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study of conjugated monoclonal antibodies in an immunoassay for fish species identification

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Date Issued:
2000
Summary:
Atlantic billfishes (family Istiophoridae) are overexploited and often illegally harvested. To address both of these problems, a rapid means of identifying billfish carcasses is needed. This thesis describes a simple and rapid Nalge Nunc-Immuno(TM) Stick-based direct sandwich assay for sailfish identification that can be performed in the field. A species-specific anti-sailfish monoclonal antibody, covalently bound to the Nalge Nunc-Immuno(TM) Stick's polystyrene surface, was used to capture a biomarker molecule---serum albumin---from sailfish tissue samples. This antibody-antigen interaction was visualized by utilizing peroxidase-conjugated anti-billfish monoclonal antibodies or polyclonal antibodies together with a precipitating substrate. This technique successfully differentiated between sailfish and other fishes within 15 minutes, with a high degree of sensitivity and accuracy. This assay has many potential applications for species identification.
Title: A study of conjugated monoclonal antibodies in an immunoassay for fish species identification.
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Name(s): Yu, Wenjie
Florida Atlantic University, Degree Grantor
Hartmann, James X., Thesis Advisor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 2000
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 58 p.
Language(s): English
Summary: Atlantic billfishes (family Istiophoridae) are overexploited and often illegally harvested. To address both of these problems, a rapid means of identifying billfish carcasses is needed. This thesis describes a simple and rapid Nalge Nunc-Immuno(TM) Stick-based direct sandwich assay for sailfish identification that can be performed in the field. A species-specific anti-sailfish monoclonal antibody, covalently bound to the Nalge Nunc-Immuno(TM) Stick's polystyrene surface, was used to capture a biomarker molecule---serum albumin---from sailfish tissue samples. This antibody-antigen interaction was visualized by utilizing peroxidase-conjugated anti-billfish monoclonal antibodies or polyclonal antibodies together with a precipitating substrate. This technique successfully differentiated between sailfish and other fishes within 15 minutes, with a high degree of sensitivity and accuracy. This assay has many potential applications for species identification.
Identifier: 9780599747388 (isbn), 15794 (digitool), FADT15794 (IID), fau:12546 (fedora)
Note(s): Thesis (M.S.)--Florida Atlantic University, 2000.
Subject(s): Monoclonal antibodies
Fishes--Identification
Fishes--Immunology
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/15794
Sublocation: Digital Library
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.