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Biochemical and biosynthetic optimization of bryostatin 1 in Bugula neritina

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Date Issued:
1993
Summary:
Bryostatin 1 is a macrocyclic lactone isolated from the marine bryozoan Bugula neritina. It is currently in clinical trials at the National Cancer Institute as a promising new antileukemic agent. Bryostatin 1 is found in Bugula neritina in poor yields (10^-4% dry weight). The focus of this research was to develop the biochemical and biosynthetic techniques that could be utilized to optimize the production of bryostatin 1 in Bugula neritina through aquaculture. To that end, the basic biosynthetic pathway to bryostatin 1 in the marine bryozoan was evaluated by using radiolabeled precursors. Techniques for the incorporation of these precursors were also developed. Other experiments involved an analysis of the composition of a specific population of Bugula neritina in terms of its bryostatin and lipid content. A determination of which lipids are produced de novo and which are exogenous was also undertaken.
Title: Biochemical and biosynthetic optimization of bryostatin 1 in Bugula neritina.
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Name(s): Vicchiarelli, Richard
Florida Atlantic University, Degree Grantor
Kerr, Russell G., Thesis Advisor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 1993
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 89 p.
Language(s): English
Summary: Bryostatin 1 is a macrocyclic lactone isolated from the marine bryozoan Bugula neritina. It is currently in clinical trials at the National Cancer Institute as a promising new antileukemic agent. Bryostatin 1 is found in Bugula neritina in poor yields (10^-4% dry weight). The focus of this research was to develop the biochemical and biosynthetic techniques that could be utilized to optimize the production of bryostatin 1 in Bugula neritina through aquaculture. To that end, the basic biosynthetic pathway to bryostatin 1 in the marine bryozoan was evaluated by using radiolabeled precursors. Techniques for the incorporation of these precursors were also developed. Other experiments involved an analysis of the composition of a specific population of Bugula neritina in terms of its bryostatin and lipid content. A determination of which lipids are produced de novo and which are exogenous was also undertaken.
Identifier: 14931 (digitool), FADT14931 (IID), fau:11713 (fedora)
Note(s): Thesis (M.S.)--Florida Atlantic University, 1993.
Subject(s): Bryozoa
Aquaculture--Research
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/14931
Sublocation: Digital Library
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.