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Comparison of alkyl-bonded alumina and silica stationary phases for peptide and protein separations by high performance liquid chromatography
- Date Issued:
- 1996
- Summary:
- The applicability of a recently developed octadecylalumina (ODA) stationary phase for the preparative separations of proteins and peptides is compared with that of conventional Octadecylsilica (ODS) phases. Chromatographic peak widths, peaks areas, and recoveries of polypeptides were obtained from both types of phases. The ODA phase compares favorably with the ODS phase on small peptides, but exhibits low recoveries on high molecular weight proteins. The results are attributed to the unique fused-microplatelet shape of the ODA particles.
Title: | Comparison of alkyl-bonded alumina and silica stationary phases for peptide and protein separations by high performance liquid chromatography. |
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Name(s): |
Williams, David Clinton Florida Atlantic University, Degree Grantor Haky, Jerome E., Thesis Advisor |
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Type of Resource: | text | |
Genre: | Electronic Thesis Or Dissertation | |
Issuance: | monographic | |
Date Issued: | 1996 | |
Publisher: | Florida Atlantic University | |
Place of Publication: | Boca Raton, Fla. | |
Physical Form: | application/pdf | |
Extent: | 77 p. | |
Language(s): | English | |
Summary: | The applicability of a recently developed octadecylalumina (ODA) stationary phase for the preparative separations of proteins and peptides is compared with that of conventional Octadecylsilica (ODS) phases. Chromatographic peak widths, peaks areas, and recoveries of polypeptides were obtained from both types of phases. The ODA phase compares favorably with the ODS phase on small peptides, but exhibits low recoveries on high molecular weight proteins. The results are attributed to the unique fused-microplatelet shape of the ODA particles. | |
Identifier: | 15260 (digitool), FADT15260 (IID), fau:12031 (fedora) | |
Note(s): | Thesis (M.S.)--Florida Atlantic University, 1996. | |
Subject(s): |
High performance liquid chromatography Proteins--Separation Peptides--Separation |
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Held by: | Florida Atlantic University Libraries | |
Persistent Link to This Record: | http://purl.flvc.org/fcla/dt/15260 | |
Sublocation: | Digital Library | |
Use and Reproduction: | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
Use and Reproduction: | http://rightsstatements.org/vocab/InC/1.0/ | |
Host Institution: | FAU | |
Is Part of Series: | Florida Atlantic University Digital Library Collections. |