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tRNA processing and quality control in bacteria
- Date Issued:
- 2013
- Summary:
- In this work, we report that the only exoribonuclease in M. genitalium, RNase R, is able to generate mature 3'-ends. The aminoacyl-acceptor stem, CCA terminus and discriminator residue plays an important role in stopping RNase R digestion at the mature 3'-end. Disruption of the stem causes partial or complete degradation of the pre-tRNA, whereas extension of the stem results in the formation of a mature 3’-end. CC residues in CCA terminus and A or G residues at discriminator position are the most preferred residues for precise stopping of RNase R at mature 3’ end. The significance of this works shows that M. genitalium RNase R generates mature tRNA in a single step by recognizing features in the terminal domains of tRNA, a process requiring multiple RNases in most bacteria.
Title: | tRNA processing and quality control in bacteria. |
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Name(s): |
Alluri, Ravi Kumar, author Li, Zhongwei, Thesis advisor Charles E. Schmidt College of Science, Degree grantor Department of Biomedical Science |
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Type of Resource: | text | |
Genre: | Electronic Thesis Or Dissertation | |
Date Created: | Spring 2013 | |
Date Issued: | 2013 | |
Publisher: | Florida Atlantic University | |
Physical Form: | Online Resource | |
Extent: | 158 p. | |
Language(s): | English | |
Summary: | In this work, we report that the only exoribonuclease in M. genitalium, RNase R, is able to generate mature 3'-ends. The aminoacyl-acceptor stem, CCA terminus and discriminator residue plays an important role in stopping RNase R digestion at the mature 3'-end. Disruption of the stem causes partial or complete degradation of the pre-tRNA, whereas extension of the stem results in the formation of a mature 3’-end. CC residues in CCA terminus and A or G residues at discriminator position are the most preferred residues for precise stopping of RNase R at mature 3’ end. The significance of this works shows that M. genitalium RNase R generates mature tRNA in a single step by recognizing features in the terminal domains of tRNA, a process requiring multiple RNases in most bacteria. | |
Identifier: | FA00004174 (IID) | |
Note(s): |
Includes bibliography. Dissertation (Ph.D.)--Florida Atlantic University, 2013. |
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Held by: | Florida Atlantic University Digital Library | |
Sublocation: | Boca Raton, Fla. | |
Persistent Link to This Record: | http://purl.flvc.org/fau/fd/FA00004174 | |
Restrictions on Access: | All rights reserved by the source institution | |
Restrictions on Access: | http://rightsstatements.org/vocab/InC/1.0/ | |
Host Institution: | FAU |