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Phylogenetic resolution potential of 18S and 28S rRNA genes within the Iithistid Astrophorida.

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Date Issued:
1999
Title: Phylogenetic resolution potential of 18S and 28S rRNA genes within the Iithistid Astrophorida.
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Name(s): McInerney, James O.
Adams, Christi L.
Kelly-Borges, M.
Harbor Branch Oceanographic Institute
Type of Resource: text
Genre: Article
Date Issued: 1999
Publisher: Queensland Museum
Place of Publication: Brisbane
Physical Form: pdf
Extent: 10 p.
Language(s): English
Identifier: FA00007274 (IID)
Note(s): Kelly-Borges et al. (1991) and Kelly-Borges & Pomponi (1994) utilised partial 18S rRNA gene sequences to resolve relationships within hadromerid and lithistid sponges (Porifera: Demospongiae). While their results clarified several specific systematic problems, their conclusions were hampered by low levels of sequence variation. This study sought primarily to evaluate the resolution potential between regions of the 18S rRNA gene used in previous studies on sponges, and 28S rRNA genes used in more recent work. Six lithistid sponge taxa were chosen to represent a gradient of taxonomic relationships, ranging through genus, family, order and class. Approximately I ,300bp of the 18S rRNA gene and a 700bp region at the 5' end of the 28S rRNA gene were compared with the data ofKelly-Borges & Pomponi (1994). We found that the 700bp region of the 28S rRNA gene presented the greatest potential for resolution of this group of Porifera at the genus and family level, and that the resultant molecular phylogeny was congruent with morphological hypotheses for the group.
Florida Atlantic University. Harbor Branch Oceanographic Institute contribution 1292
This manuscript is an author version with the final publication available and may be cited as: McInerney, J. O., Adams, C. L., & Kelly, M. (1999). Phylogenetic reso lution potential of 18S and 28S rRNA genes within the Iithistid Astrophorida. Memoirs of the Queensland Museum 44, 343-351.
Subject(s): Sponges
Phylogeny--Molecular aspects
RNA, Ribosomal, 28S
RNA, Ribosomal, 18S
Demospongiae
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00007274
Host Institution: FAU