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Development of techniques for in vitro production of bioactive natural products from marine sponges
| Title: | Development of techniques for in vitro production of bioactive natural products from marine sponges. |
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| Name(s): |
Pomponi, Shirley A. Willoughby, Robin Kaighn, M. E. Wright, Amy E. |
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| Type of Resource: | text | |
| Genre: | Article | |
| Date Issued: | 1997 | |
| Publisher: | Science Publishers | |
| Place of Publication: | Enfield, NH | |
| Physical Form: | ||
| Extent: | 8 p. | |
| Language(s): | English | |
| Identifier: | FA00007344 (IID) | |
| Note(s): |
Marine sponges are the source of many bioactive compounds with therapeutic potential. A critical issue
in the drug development strategy for marine natural products is ensuring an adequate supply of
compounds for clinical use while protecting the source organism and its habitat from over-exploitation.
One approach is the development of techniques for in vitro production of bioactive compounds.
Replicative cultures of the sponge Teichaxinella morchella have been established. Techniques for
monitoring sponge cell responses to growth factors by semi-automated microliter plate assays were
developed, including the sulforhodamine B method for total protein and a Hoechst 33342 DNA dye-binding
assay. The identity of cell cultures stimulated to divide by vertebrate growth factors and lectins was
verified by the in vitro production of stevensine (odiline), a compound which is ctiaracteristic of T.
morchella. Cultures retained their ability to synthesize stevensine after doubling, which demonstrates the
feasibility of in vitro production of bioactive, sponge-derived natural products. Florida Atlantic University. Harbor Branch Oceanographic Institute contribution 1172 This manuscript is an author version with the final publication available and may be cited as: Pomponi, S. A., Willoughby, R., Kaighn, M. E., & Wright, A. E. (1997). Development of techniques for in vitro production of bioactive natural products from marine sponges. In K. Maramorosch & J. Mitsuhashi (Eds.), Invertebrate cell culture: novel directions and biotechnology applications (pp. 231-237). Enfield, NH: Science Publishers. |
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| Subject(s): |
Sponges Bioactive compounds Marine natural products Cell culture Growth factors Phytohemagglutinins Cryopreservation |
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| Persistent Link to This Record: | http://purl.flvc.org/fau/fd/FA00007344 | |
| Host Institution: | FAU |
