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Comparison of alkyl-bonded alumina and silica stationary phases for peptide and protein separations by high performance liquid chromatography

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Date Issued:
1996
Summary:
The applicability of a recently developed octadecylalumina (ODA) stationary phase for the preparative separations of proteins and peptides is compared with that of conventional Octadecylsilica (ODS) phases. Chromatographic peak widths, peaks areas, and recoveries of polypeptides were obtained from both types of phases. The ODA phase compares favorably with the ODS phase on small peptides, but exhibits low recoveries on high molecular weight proteins. The results are attributed to the unique fused-microplatelet shape of the ODA particles.
Title: Comparison of alkyl-bonded alumina and silica stationary phases for peptide and protein separations by high performance liquid chromatography.
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Name(s): Williams, David Clinton
Florida Atlantic University, Degree Grantor
Haky, Jerome E., Thesis Advisor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 1996
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 77 p.
Language(s): English
Summary: The applicability of a recently developed octadecylalumina (ODA) stationary phase for the preparative separations of proteins and peptides is compared with that of conventional Octadecylsilica (ODS) phases. Chromatographic peak widths, peaks areas, and recoveries of polypeptides were obtained from both types of phases. The ODA phase compares favorably with the ODS phase on small peptides, but exhibits low recoveries on high molecular weight proteins. The results are attributed to the unique fused-microplatelet shape of the ODA particles.
Identifier: 15260 (digitool), FADT15260 (IID), fau:12031 (fedora)
Note(s): Thesis (M.S.)--Florida Atlantic University, 1996.
Subject(s): High performance liquid chromatography
Proteins--Separation
Peptides--Separation
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/15260
Sublocation: Digital Library
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.