com/journals/tswj/2012/120108/ (uri), FAUIR000016 (IID) | |
| Persistent Link to This Record: | http://purl.flvc.org/fau/fd/FAUIR000016 | |
| Use and Reproduction: | publisher | |
| Host Institution: | FAU | |
| Is Part Of: |
The Scientific World Journal. 1537-744X |
