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Evaluation of the specificity and sensitivity of monoclonal antibodies produced against sailfish albumin

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Date Issued:
1992
Summary:
Monoclonal antibodies (MAbs) were produced against sailfish (Istiophorus platypterus) albumin and investigated for their potential use in species identification. Balb/c mice were immunized with albumin purified from sailfish serum. Hybridomas were screened by enzyme-linked immunosorbent assay (ELISA) for reactivity with the purified albumins of sailfish, blue marlin (Makaira nigricans) and white marlin (Tetrapturus albidus). MAbs from 18 different hybridomas exhibited activity against sailfish albumin. Sixteen MAbs showed cross-reactivity with the marlin species. Two MAbs displayed distinct specificity for sailfish albumin. One of these MAbs (M2D1) was conjugated to horseradish peroxidase to construct an ELISA for the identification of sailfish from serum. The assay correctly identified 18 sailfish from 36 billfish samples. The MAb-peroxidase conjugate was highly specific toward sailfish with no detectable reaction against the heterologous species.
Title: Evaluation of the specificity and sensitivity of monoclonal antibodies produced against sailfish albumin.
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Name(s): Rossi, Edmund Anthony.
Florida Atlantic University, Degree grantor
Hartmann, James X., Thesis advisor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 1992
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 58 p.
Language(s): English
Summary: Monoclonal antibodies (MAbs) were produced against sailfish (Istiophorus platypterus) albumin and investigated for their potential use in species identification. Balb/c mice were immunized with albumin purified from sailfish serum. Hybridomas were screened by enzyme-linked immunosorbent assay (ELISA) for reactivity with the purified albumins of sailfish, blue marlin (Makaira nigricans) and white marlin (Tetrapturus albidus). MAbs from 18 different hybridomas exhibited activity against sailfish albumin. Sixteen MAbs showed cross-reactivity with the marlin species. Two MAbs displayed distinct specificity for sailfish albumin. One of these MAbs (M2D1) was conjugated to horseradish peroxidase to construct an ELISA for the identification of sailfish from serum. The assay correctly identified 18 sailfish from 36 billfish samples. The MAb-peroxidase conjugate was highly specific toward sailfish with no detectable reaction against the heterologous species.
Identifier: 14801 (digitool), FADT14801 (IID), fau:11590 (fedora)
Collection: FAU Electronic Theses and Dissertations Collection
Note(s): Thesis (M.S.)--Florida Atlantic University, 1992.
Charles E. Schmidt College of Science
Subject(s): Sailfish
Monoclonal antibodies
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/14801
Sublocation: Digital Library
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.