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The establishment and study of two elasmobranch cell lines

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Date Issued:
1992
Summary:
The present study describes the first cell lines produced from members of class Chondrichthyes. Explants of brain tissue from Carcharhinus falciformis (silky shark) and Ginglymostoma cirratum (nurse shark) were incubated in a mammalian medium modified with the addition of urea, trimethylamine N-oxide, NaCl, and bovine serum. Primary monolayers were passaged with 0.025% trypsin in a modified saline solution. Silky shark cells grew optimally at 29C. The population doubling time for C. falciformis cells at passage 29 was 67 hours. For G. cirratum cells at passage 6 the population doubling was 84 hours. Silky shark cells grew over a broad range of osmolalities from 315 mOsm to a 1664 mOsm with optimal growth at 650 mOsm. A medium containing 10% dimethylsulfoxide allowed for cryopreservation with greater than 65% viability upon recovery. Current theories of elasmobranch osmoregulation are discussed in light of experimental data collected from studies conducted on the silky shark cell line.
Title: The establishment and study of two elasmobranch cell lines.
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Name(s): Poyer, James Christopher.
Florida Atlantic University, Degree grantor
Hartmann, James X., Thesis advisor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 1992
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 58 p.
Language(s): English
Summary: The present study describes the first cell lines produced from members of class Chondrichthyes. Explants of brain tissue from Carcharhinus falciformis (silky shark) and Ginglymostoma cirratum (nurse shark) were incubated in a mammalian medium modified with the addition of urea, trimethylamine N-oxide, NaCl, and bovine serum. Primary monolayers were passaged with 0.025% trypsin in a modified saline solution. Silky shark cells grew optimally at 29C. The population doubling time for C. falciformis cells at passage 29 was 67 hours. For G. cirratum cells at passage 6 the population doubling was 84 hours. Silky shark cells grew over a broad range of osmolalities from 315 mOsm to a 1664 mOsm with optimal growth at 650 mOsm. A medium containing 10% dimethylsulfoxide allowed for cryopreservation with greater than 65% viability upon recovery. Current theories of elasmobranch osmoregulation are discussed in light of experimental data collected from studies conducted on the silky shark cell line.
Identifier: 14795 (digitool), FADT14795 (IID), fau:11584 (fedora)
Note(s): Charles E. Schmidt College of Science
Thesis (M.S.)--Florida Atlantic University, 1992.
Subject(s): Sharks
Cell culture
Chondrichthyes
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/14795
Sublocation: Digital Library
Use and Reproduction: Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.