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Investigation of dynamically coated alumina stationary phases for reversed-phase high-performance liquid chromatography

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Date Issued:
1999
Summary:
Dynamically coated alumina (DCA) was prepared by pumping mobile phase containing methanol and concentration of 10mM Sodium Lauryl Sulphate (SLS), an anionic surfactant prepared in water, through a bare alumina column. The mechanism of separation of a test mixture were characteristic of reversed phase chromatography as evident in the decreased retention time as the percentage of methanol increased. However, the effects of changing the pH of the mobile phase on retention of solutes were different than that expected in reversed phase separations, indicating the presence of another retention mechanism. Excellent resolutions, peak shapes and peak symmetries were obtained using the DCA conditions and were comparable to similar separations performed on an ODA (octadecylalumina) column. Comparisons of retentions of a variety of organic compounds on DCA with those of commercial ODS (octadecylsilica) and ODA phases were also performed.
Title: Investigation of dynamically coated alumina stationary phases for reversed-phase high-performance liquid chromatography.
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Name(s): Ferguson, Veronica
Florida Atlantic University, Degree Grantor
Haky, Jerome E., Thesis Advisor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 1999
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 90 p.
Language(s): English
Summary: Dynamically coated alumina (DCA) was prepared by pumping mobile phase containing methanol and concentration of 10mM Sodium Lauryl Sulphate (SLS), an anionic surfactant prepared in water, through a bare alumina column. The mechanism of separation of a test mixture were characteristic of reversed phase chromatography as evident in the decreased retention time as the percentage of methanol increased. However, the effects of changing the pH of the mobile phase on retention of solutes were different than that expected in reversed phase separations, indicating the presence of another retention mechanism. Excellent resolutions, peak shapes and peak symmetries were obtained using the DCA conditions and were comparable to similar separations performed on an ODA (octadecylalumina) column. Comparisons of retentions of a variety of organic compounds on DCA with those of commercial ODS (octadecylsilica) and ODA phases were also performed.
Identifier: 9780599372849 (isbn), 15673 (digitool), FADT15673 (IID), fau:12429 (fedora)
Note(s): Thesis (M.S.)--Florida Atlantic University, 1999.
Subject(s): High performance liquid chromatography
Aluminum coating
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/15673
Sublocation: Digital Library
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Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.