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Improving In Vivo Two Photon Microscopy Without Adaptive Optics

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Date Issued:
2015
Summary:
Two photon microscopy is one of the fastest growing methods of in-vivo imaging of the brain. It has the capability of imaging structures on the scale of 1μm. At this scale the wavelength of the imaging field (usually near infra-red), is comparable to the size of the structures being imaged, which makes the use of ray optics invalid. A better understanding is needed to predict the result of introducing different media into the light path. We use Wolf's integral, which is capable of fulfilling these needs without the shortcomings of ray optics. We predict the effects of aberrating media introduced into the light path like glass cover-slips and then correct the aberration using the same method. We also create a method to predict aberrations when the interfaces of the media in the light-path are not aligned with the propagation direction of the wavefront.
Title: Improving In Vivo Two Photon Microscopy Without Adaptive Optics.
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Name(s): Estrada, Gerardo, author
Beetle, Christopher, Thesis advisor
Florida Atlantic University, Degree grantor
Charles E. Schmidt College of Science
Department of Physics
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Date Created: 2015
Date Issued: 2015
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 69 p.
Language(s): English
Summary: Two photon microscopy is one of the fastest growing methods of in-vivo imaging of the brain. It has the capability of imaging structures on the scale of 1μm. At this scale the wavelength of the imaging field (usually near infra-red), is comparable to the size of the structures being imaged, which makes the use of ray optics invalid. A better understanding is needed to predict the result of introducing different media into the light path. We use Wolf's integral, which is capable of fulfilling these needs without the shortcomings of ray optics. We predict the effects of aberrating media introduced into the light path like glass cover-slips and then correct the aberration using the same method. We also create a method to predict aberrations when the interfaces of the media in the light-path are not aligned with the propagation direction of the wavefront.
Identifier: FA00004495 (IID)
Degree granted: Dissertation (Ph.D.)--Florida Atlantic University, 2015.
Collection: FAU Electronic Theses and Dissertations Collection
Note(s): Includes bibliography.
Subject(s): Cellular signal transmission -- Measurement
Image analysis
Imaging systems in medicine
Membranes (Biology) -- Imaging
Neurons -- Imaging
Optics, Adaptive
Held by: Florida Atlantic University Libraries
Sublocation: Digital Library
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00004495
Use and Reproduction: Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.