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Role of Methionine Sulfoxide Reductase (MsrA) on Aging and Oxidative Stress in Drosophila
- Date Issued:
- 2006
- Summary:
- Oxidative damage is an inevitable consequence of aerobic respiration. Methionine sulfoxide reductases (Msr) are a group of enzymes that function to repair oxidized methionine residues in both free methionine and methionine in proteins. MsrA was the first of these enzymes to be discovered and is the most thoroughly studied. It is thought to play a role in both the aging process and probably several neurodegenerative diseases. I recently obtained a strain of Drosophila that was reported to have a P-element transposon located within Exon 2 (part of the open reading frame) of the eip71cd gene, which is the Drosophila homolog of MsrA. Thus, the transposon insertion should disrupt expression of the msrA gene. I did a series of experiments to "jump out" the P-element in an effort to recover two types of isogenic strains. The first would be a null mutation of the MsrA gene created by deletion of flanking genomic DNA when the P-element excised from the chromosome. The second would be a precise excision of the P-element, which would restore the genetic locus to its original structure. This study looks at the effect of a null mutant of the MsrA gene on aging and resistance to oxidative stress.
Title: | Role of Methionine Sulfoxide Reductase (MsrA) on Aging and Oxidative Stress in Drosophila. |
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Name(s): |
Foss, Katie, author Binninger, David, Thesis advisor Florida Atlantic University, Degree grantor |
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Type of Resource: | text | |
Genre: | Electronic Thesis Or Dissertation | |
Date Created: | 2006 | |
Date Issued: | 2006 | |
Publisher: | Florida Atlantic University | |
Place of Publication: | Boca Raton, Fla. | |
Physical Form: | application/pdf | |
Extent: | 84 p. | |
Language(s): | English | |
Summary: | Oxidative damage is an inevitable consequence of aerobic respiration. Methionine sulfoxide reductases (Msr) are a group of enzymes that function to repair oxidized methionine residues in both free methionine and methionine in proteins. MsrA was the first of these enzymes to be discovered and is the most thoroughly studied. It is thought to play a role in both the aging process and probably several neurodegenerative diseases. I recently obtained a strain of Drosophila that was reported to have a P-element transposon located within Exon 2 (part of the open reading frame) of the eip71cd gene, which is the Drosophila homolog of MsrA. Thus, the transposon insertion should disrupt expression of the msrA gene. I did a series of experiments to "jump out" the P-element in an effort to recover two types of isogenic strains. The first would be a null mutation of the MsrA gene created by deletion of flanking genomic DNA when the P-element excised from the chromosome. The second would be a precise excision of the P-element, which would restore the genetic locus to its original structure. This study looks at the effect of a null mutant of the MsrA gene on aging and resistance to oxidative stress. | |
Identifier: | FA00000772 (IID) | |
Note(s): | Thesis (M.S.)--Florida Atlantic University, 2006. | |
Subject(s): |
Genetic regulation Oxidation-reduction reaction Antioxidants Oxygen--Physiological effect Proteins--Chemical modification |
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Held by: | Florida Atlantic University Libraries | |
Sublocation: | Digital Library | |
Persistent Link to This Record: | http://purl.flvc.org/fau/fd/FA00000772 | |
Restrictions on Access: | All rights reserved by the source institution | |
Restrictions on Access: | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
Restrictions on Access: | http://rightsstatements.org/vocab/InC/1.0/ | |
Host Institution: | FAU | |
Is Part of Series: | Florida Atlantic University Digital Library Collections. |