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Sponge cell culture for production of bioactive metabolites.

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Date Issued:
1994
Title: Sponge cell culture for production of bioactive metabolites.
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Name(s): Pomponi, Shirley A.
Willoughby, Robin
Type of Resource: text
Genre: Article
Date Issued: 1994
Publisher: A. A. Balkema.
Place of Publication: Brookfield, VT
Physical Form: pdf
Extent: 8 p.
Language(s): English
Identifier: FA00007381 (IID)
Note(s): Primary cell cultures of several species of shallow water marine demosponges have been initiatedto determine the feasibility of cell culture for bulk production of bioactive secondary metabolites. Selective cellenrichment and determination of cell-type specific localization of bioactive metabolites are accomplished bydensity gradient separation and chromatographic analyses of cell fractions. Microbial contamination has beencontrolled through the useofantibiotics . Analysis of dose-responsecurves for inhibition of microbial contaminationand effect on sponge cell viability has resulted in establishment of optimal antibiotic concentrations for cultureinitiation and maintenance. Physiological responses of cells are monitored by direct microscopic analysis, chromatographictechniques, colorimetric bioassays, and flow cytometric analyses of cell constituents and functions .Results of culture optimization studies to enhance biomass andlormetabolite production in Hymeniacidon heliophilaindicate that doubling rates can be significantly increased in response to mitogens, and cells will continue toproduce secondary metabolites in culture. These results suggest that sponge cell culture may be feasible for bulkproduction of bioactive secondary metabolites.
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution 985
This manuscript is an author version with the final publication available and may be cited as: Pomponi, S. A., & Willoughby, R. (1994). Sponge cell culture for production of bioactive metabolites. In R. W. M. van Soest, T. M. G. van Kempen, & J. C. Braekman (Eds.), Sponges in Time and Space: Biology, Chemistry, Paleontology: proceedings of the 4th International Porifera Congress, Amsterdam, Netherlands, 19-23 April 1993 (pp. 395-400). Rotterdam; Brookfield, VT: A. A. Balkema.
Subject(s): Sponges
Cell culture
Metabolites
Bioactive compounds
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00007381
Host Institution: FAU