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Rapid Analysis of Fecal Glucocorticoid Metabolites: Testing an Alternative Method for Analyzing Stress Markers in Chimpanzees
| Title: | Rapid Analysis of Fecal Glucocorticoid Metabolites: Testing an Alternative Method for Analyzing Stress Markers in Chimpanzees. |
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| Name(s): |
Litman, Benni, author Broadfield, Douglas C., Thesis advisor Harris, Michael S., Thesis advisor Florida Atlantic University, Degree grantor Dorothy F. Schmidt College of Arts and Letters Department of Anthropology |
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| Type of Resource: | text | |
| Genre: | Electronic Thesis Or Dissertation | |
| Date Created: | 2016 | |
| Date Issued: | 2016 | |
| Publisher: | Florida Atlantic University | |
| Place of Publication: | Boca Raton, Fla. | |
| Physical Form: | application/pdf | |
| Extent: | 81 p. | |
| Language(s): | English | |
| Summary: | This study explores the application of two methods of spectroscopy; Near Infrared spectroscopy (NIR) and Fourier transform spectroscopy (FTIR) as alternative approaches for measuring glucocorticoid metabolites in chimpanzee feces. The goals of this study were twofold: The first was to determine if cortisol can be identified within the NIR and/or FTIR spectra of chimpanzee fecal hormone extract in ethanol solution. The second objective was to determine the capability of NIR and FTIR to predict FGM concentrations obtained using standard laboratory methods. Fecal glucocorticoid concentrations measured by Enzyme Immunoassay were used as the reference data of partial least square (PLS) regression of fecal extract NIR spectra and FTIR spectra. Low accuracies (NIR: R2 = 0.152; FTIR: R2 = 0.199) were obtained from regression models using data from both methods. Though this study did not successfully demonstrate the feasibility of using NIR and FTIR to qualify and quantify FGMs, it is likely not a reflection of the capabilities of the technology, but rather of appropriate sample types and preparation methods. | |
| Identifier: | FA00004723 (IID) | |
| Degree granted: | Thesis (M.A.)--Florida Atlantic University, 2016. | |
| Collection: | FAU Electronic Theses and Dissertations Collection | |
| Note(s): | Includes bibliography. | |
| Subject(s): |
Biosensors Infrared spectroscopy Metabolites -- Spectra Primates as laboratory animals Spectrum analysis -- Diagnostic use |
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| Held by: | Florida Atlantic University Libraries | |
| Sublocation: | Digital Library | |
| Links: | http://purl.flvc.org/fau/fd/FA00004723 | |
| Persistent Link to This Record: | http://purl.flvc.org/fau/fd/FA00004723 | |
| Use and Reproduction: | Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
| Use and Reproduction: | http://rightsstatements.org/vocab/InC/1.0/ | |
| Host Institution: | FAU | |
| Is Part of Series: | Florida Atlantic University Digital Library Collections. |
