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Monoclonal antibodies and blue marlin (Makaira nigricans) identification

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Date Issued:
1996
Summary:
Spleenocytes from Balb/c mouse immunized with purified blue marlin albumin were fused with X 63 myeloma cells in a polyethylene glycol aided fusion. The resulting fusomas were screened for anti-blue marlin activity and a potential blue marlin specific hybridoma clone designated 605D was isolated and expanded in cell culture. While testing the reactivity of monoclonal antibody secreted by clone 605D against pooled serum samples of blue marlin, white marlin and sailfish in indirect ELISA, 605D could differentiate blue marlin from white marlin and sailfish clearly. The crossreactivity to white marlin was minimised by diluting sera samples and antibody solutions. However, when individual billfish serum samples and heart tissue homogenates were tested 605D could not differentiate between the two marlin species, though crossreactivity to SF was minimal.
Title: Monoclonal antibodies and blue marlin (Makaira nigricans) identification.
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Name(s): Paniker, Lakshmi.
Florida Atlantic University, Degree grantor
Hartmann, James X., Thesis advisor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 1996
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 57 p.
Language(s): English
Summary: Spleenocytes from Balb/c mouse immunized with purified blue marlin albumin were fused with X 63 myeloma cells in a polyethylene glycol aided fusion. The resulting fusomas were screened for anti-blue marlin activity and a potential blue marlin specific hybridoma clone designated 605D was isolated and expanded in cell culture. While testing the reactivity of monoclonal antibody secreted by clone 605D against pooled serum samples of blue marlin, white marlin and sailfish in indirect ELISA, 605D could differentiate blue marlin from white marlin and sailfish clearly. The crossreactivity to white marlin was minimised by diluting sera samples and antibody solutions. However, when individual billfish serum samples and heart tissue homogenates were tested 605D could not differentiate between the two marlin species, though crossreactivity to SF was minimal.
Identifier: 9780591161496 (isbn), 15347 (digitool), FADT15347 (IID), fau:12115 (fedora)
Note(s): Charles E. Schmidt College of Science
Thesis (M.S.)--Florida Atlantic University, 1996.
Subject(s): Blue marlin--Identification
Fishes--Immunology
Immunology--Technique
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/15347
Sublocation: Digital Library
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.