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Comparative study of shark (Carcharinus falciformis) and mammalian (Mesocricetus auratus) cells exposed to chemical carcinogens

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Date Issued:
1991
Summary:
It is not known by what mechanism members of the subclass Elasmobranchii derive their natural resistance to neoplasia. To determine if this resistance is at the cellular level, cultures of shark (Carcharinus falciformis) cells were exposed to varying concentrations of the chemical carcinogens methanesulfonic acid ethyl ester (EMS) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Treated cells were assayed for cytotoxicity, formation of transformed foci and growth in agarose. It was found that a greater chemical concentration was necessary to reach LC50 in shark cells than in mammalian cells (BHK21 Cl13). Shark cell cultures developed no transformed foci over a range of chemical concentrations of EMS whereas foci formed at all concentrations in BHK control cultures exposed to EMS. Treated shark cells did not develop transformed colonies in agarose, but the mammalian cells readily formed colonies. These results suggest that the natural resistance of elasmobranchs to neoplasia could be partially, if not entirely, regulated at the cellular level.
Title: Comparative study of shark (Carcharinus falciformis) and mammalian (Mesocricetus auratus) cells exposed to chemical carcinogens.
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Name(s): Ritchey, Donna Mae.
Florida Atlantic University, Degree grantor
Hartmann, James X., Thesis advisor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 1991
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 47 p.
Language(s): English
Summary: It is not known by what mechanism members of the subclass Elasmobranchii derive their natural resistance to neoplasia. To determine if this resistance is at the cellular level, cultures of shark (Carcharinus falciformis) cells were exposed to varying concentrations of the chemical carcinogens methanesulfonic acid ethyl ester (EMS) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Treated cells were assayed for cytotoxicity, formation of transformed foci and growth in agarose. It was found that a greater chemical concentration was necessary to reach LC50 in shark cells than in mammalian cells (BHK21 Cl13). Shark cell cultures developed no transformed foci over a range of chemical concentrations of EMS whereas foci formed at all concentrations in BHK control cultures exposed to EMS. Treated shark cells did not develop transformed colonies in agarose, but the mammalian cells readily formed colonies. These results suggest that the natural resistance of elasmobranchs to neoplasia could be partially, if not entirely, regulated at the cellular level.
Identifier: 14708 (digitool), FADT14708 (IID), fau:11499 (fedora)
Note(s): Charles E. Schmidt College of Science
Thesis (M.S.)--Florida Atlantic University, 1991.
Subject(s): Carcharhinus
Carcinogens
Golden hamster
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/14708
Sublocation: Digital Library
Use and Reproduction: Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.