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Rapid, immunologic identification of istiophorid fishes from minute tissue samples

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Date Issued:
1994
Summary:
A monoclonal antibody (MAb) produced against sailfish (Istiophorus albicans) serum albumin (SFA)/(Rossi et al. 1992) was used in conjunction with rabbit polyclonal antibodies (PAbs) to formulate a sandwich-style enzyme immunoassay (sEIA). The sEIA specifically identifies sailfish tissues utilizing samples such as heart, kidney, white muscle, red muscle, and blood serum. The assay is sensitive to 20ng of SFA. Of the tissues tested, kidney yields the strongest signal. The entire assay is carried out in a 3cc syringe and, excluding sample preparation, can be executed in 30 minutes. No specialized equipment or training is required. MAbs were also produced against serum albumin purified from white marlin (Tetrapturus albidus) and Atlantic blue marlin (Makaira nigricans). These Mabs were examined for their utility in identification assays for these species.
Title: Rapid, immunologic identification of istiophorid fishes from minute tissue samples.
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Name(s): Shepard, Scot Robert.
Florida Atlantic University, Degree grantor
Hartmann, James X., Thesis advisor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 1994
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 92 p.
Language(s): English
Summary: A monoclonal antibody (MAb) produced against sailfish (Istiophorus albicans) serum albumin (SFA)/(Rossi et al. 1992) was used in conjunction with rabbit polyclonal antibodies (PAbs) to formulate a sandwich-style enzyme immunoassay (sEIA). The sEIA specifically identifies sailfish tissues utilizing samples such as heart, kidney, white muscle, red muscle, and blood serum. The assay is sensitive to 20ng of SFA. Of the tissues tested, kidney yields the strongest signal. The entire assay is carried out in a 3cc syringe and, excluding sample preparation, can be executed in 30 minutes. No specialized equipment or training is required. MAbs were also produced against serum albumin purified from white marlin (Tetrapturus albidus) and Atlantic blue marlin (Makaira nigricans). These Mabs were examined for their utility in identification assays for these species.
Identifier: 15052 (digitool), FADT15052 (IID), fau:11830 (fedora)
Note(s): Charles E. Schmidt College of Science
Thesis (M.S.)--Florida Atlantic University, 1994.
Subject(s): Monoclonal antibodies
Sailfish
Billfishes--Identification
Billfishes
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/15052
Sublocation: Digital Library
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.