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Myofibril-Inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl hearts

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Title: Myofibril-Inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl hearts.
Name(s): Zhang, Chi, creator
Jia, Pingping, creator
Huang, Xupei, creator
Sferrazza, Gian Franco, creator
Athauda, Gagani, creator
Achary, Mohan P., creator
Wang, Jikui, creator
Lemanski, Sharon L., creator
Lemanski, Larry F., creator
Type of Resource: text
Date Issued: 2009-09-03
Physical Description: text/pdf[21p.]
Language(s): English
Identifier: 3327272 (digitool), FADT3327272 (IID), fau:1899 (fedora), 10.1186/1423-0127-16-81 (doi)
FAU Department: Department of Biomedical Sciences, Florida Atlantic University
Note(s): The Mexican axolotl, Ambystoma mexicanum, carries the naturally-occurring recessive mutant gene'c' that results in a failure of homozygous (c/c) embryos to form hearts that beat because of an absence of organized myofibrils. Our previous studies have shown that a noncoding RNA, Myofibril-Inducing RNA (MIR), is capable of promoting myofibrillogenesis and heart beating in the mutant (c/c) axolotls. The present study demonstrates that the MIR gene is essential for tropomyosin (TM) expression in axolotl hearts during development. Gene expression studies show that mRNA expression of various tropomyosin isoforms in untreated mutant hearts and in normal hearts knocked down with double-stranded MIR (dsMIR) are similar to untreated normal. However, at the protein level, selected tropomyosin isoforms are significantly reduced in mutantand dsMIR treated normal hearts. These results suggest that MIR is involved in controlling the translation or post-translation of various TM isoforms and subsequently of regulating cardiac contractility.
Subject(s): Ambystoma mexicanum --embryology
Ambystoma mexicanum --genetics
Base Sequence
DNA, Complementary --genetics
Endoderm --physiology
Exons --genetics
Gene Expression Regulation, Developmental
Gene Knockdown Techniques
Genes, Recessive
Heart --embryology
Models, Genetic
Molecular Sequence Data
Muscle Proteins --biosynthesis
Muscle Proteins --genetics
Myocardial Contraction --physiology
Protein Isoforms
Reverse Transcriptase Polymerase Chain Reaction
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Host Institution: FAU

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