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Novel DNA sequences of the endangered Florida Semaphore cactus
- Date Issued:
- 1996
- Summary:
- The population decline in the Florida Semaphore cactus, Opuntia corallicola, due to grazing by the moth, Cactoblastus cactorum, has placed this plant species on the verge of extinction in the Florida Keys. Although this semaphore cactus is endemic to Florida, it was mis-named as an almost uncommon Jamaican species (Opuntia spinosissima) in the 1970s. Our goal was to provide genetic data to distinguish these cacti with nuclear and chloropast genes previously used in other plant phylogenetic analyses. Phylogenetic relationships were inferred from nucleotide sequence variation in both the Leucine-Phenylalanine transfer RNA intergenic spacer (trnL-trnF) and ribulose bisphosphate carboxylase-oxygenase (rbcL) genes of cactus chloropast DNA. While the trnL-trnF gene sequence data could only group cacti at the genus level, it was the data from the rbcL gene which show speciation and differences between the Florida Semaphore cactus, Opuntia corallicola, and the Jamaican species, Opuntia spinosissima. Sequence data from the internal transcribed spacer (ITS) regions of nuclear DNA were not available due to the divergence of the cactus ITS region from the universal primers sequences used to initiate the polymerase chain reaction (PCR).
Title: | Novel DNA sequences of the endangered Florida Semaphore cactus. |
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Name(s): |
Dougherty, Christopher James. Florida Atlantic University, Degree grantor Binninger, David, Thesis advisor |
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Type of Resource: | text | |
Genre: | Electronic Thesis Or Dissertation | |
Issuance: | monographic | |
Date Issued: | 1996 | |
Publisher: | Florida Atlantic University | |
Place of Publication: | Boca Raton, Fla. | |
Physical Form: | application/pdf | |
Extent: | 108 p. | |
Language(s): | English | |
Summary: | The population decline in the Florida Semaphore cactus, Opuntia corallicola, due to grazing by the moth, Cactoblastus cactorum, has placed this plant species on the verge of extinction in the Florida Keys. Although this semaphore cactus is endemic to Florida, it was mis-named as an almost uncommon Jamaican species (Opuntia spinosissima) in the 1970s. Our goal was to provide genetic data to distinguish these cacti with nuclear and chloropast genes previously used in other plant phylogenetic analyses. Phylogenetic relationships were inferred from nucleotide sequence variation in both the Leucine-Phenylalanine transfer RNA intergenic spacer (trnL-trnF) and ribulose bisphosphate carboxylase-oxygenase (rbcL) genes of cactus chloropast DNA. While the trnL-trnF gene sequence data could only group cacti at the genus level, it was the data from the rbcL gene which show speciation and differences between the Florida Semaphore cactus, Opuntia corallicola, and the Jamaican species, Opuntia spinosissima. Sequence data from the internal transcribed spacer (ITS) regions of nuclear DNA were not available due to the divergence of the cactus ITS region from the universal primers sequences used to initiate the polymerase chain reaction (PCR). | |
Identifier: | 9780591159929 (isbn), 15335 (digitool), FADT15335 (IID), fau:12103 (fedora) | |
Collection: | FAU Electronic Theses and Dissertations Collection | |
Note(s): |
Thesis (M.S.)--Florida Atlantic University, 1996. Charles E. Schmidt College of Science |
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Subject(s): |
Cactus--Classification Plant cytotaxonomy Opuntia |
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Held by: | Florida Atlantic University Libraries | |
Persistent Link to This Record: | http://purl.flvc.org/fcla/dt/15335 | |
Sublocation: | Digital Library | |
Use and Reproduction: | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
Use and Reproduction: | http://rightsstatements.org/vocab/InC/1.0/ | |
Host Institution: | FAU | |
Is Part of Series: | Florida Atlantic University Digital Library Collections. |