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Microbial community analysis of the octocoral Eunice a fusca; combined use of cultivation-dependent and independent techniques

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Date Issued:
2007
Summary:
The octocoral Eunicea fusca is the source of important anti-inflammatory compounds such as the diterpenes fuscol and the fuscosides. Evidence suggests that these compounds are being produce by bacterial sources that live in symbiosis with the coral. As part of an investigation to better understand the role of bacteria associated with E. fusca , the characterization of the bacterial community using two different techniques (culture dependent technique and culture independent technique) and sea water samples was done. Sea water samples were used as a control to determine how closely associated bacteria are to this octocoral. The polymerase chain reaction (PCR) with universal bacterial primers FC27 and RC 1492 specific to prokaryotic 16S rDNA gene sequences was used to characterize the total bacterial population when using both the culturable and the non-culturable approaches. The results indicate a diverse group of bacteria associated to E. fusca composed of 10 different groups. The pro teo bacteria group was the most predominant group when both techniques were used. The a-proteobacteria represented the highest percentage of bacteria associated to E. fusca.
Title: Microbial community analysis of the octocoral Eunice a fusca; combined use of cultivation-dependent and independent techniques.
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Name(s): Duque-Alarcon, Angela Patricia, author
Kerr, Russell G., Thesis advisor
Florida Atlantic University, Degree grantor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Date Created: 2007
Date Issued: 2007
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 93 p.
Language(s): English
Summary: The octocoral Eunicea fusca is the source of important anti-inflammatory compounds such as the diterpenes fuscol and the fuscosides. Evidence suggests that these compounds are being produce by bacterial sources that live in symbiosis with the coral. As part of an investigation to better understand the role of bacteria associated with E. fusca , the characterization of the bacterial community using two different techniques (culture dependent technique and culture independent technique) and sea water samples was done. Sea water samples were used as a control to determine how closely associated bacteria are to this octocoral. The polymerase chain reaction (PCR) with universal bacterial primers FC27 and RC 1492 specific to prokaryotic 16S rDNA gene sequences was used to characterize the total bacterial population when using both the culturable and the non-culturable approaches. The results indicate a diverse group of bacteria associated to E. fusca composed of 10 different groups. The pro teo bacteria group was the most predominant group when both techniques were used. The a-proteobacteria represented the highest percentage of bacteria associated to E. fusca.
Identifier: FA00000748 (IID)
Note(s): Thesis (M.S.)--Florida Atlantic University, 2007.
Subject(s): Diterpenes--Synthesis
Anti-inflammatory agents
Marine pharmacology
Biodiversity
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00000748
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Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.