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Myofibril-Inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl hearts
| Title: | Myofibril-Inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl hearts. |
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| Name(s): |
Zhang, Chi, creator Jia, Pingping, creator Huang, Xupei, creator Sferrazza, Gian Franco, creator Athauda, Gagani, creator Achary, Mohan P., creator Wang, Jikui, creator Lemanski, Sharon L., creator Lemanski, Larry F., creator |
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| Type of Resource: | text | |
| Date Issued: | 2009-09-03 | |
| Physical Description: | text/pdf[21p.] | |
| Language(s): | English | |
| Identifier: | 3327272 (digitool), FADT3327272 (IID), fau:1899 (fedora), 10.1186/1423-0127-16-81 (doi) | |
| FAU Department: | Department of Biomedical Sciences, Florida Atlantic University | |
| Note(s): | The Mexican axolotl, Ambystoma mexicanum, carries the naturally-occurring recessive mutant gene'c' that results in a failure of homozygous (c/c) embryos to form hearts that beat because of an absence of organized myofibrils. Our previous studies have shown that a noncoding RNA, Myofibril-Inducing RNA (MIR), is capable of promoting myofibrillogenesis and heart beating in the mutant (c/c) axolotls. The present study demonstrates that the MIR gene is essential for tropomyosin (TM) expression in axolotl hearts during development. Gene expression studies show that mRNA expression of various tropomyosin isoforms in untreated mutant hearts and in normal hearts knocked down with double-stranded MIR (dsMIR) are similar to untreated normal. However, at the protein level, selected tropomyosin isoforms are significantly reduced in mutantand dsMIR treated normal hearts. These results suggest that MIR is involved in controlling the translation or post-translation of various TM isoforms and subsequently of regulating cardiac contractility. | |
| Subject(s): |
Ambystoma mexicanum --embryology Ambystoma mexicanum --genetics Base Sequence DNA, Complementary --genetics Endoderm --physiology Exons --genetics Gene Expression Regulation, Developmental Gene Knockdown Techniques Genes, Recessive Heart --embryology Models, Genetic Molecular Sequence Data Muscle Proteins --biosynthesis Muscle Proteins --genetics Myocardial Contraction --physiology Protein Isoforms Reverse Transcriptase Polymerase Chain Reaction Tropomyosin |
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| Persistent Link to This Record: | http://purl.flvc.org/fcla/dt/3327272 | |
| Host Institution: | FAU |
