You are here

Rapid Analysis of Fecal Glucocorticoid Metabolites: Testing an Alternative Method for Analyzing Stress Markers in Chimpanzees

Download pdf | Full Screen View

Date Issued:
2016
Summary:
This study explores the application of two methods of spectroscopy; Near Infrared spectroscopy (NIR) and Fourier transform spectroscopy (FTIR) as alternative approaches for measuring glucocorticoid metabolites in chimpanzee feces. The goals of this study were twofold: The first was to determine if cortisol can be identified within the NIR and/or FTIR spectra of chimpanzee fecal hormone extract in ethanol solution. The second objective was to determine the capability of NIR and FTIR to predict FGM concentrations obtained using standard laboratory methods. Fecal glucocorticoid concentrations measured by Enzyme Immunoassay were used as the reference data of partial least square (PLS) regression of fecal extract NIR spectra and FTIR spectra. Low accuracies (NIR: R2 = 0.152; FTIR: R2 = 0.199) were obtained from regression models using data from both methods. Though this study did not successfully demonstrate the feasibility of using NIR and FTIR to qualify and quantify FGMs, it is likely not a reflection of the capabilities of the technology, but rather of appropriate sample types and preparation methods.
Title: Rapid Analysis of Fecal Glucocorticoid Metabolites: Testing an Alternative Method for Analyzing Stress Markers in Chimpanzees.
67 views
14 downloads
Name(s): Litman, Benni, author
Broadfield, Douglas C., Thesis advisor
Harris, Michael S., Thesis advisor
Florida Atlantic University, Degree grantor
Dorothy F. Schmidt College of Arts and Letters
Department of Anthropology
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Date Created: 2016
Date Issued: 2016
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 81 p.
Language(s): English
Summary: This study explores the application of two methods of spectroscopy; Near Infrared spectroscopy (NIR) and Fourier transform spectroscopy (FTIR) as alternative approaches for measuring glucocorticoid metabolites in chimpanzee feces. The goals of this study were twofold: The first was to determine if cortisol can be identified within the NIR and/or FTIR spectra of chimpanzee fecal hormone extract in ethanol solution. The second objective was to determine the capability of NIR and FTIR to predict FGM concentrations obtained using standard laboratory methods. Fecal glucocorticoid concentrations measured by Enzyme Immunoassay were used as the reference data of partial least square (PLS) regression of fecal extract NIR spectra and FTIR spectra. Low accuracies (NIR: R2 = 0.152; FTIR: R2 = 0.199) were obtained from regression models using data from both methods. Though this study did not successfully demonstrate the feasibility of using NIR and FTIR to qualify and quantify FGMs, it is likely not a reflection of the capabilities of the technology, but rather of appropriate sample types and preparation methods.
Identifier: FA00004723 (IID)
Degree granted: Thesis (M.A.)--Florida Atlantic University, 2016.
Collection: FAU Electronic Theses and Dissertations Collection
Note(s): Includes bibliography.
Subject(s): Biosensors
Infrared spectroscopy
Metabolites -- Spectra
Primates as laboratory animals
Spectrum analysis -- Diagnostic use
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Links: http://purl.flvc.org/fau/fd/FA00004723
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00004723
Use and Reproduction: Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.