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Biosynthesis and enzymology of secosteroids from Pseudopterogorgia americana
- Date Issued:
- 1999
- Summary:
- 9,11-Secosteroids are biologically active secondary metabolites from the marine invertebrate, Pseudopterogorgia americana. They are produced in vitro through an incubation of the steroid substrate with a cell-free extract of P. americana containing the necessary enzymes. Further optimization of this incubation was attempted through adjustment of incubation reagents and cofactors. The enzymes responsible for secosteroid production were partially purified through chromatography in an effort to isolate and purify these enzymes. Finally, experiments with radiolabeled gorgosterol led to the elucidation and isolation of a key secosteroid precursor, 9,11-dihydroxygorgosterol.
Title: | Biosynthesis and enzymology of secosteroids from Pseudopterogorgia americana. |
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Name(s): |
Head, Kevin Florida Atlantic University, Degree Grantor Kerr, Russell G., Thesis Advisor |
|
Type of Resource: | text | |
Genre: | Electronic Thesis Or Dissertation | |
Issuance: | monographic | |
Date Issued: | 1999 | |
Publisher: | Florida Atlantic University | |
Place of Publication: | Boca Raton, Fla. | |
Physical Form: | application/pdf | |
Extent: | 83 p. | |
Language(s): | English | |
Summary: | 9,11-Secosteroids are biologically active secondary metabolites from the marine invertebrate, Pseudopterogorgia americana. They are produced in vitro through an incubation of the steroid substrate with a cell-free extract of P. americana containing the necessary enzymes. Further optimization of this incubation was attempted through adjustment of incubation reagents and cofactors. The enzymes responsible for secosteroid production were partially purified through chromatography in an effort to isolate and purify these enzymes. Finally, experiments with radiolabeled gorgosterol led to the elucidation and isolation of a key secosteroid precursor, 9,11-dihydroxygorgosterol. | |
Identifier: | 9780599537057 (isbn), 15711 (digitool), FADT15711 (IID), fau:12467 (fedora) | |
Note(s): | Thesis (M.S.)--Florida Atlantic University, 1999. | |
Subject(s): |
Alcyonacea Enzymes--Synthesis Sterols |
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Held by: | Florida Atlantic University Libraries | |
Persistent Link to This Record: | http://purl.flvc.org/fcla/dt/15711 | |
Sublocation: | Digital Library | |
Use and Reproduction: | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
Use and Reproduction: | http://rightsstatements.org/vocab/InC/1.0/ | |
Host Institution: | FAU | |
Is Part of Series: | Florida Atlantic University Digital Library Collections. |