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Monoclonal antibodies and blue marlin (Makaira nigricans) identification
| Title: | Monoclonal antibodies and blue marlin (Makaira nigricans) identification. |
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| Name(s): |
Paniker, Lakshmi. Florida Atlantic University, Degree grantor Hartmann, James X., Thesis advisor |
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| Type of Resource: | text | |
| Genre: | Electronic Thesis Or Dissertation | |
| Issuance: | monographic | |
| Date Issued: | 1996 | |
| Publisher: | Florida Atlantic University | |
| Place of Publication: | Boca Raton, Fla. | |
| Physical Form: | application/pdf | |
| Extent: | 57 p. | |
| Language(s): | English | |
| Summary: | Spleenocytes from Balb/c mouse immunized with purified blue marlin albumin were fused with X 63 myeloma cells in a polyethylene glycol aided fusion. The resulting fusomas were screened for anti-blue marlin activity and a potential blue marlin specific hybridoma clone designated 605D was isolated and expanded in cell culture. While testing the reactivity of monoclonal antibody secreted by clone 605D against pooled serum samples of blue marlin, white marlin and sailfish in indirect ELISA, 605D could differentiate blue marlin from white marlin and sailfish clearly. The crossreactivity to white marlin was minimised by diluting sera samples and antibody solutions. However, when individual billfish serum samples and heart tissue homogenates were tested 605D could not differentiate between the two marlin species, though crossreactivity to SF was minimal. | |
| Identifier: | 9780591161496 (isbn), 15347 (digitool), FADT15347 (IID), fau:12115 (fedora) | |
| Note(s): |
Charles E. Schmidt College of Science Thesis (M.S.)--Florida Atlantic University, 1996. |
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| Subject(s): |
Blue marlin--Identification Fishes--Immunology Immunology--Technique |
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| Held by: | Florida Atlantic University Libraries | |
| Persistent Link to This Record: | http://purl.flvc.org/fcla/dt/15347 | |
| Sublocation: | Digital Library | |
| Use and Reproduction: | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
| Use and Reproduction: | http://rightsstatements.org/vocab/InC/1.0/ | |
| Host Institution: | FAU | |
| Is Part of Series: | Florida Atlantic University Digital Library Collections. |
