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Characterization of Methionine Sulfoxide Reductases A and Bs from Tobacco Plant

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Date Issued:
2006
Summary:
One methionine sulfoxide reductase A (TMSRA) and two methionine sulfoxide reductase Bs (TMSRB 1 and TMSRB2) were isolated from tobacco plants. TMSRA showed specificity for the reduction of Met-(S)-SO and both TMSRBs were specific for the reduction of Met-(R)-SO. TMSRA was the cytosolic form and both TMSRBs were plastid forms based on sequence comparison and expression tests. TMSRA and TMSRB2 could use either thioredoxin (TRX) or dithiothreitol (DTT) as reducing system, while TMSRB 1 showed little activity with TRX but much more activity with DTT, which was similar to the mitochondrial MSRB2 from mammals. Ferredoxin (FD) is not the reducing system for Msrs, but might reflect the redox status in the cell and control the activity of Msrs indirectly.
Title: Characterization of Methionine Sulfoxide Reductases A and Bs from Tobacco Plant.
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Name(s): Ding, Di
Zhang, Xing-Hai, Thesis advisor
Florida Atlantic University, Degree grantor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Date Created: 2006
Date Issued: 2006
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 55 p.
Language(s): English
Summary: One methionine sulfoxide reductase A (TMSRA) and two methionine sulfoxide reductase Bs (TMSRB 1 and TMSRB2) were isolated from tobacco plants. TMSRA showed specificity for the reduction of Met-(S)-SO and both TMSRBs were specific for the reduction of Met-(R)-SO. TMSRA was the cytosolic form and both TMSRBs were plastid forms based on sequence comparison and expression tests. TMSRA and TMSRB2 could use either thioredoxin (TRX) or dithiothreitol (DTT) as reducing system, while TMSRB 1 showed little activity with TRX but much more activity with DTT, which was similar to the mitochondrial MSRB2 from mammals. Ferredoxin (FD) is not the reducing system for Msrs, but might reflect the redox status in the cell and control the activity of Msrs indirectly.
Identifier: FA00000746 (IID)
Collection: FAU Electronic Theses and Dissertations Collection
Note(s): Includes bibliography.
Thesis (M.S.)--Florida Atlantic University, 2006.
Charles E. Schmidt College of Science
Subject(s): Proteins--Chemical modification
Genetic regulation
Plant genetic engineering
Antioxidants
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00000746
Sublocation: Digital Library
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Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.