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Elucidation of Microbial Communities From South Florida Beaches Using 16S rRNA Sequence Analysis

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Date Issued:
2007
Summary:
The composition of marine bacterial communities from South Florida beaches were characterized using 16S rRNA sequence analysis. To compare cultivable and noncultured populations, community genomic DNA was extracted directly from sand and seawater samples and from two cultured equivalents. Only two ofthe 86 (2.3%) direct extracted sequences and 79 of 150 (52.6%) culture sequences belong to known isolates in Ribosomal Database (version 9.0) at 95% confidence level. At low stringency (p=0.70), the populations cluster into several unknown clads with early divergence, indicating the presence of novel well established bacterial groups. Members of phylum Bacteroidetes, Firmicuites and Proteobacteria were identified, with the latter being the most prevalent in culture. Diversity indices rank Hollywood beach > Fort Lauderdale > Hobie beach. Taxonomic representation indicates marine water as more diverse compared to dry sand and wet sand. A combination of phylogenetic markers will be needed to define the immense diversity of this niche.
Title: Elucidation of Microbial Communities From South Florida Beaches Using 16S rRNA Sequence Analysis.
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Name(s): Ponukumati, Sushma
Esiobu, Nwadiuto, Thesis advisor
Florida Atlantic University, Degree grantor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Date Created: 2007
Date Issued: 2007
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 189 p.
Language(s): English
Summary: The composition of marine bacterial communities from South Florida beaches were characterized using 16S rRNA sequence analysis. To compare cultivable and noncultured populations, community genomic DNA was extracted directly from sand and seawater samples and from two cultured equivalents. Only two ofthe 86 (2.3%) direct extracted sequences and 79 of 150 (52.6%) culture sequences belong to known isolates in Ribosomal Database (version 9.0) at 95% confidence level. At low stringency (p=0.70), the populations cluster into several unknown clads with early divergence, indicating the presence of novel well established bacterial groups. Members of phylum Bacteroidetes, Firmicuites and Proteobacteria were identified, with the latter being the most prevalent in culture. Diversity indices rank Hollywood beach > Fort Lauderdale > Hobie beach. Taxonomic representation indicates marine water as more diverse compared to dry sand and wet sand. A combination of phylogenetic markers will be needed to define the immense diversity of this niche.
Identifier: FA00000809 (IID)
Degree granted: Thesis (M.S.)--Florida Atlantic University, 2007.
Note(s): Charles E. Schmidt College of Science
Subject(s): Marine microbiology--Florida
Evolutionary genetics
Biodiversity--Research
Marine biodiversity conservation
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00000809
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.