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Immunoassay test strip for Microcystin-LR detection
| Title: | Immunoassay test strip for Microcystin-LR detection. |
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| Name(s): |
Xu, Jiesi. Charles E. Schmidt College of Science Department of Chemistry and Biochemistry |
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| Type of Resource: | text | |
| Genre: | Electronic Thesis Or Dissertation | |
| Date Issued: | 2010 | |
| Publisher: | Florida Atlantic University | |
| Physical Form: | electronic | |
| Extent: | ix, 53 p. : ill. (some col.) | |
| Language(s): | English | |
| Summary: | Microcystin-LR (MCLR) is hepatotoxic to animals and humans with disruption of liver structure causing cytoskeletal damage, necrosis and pooling of blood in the liver, leading to large increase in liver weight. It is also a strong liver tumor promoter and protein phosphatase inhibitor. Microcysin-LR binds protein phosphatases 1 and 2A, and influences regulation of cellular protein phosphorylation. In the present study, a colloidal gold based immunoassay test strip was developed for Microcystin-LR detection. The detection limit was found to be 1 ng/mL. 5 nm colloidal gold test strips exhibits more efficient for detection, compared with 20 nm colloidal gold test strips. The interaction between Microcystin-LR antibody (immunoglobulin G) and colloidal gold nanoparticles was investigated by various analytical methods, including Ultraviolet/Visible (UV/VIS), Fourier Transform Infrared (FTIR) and Fluorescence spectroscopy as well as transmission electron microscopy (TEM). | |
| Identifier: | 651971675 (oclc), 2683532 (digitool), FADT2683532 (IID), fau:3506 (fedora) | |
| Note(s): |
by Jiesi Xu. Thesis (M.S.)--Florida Atlantic University, 2010. Includes bibliography. Electronic reproduction. Boca Raton, Fla., 2010. Mode of access: World Wide Web. |
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| Subject(s): |
Immunoassay Biosensors Environmental chemistry Cyanobacterial toxins Drinking water -- Microbiology |
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| Persistent Link to This Record: | http://purl.flvc.org/FAU/2683532 | |
| Use and Reproduction: | http://rightsstatements.org/vocab/InC/1.0/ | |
| Host Institution: | FAU |
