You are here

tRNA processing and quality control in bacteria

Download pdf | Full Screen View

Date Issued:
2013
Summary:
In this work, we report that the only exoribonuclease in M. genitalium, RNase R, is able to generate mature 3'-ends. The aminoacyl-acceptor stem, CCA terminus and discriminator residue plays an important role in stopping RNase R digestion at the mature 3'-end. Disruption of the stem causes partial or complete degradation of the pre-tRNA, whereas extension of the stem results in the formation of a mature 3’-end. CC residues in CCA terminus and A or G residues at discriminator position are the most preferred residues for precise stopping of RNase R at mature 3’ end. The significance of this works shows that M. genitalium RNase R generates mature tRNA in a single step by recognizing features in the terminal domains of tRNA, a process requiring multiple RNases in most bacteria.
Title: tRNA processing and quality control in bacteria.
149 views
80 downloads
Name(s): Alluri, Ravi Kumar, author
Li, Zhongwei, Thesis advisor
Charles E. Schmidt College of Science, Degree grantor
Department of Biomedical Science
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Date Created: Spring 2013
Date Issued: 2013
Publisher: Florida Atlantic University
Physical Form: Online Resource
Extent: 158 p.
Language(s): English
Summary: In this work, we report that the only exoribonuclease in M. genitalium, RNase R, is able to generate mature 3'-ends. The aminoacyl-acceptor stem, CCA terminus and discriminator residue plays an important role in stopping RNase R digestion at the mature 3'-end. Disruption of the stem causes partial or complete degradation of the pre-tRNA, whereas extension of the stem results in the formation of a mature 3’-end. CC residues in CCA terminus and A or G residues at discriminator position are the most preferred residues for precise stopping of RNase R at mature 3’ end. The significance of this works shows that M. genitalium RNase R generates mature tRNA in a single step by recognizing features in the terminal domains of tRNA, a process requiring multiple RNases in most bacteria.
Identifier: FA00004174 (IID)
Note(s): Includes bibliography.
Dissertation (Ph.D.)--Florida Atlantic University, 2013.
Held by: Florida Atlantic University Digital Library
Sublocation: Boca Raton, Fla.
Persistent Link to This Record: http://purl.flvc.org/fau/fd/FA00004174
Restrictions on Access: All rights reserved by the source institution
Restrictions on Access: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU