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Phylogenetic resolution potential of 18S and 28S rRNA genes within the Iithistid Astrophorida.
| Title: | Phylogenetic resolution potential of 18S and 28S rRNA genes within the Iithistid Astrophorida. |
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| Name(s): |
McInerney, James O. Adams, Christi L. Kelly-Borges, M. Harbor Branch Oceanographic Institute |
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| Type of Resource: | text | |
| Genre: | Article | |
| Date Issued: | 1999 | |
| Publisher: | Queensland Museum | |
| Place of Publication: | Brisbane | |
| Physical Form: | ||
| Extent: | 10 p. | |
| Language(s): | English | |
| Identifier: | FA00007274 (IID) | |
| Note(s): |
Kelly-Borges et al. (1991) and Kelly-Borges & Pomponi (1994) utilised partial 18S rRNA
gene sequences to resolve relationships within hadromerid and lithistid sponges (Porifera:
Demospongiae). While their results clarified several specific systematic problems, their
conclusions were hampered by low levels of sequence variation. This study sought primarily
to evaluate the resolution potential between regions of the 18S rRNA gene used in previous
studies on sponges, and 28S rRNA genes used in more recent work. Six lithistid sponge taxa
were chosen to represent a gradient of taxonomic relationships, ranging through genus,
family, order and class. Approximately I ,300bp of the 18S rRNA gene and a 700bp region at
the 5' end of the 28S rRNA gene were compared with the data ofKelly-Borges & Pomponi
(1994). We found that the 700bp region of the 28S rRNA gene presented the greatest
potential for resolution of this group of Porifera at the genus and family level, and that the
resultant molecular phylogeny was congruent with morphological hypotheses for the group. Florida Atlantic University. Harbor Branch Oceanographic Institute contribution 1292 This manuscript is an author version with the final publication available and may be cited as: McInerney, J. O., Adams, C. L., & Kelly, M. (1999). Phylogenetic reso lution potential of 18S and 28S rRNA genes within the Iithistid Astrophorida. Memoirs of the Queensland Museum 44, 343-351. |
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| Subject(s): |
Sponges Phylogeny--Molecular aspects RNA, Ribosomal, 28S RNA, Ribosomal, 18S Demospongiae |
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| Persistent Link to This Record: | http://purl.flvc.org/fau/fd/FA00007274 | |
| Host Institution: | FAU |
