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EVALUATION OF MICROBIOLOGICAL METHODS FOR USE IN MARINE WATERS: ENUMERATION OF STAPHYLOCOCCI BY A FLUORESCENT ANTIBODY TECHNIQUE, AND ISOTOPIC DETERMINATION OF BACTERIAL METABOLIC POTENTIAL

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Date Issued:
1973
Summary:
Two methods, a membrane filter-fluorescent antibody technique for detection of Staphylococcus aureus, and isotopic determination of relative heterotrophic activity, were compared with a standard technique based on the recovery of coliform organisms for evaluation as indices of marine pollution. Water samples were collected from coastal areas of Pompano Beach and Boca Raton, Florida. Coliform and staphylococci survival in seawater was studied under laboratory conditions. The effect of exposure to seawater on substrate uptake by coliforms and other sewage microorganisms was studied using isotopic methods. The results of these studies support the feasibility of using S· aureus as a long-range indicator of marine pollution. Under natural conditions, S· aureus appears more resistant to seawater than coliform organisms, although the reverse was found under laboratory conditions. Seawater inhibited uptake of glucose and lactose by Escherichia coli and other sewage organisms. Relative heterotrophic potentials in terms of glucose and lactose uptake velocities were not significantly affected in coastal waters by the influx of bacteria and nutrients in sewage outfall effluent.
Title: AN EVALUATION OF MICROBIOLOGICAL METHODS FOR USE IN MARINE WATERS: ENUMERATION OF STAPHYLOCOCCI BY A FLUORESCENT ANTIBODY TECHNIQUE, AND ISOTOPIC DETERMINATION OF BACTERIAL METABOLIC POTENTIAL.
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Name(s): BUCHANAN, IAN ELLIS.
Florida Atlantic University, Degree grantor
Hoffmann, H. A., Thesis advisor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 1973
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 51 p.
Language(s): English
Summary: Two methods, a membrane filter-fluorescent antibody technique for detection of Staphylococcus aureus, and isotopic determination of relative heterotrophic activity, were compared with a standard technique based on the recovery of coliform organisms for evaluation as indices of marine pollution. Water samples were collected from coastal areas of Pompano Beach and Boca Raton, Florida. Coliform and staphylococci survival in seawater was studied under laboratory conditions. The effect of exposure to seawater on substrate uptake by coliforms and other sewage microorganisms was studied using isotopic methods. The results of these studies support the feasibility of using S· aureus as a long-range indicator of marine pollution. Under natural conditions, S· aureus appears more resistant to seawater than coliform organisms, although the reverse was found under laboratory conditions. Seawater inhibited uptake of glucose and lactose by Escherichia coli and other sewage organisms. Relative heterotrophic potentials in terms of glucose and lactose uptake velocities were not significantly affected in coastal waters by the influx of bacteria and nutrients in sewage outfall effluent.
Identifier: 13557 (digitool), FADT13557 (IID), fau:10400 (fedora)
Collection: FAU Electronic Theses and Dissertations Collection
Note(s): Thesis (M.S.)--Florida Atlantic University, 1973.
Charles E. Schmidt College of Science
Subject(s): Staphylococcus aureus
Marine pollution
Sewage disposal in the ocean
Sewage--Microbiology
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/13557
Sublocation: Digital Library
Use and Reproduction: Copyright © is held by the author, with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.