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Isolation and characterization of chlorophyll and carotenoids in Florida Bay: Phytoplankton, microphytobenthos and sediments

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Date Issued:
1999
Summary:
An analysis of the waters, microphytobenthos, and carbonate marl sediments of Florida Bay was performed using ion-paired RP-HPLC-PDA for chemotaxonomic characterization of past and present photoautotrophic, communities. Application of regression formulae was determined to be applicable for the waters, microphytobenthos, and surficial sediments (0--5 cm) of Florida Bay using chemotaxonomic principles. Loss of chemotaxonomically important pigments with depth prevented similar application in deeper strata (>5 cm), however, past oxic and anoxic trends could be determined. Sedimentary alteration of pigments are characterized by: rapid conversion of chlorophylls-alpha and bacteriochlorophylls-alpha to their respective pheophytins-alpha, creation of a 'carotenoid diol-cluster', equilibrium isomerization of beta-carotene (15-cis and trans), and rapid conversion of pyropheophorbide-alpha to cyclopheophorbide-alpha. An abundant presence of pyropheophorbide-alpha steryl/hopanoid esters and an unusual relationship between organic carbon and water content providing the sediments with polymeric characteristics were also found.
Title: Isolation and characterization of chlorophyll and carotenoids in Florida Bay: Phytoplankton, microphytobenthos and sediments.
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Name(s): Loitz, Joseph William
Florida Atlantic University, Degree Grantor
Louda, J. William, Thesis Advisor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 1999
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 232 p.
Language(s): English
Summary: An analysis of the waters, microphytobenthos, and carbonate marl sediments of Florida Bay was performed using ion-paired RP-HPLC-PDA for chemotaxonomic characterization of past and present photoautotrophic, communities. Application of regression formulae was determined to be applicable for the waters, microphytobenthos, and surficial sediments (0--5 cm) of Florida Bay using chemotaxonomic principles. Loss of chemotaxonomically important pigments with depth prevented similar application in deeper strata (>5 cm), however, past oxic and anoxic trends could be determined. Sedimentary alteration of pigments are characterized by: rapid conversion of chlorophylls-alpha and bacteriochlorophylls-alpha to their respective pheophytins-alpha, creation of a 'carotenoid diol-cluster', equilibrium isomerization of beta-carotene (15-cis and trans), and rapid conversion of pyropheophorbide-alpha to cyclopheophorbide-alpha. An abundant presence of pyropheophorbide-alpha steryl/hopanoid esters and an unusual relationship between organic carbon and water content providing the sediments with polymeric characteristics were also found.
Identifier: 9780599540637 (isbn), 15734 (digitool), FADT15734 (IID), fau:12490 (fedora)
Note(s): Thesis (M.S.)--Florida Atlantic University, 1999.
Subject(s): Marine sediments--Florida--Florida Bay
Carotenoids--Analysis
Chlorophyll--Analysis
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/15734
Sublocation: Digital Library
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.