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PRODUCTION, INDUCTION, AND RESOLUTION OF THE CELLULOLYTIC ENZYME COMPLEX IN SELECTED FILAMENTOUS MARINE FUNGI

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Date Issued:
1972
Summary:
Cellulolytic enzymes were induced in the marine fungal isolates, Culcitalna achraspora, Halosphaeria mediosetigera, and Zalerion xylestrix by native cotton and related substrates. Marine environmental conditions were maintained in an artificial seawater medium. Induced c1 and ex enzymes and cellobiase were determined colorimetrically. Induction also occurred on cellobiose, but not on glucose and lactose. Permenter studies with glucose and with toilet tissue carbon sources gave close correlations between mycelial dry weight, enzyme component activity, and cell nitrogen. In cotton substrate media, Tween 80 increased C1 activity by 148%, and Cx activity by 160%, on the average. Millipore ultrafiltration was the best technique for concentrating cellulase for Sephadex G-75 column chromatography, while conventional techniques were unsuccessful. Fractionation diagrams for all cultures were similar with 2 distinct peaks. The low molecular weight fraction contained mainly Cx enzyme activity, while the high molecular weight fraction contained C1 and Cx enzymes and cellobiase. These fungi possess a cellulase similar, but not identical, to that found in terrestrial species.
Title: PRODUCTION, INDUCTION, AND RESOLUTION OF THE CELLULOLYTIC ENZYME COMPLEX IN SELECTED FILAMENTOUS MARINE FUNGI.
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Name(s): WHITE, JAMES L., JR.
Florida Atlantic University, Degree Grantor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 1972
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 73 p.
Language(s): English
Summary: Cellulolytic enzymes were induced in the marine fungal isolates, Culcitalna achraspora, Halosphaeria mediosetigera, and Zalerion xylestrix by native cotton and related substrates. Marine environmental conditions were maintained in an artificial seawater medium. Induced c1 and ex enzymes and cellobiase were determined colorimetrically. Induction also occurred on cellobiose, but not on glucose and lactose. Permenter studies with glucose and with toilet tissue carbon sources gave close correlations between mycelial dry weight, enzyme component activity, and cell nitrogen. In cotton substrate media, Tween 80 increased C1 activity by 148%, and Cx activity by 160%, on the average. Millipore ultrafiltration was the best technique for concentrating cellulase for Sephadex G-75 column chromatography, while conventional techniques were unsuccessful. Fractionation diagrams for all cultures were similar with 2 distinct peaks. The low molecular weight fraction contained mainly Cx enzyme activity, while the high molecular weight fraction contained C1 and Cx enzymes and cellobiase. These fungi possess a cellulase similar, but not identical, to that found in terrestrial species.
Identifier: 13537 (digitool), FADT13537 (IID), fau:10381 (fedora)
Note(s): Thesis (M.S.)--Florida Atlantic University, 1972.
Subject(s): Marine fungi
Cellulose--Biodegradation
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/13537
Sublocation: Digital Library
Use and Reproduction: Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder.
Use and Reproduction: http://rightsstatements.org/vocab/InC/1.0/
Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.