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Construction of mitochondrion-targeted telomerase for analysis in Saccharomyces cerevisiae
- Date Issued:
- 2009
- Summary:
- Telomerase is associated with telomere production and nDNA protection. However, studies by Santos et al. have demonstrated that human telomerase has a mitochondrial entry sequence and in the presence of hydrogen peroxide it has been found inside the mitochondrion and may cause mitochondrial DNA mutations. Saccharomyces cerevisiae contains telomerase, but it does not have the mitochondrial entry sequence. To determine if the presence of telomerase in the mitochondria can induce mutations an experiment was developed in which a mitochondrion entry sequence would be fused to the S. cerevisiae telomerase enzyme. This fusion could then be screened in S. cerevisiae with an ade2 mutation for a simple color assay of mitochondrial activity. To date, no successful transformant has been identified. The frequency of incorrect ligations has been recognized and may indicate that the desired fusion is lethal to E. coli cells.
Title: | Construction of mitochondrion-targeted telomerase for analysis in Saccharomyces cerevisiae. |
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Name(s): |
Martin, Ricardo. Harriet L. Wilkes Honors College |
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Type of Resource: | text | |
Genre: | Thesis | |
Issuance: | multipart monograph | |
Date Issued: | 2009 | |
Publisher: | Florida Atlantic University | |
Physical Form: |
electronic electronic resource |
|
Extent: | vii, 32 leaves : ill. (some col.) ; 29 cm. | |
Language(s): | English | |
Summary: | Telomerase is associated with telomere production and nDNA protection. However, studies by Santos et al. have demonstrated that human telomerase has a mitochondrial entry sequence and in the presence of hydrogen peroxide it has been found inside the mitochondrion and may cause mitochondrial DNA mutations. Saccharomyces cerevisiae contains telomerase, but it does not have the mitochondrial entry sequence. To determine if the presence of telomerase in the mitochondria can induce mutations an experiment was developed in which a mitochondrion entry sequence would be fused to the S. cerevisiae telomerase enzyme. This fusion could then be screened in S. cerevisiae with an ade2 mutation for a simple color assay of mitochondrial activity. To date, no successful transformant has been identified. The frequency of incorrect ligations has been recognized and may indicate that the desired fusion is lethal to E. coli cells. | |
Identifier: | 461340391 (oclc), 209994 (digitool), FADT209994 (IID), fau:1368 (fedora) | |
Note(s): |
by Ricardo Martin. Thesis (B.A.)--Florida Atlantic University, Honors College, 2009. Bibliography: leaves 30-32. Electronic reproduction. Boca Raton, Fla., 2009. Mode of access: World Wide Web. |
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Subject(s): |
Cell membranes -- Formation Mitochondrial DNA Mutation (Biology) Cell metabolism |
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Held by: | FBoU FAUER | |
Persistent Link to This Record: | http://purl.flvc.org/FAU/209994 | |
Use and Reproduction: | Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
Host Institution: | FAU |