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Assessment of multiple paternity for the queen conch, Strombus gigas

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Date Issued:
2008
Summary:
The commercially important queen conch, Strombus gigas, has been observed copulating with multiple partners and laying multiple egg masses during a reproductive season (Randall, 1964). While multiple paternity has been confirmed using microsatellite based genetic analysis for a variety of other gastropods, this technique has not been employed for S. gigas. Determining whether or not this species is capable of multiple paternity is important to understanding and maintaining genetic diversity of natural and captive populations. While an assessment of multiple paternity is the ultimate goal of this study, for my thesis, I have completed preliminary work which includes perfecting methods of tissue collection, DNA extraction, and DNA amplification with six non-labeled polymorphic microsatellite molecular markers, using cultured Strombus gigas animals. In addition, I collected tissue and extracted DNA from three wild S. gigas adult females and their egg masses from Pelican Shoal in the Florida Keys.
Title: Assessment of multiple paternity for the queen conch, Strombus gigas.
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Name(s): Harris, Chris.
Harriet L. Wilkes Honors College
Type of Resource: text
Genre: Thesis
Issuance: multipart monograph
Date Issued: 2008
Publisher: Florida Atlantic University
Physical Form: electronic
electronic resource
Extent: vii, 14 leaves : ill.
Language(s): English
Summary: The commercially important queen conch, Strombus gigas, has been observed copulating with multiple partners and laying multiple egg masses during a reproductive season (Randall, 1964). While multiple paternity has been confirmed using microsatellite based genetic analysis for a variety of other gastropods, this technique has not been employed for S. gigas. Determining whether or not this species is capable of multiple paternity is important to understanding and maintaining genetic diversity of natural and captive populations. While an assessment of multiple paternity is the ultimate goal of this study, for my thesis, I have completed preliminary work which includes perfecting methods of tissue collection, DNA extraction, and DNA amplification with six non-labeled polymorphic microsatellite molecular markers, using cultured Strombus gigas animals. In addition, I collected tissue and extracted DNA from three wild S. gigas adult females and their egg masses from Pelican Shoal in the Florida Keys.
Identifier: 297578853 (oclc), 77671 (digitool), FADT77671 (IID), fau:1491 (fedora)
Note(s): by Chris Harris.
Thesis (B.A.)--Florida Atlantic University, Honors College, 2008.
Bibliography: leaves 13-14.
Electronic reproduction. Boca Raton, FL : 2008 Mode of access: World Wide Web.
Subject(s): Gastropoda -- Habitat -- Florida Keys
Queen conch -- Conservation -- Florida Keys
Biological diversity
Marine biology
Mariculture -- Florida Keys
Held by: FBoU FAUER
Persistent Link to This Record: http://purl.flvc.org/FAU/77671
Use and Reproduction: Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Host Institution: FAU

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