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Optimizing atrazine catabolism in Pseudomonas sp. strain ADP

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Date Issued:
2010
Summary:
Atrazine is a popular herbicide used in over 80 countries to inhibit the growth of broadleaf and grassy weeds. Atrazine is a common pollutant in soil and groundwater, and high concentrations of atrazine cause developmental defects in fish, amphibians, and birds. The bacteria Pseudomonas sp. strain ADP (P.ADP) uses atrazine as a nitrogen source by transforming atrazine to ammonia through a number of enzymatic reactions. In this project I measured the growth and atrazine degradation of P.ADP in media with atrazine as the sole nitrogen source. A mutant strain isolated after mutagenesis with UV light showed faster growth and reached higher densities than the control strain. A series of mating experiments were performed to determine whether the growth mutation was on the atrazine degrading plasmid or in the chromosome. The limitations and potential of P.ADP for atrazine degradation are discussed.
Title: Optimizing atrazine catabolism in Pseudomonas sp. strain ADP.
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Name(s): Anderson, Robert.
Harriet L. Wilkes Honors College
Type of Resource: text
Genre: Thesis
Issuance: multipart monograph
Date Issued: 2010
Publisher: Florida Atlantic University
Physical Form: electronic resource
Extent: vi, 24 p. : ill. (some col.)
Language(s): English
Summary: Atrazine is a popular herbicide used in over 80 countries to inhibit the growth of broadleaf and grassy weeds. Atrazine is a common pollutant in soil and groundwater, and high concentrations of atrazine cause developmental defects in fish, amphibians, and birds. The bacteria Pseudomonas sp. strain ADP (P.ADP) uses atrazine as a nitrogen source by transforming atrazine to ammonia through a number of enzymatic reactions. In this project I measured the growth and atrazine degradation of P.ADP in media with atrazine as the sole nitrogen source. A mutant strain isolated after mutagenesis with UV light showed faster growth and reached higher densities than the control strain. A series of mating experiments were performed to determine whether the growth mutation was on the atrazine degrading plasmid or in the chromosome. The limitations and potential of P.ADP for atrazine degradation are discussed.
Identifier: 779176633 (oclc), 3334252 (digitool), FADT3334252 (IID), fau:1391 (fedora)
Note(s): by Robert Anderson.
Signature page is unsigned.
Thesis (B.A.)--Florida Atlantic University, Honors College, 2010.
Includes bibliography.
Electronic reproduction. Boca Raton, Fla., 2010. Mode of access: World Wide Web.
Subject(s): Plasmids -- Genetics
Alginates -- Physiological effect
Microbial ecology
Biotransformation (Metabolism)
Held by: FBoU FAUER
Persistent Link to This Record: http://purl.flvc.org/FAU/3334252
Use and Reproduction: Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Host Institution: FAU

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