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EVIDENCE FOR THE EXISTENCE OF THE TRICARBOXYLIC ACID CYCLE AND GLYOXYLATE PATHWAY IN CERTAIN MARINE FILAMENTOUS FUNGI

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Date Issued:
1972
Summary:
Cellulolytic marine isolates, Culcitalna achraspora and Halosphaeria mediosetigera, were shaker-grown at 25 C in artificial seawater containing glucose, NH4NO3 , tris(hydroxymethyl) aminomethane and yeast extract, pH 7.5. Cultures were harvested in the late linear phase where whole cell endogenous respiration was maximal. Spectrophotometric determinations on acetone powder extracts revealed the presence of aconitase, NAD-linked isocitrate dehydrogenase, succinate dehydrogenase, fumarase, malate dehydrogenase and isocitratase. Culcitalna also showed NADP-linked isocitrate dehydrogenase activity. Manometric examinations of acetone powder and dialyzed sonic extracts were largely confirmatory. Oxidation of citric acid cycle intermediates by extracts were observed with Culcitalna for all but succinate with sonic extracts and for all but succinate and 2-oxoglutarate in acetone powder extracts. With Halosphaeria, neither extract could oxidize citrate or succinate, while acetone powder extracts were unable to degrade cis-aconitate and 2-oxoglutarate. Native mycelia were active only against malate. Attempts to permeate cell walls with ethyl esters of intermediates failed.
Title: EVIDENCE FOR THE EXISTENCE OF THE TRICARBOXYLIC ACID CYCLE AND GLYOXYLATE PATHWAY IN CERTAIN MARINE FILAMENTOUS FUNGI.
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Name(s): VEMBU, DEVI T.
Florida Atlantic University, Degree Grantor
Type of Resource: text
Genre: Electronic Thesis Or Dissertation
Issuance: monographic
Date Issued: 1972
Publisher: Florida Atlantic University
Place of Publication: Boca Raton, Fla.
Physical Form: application/pdf
Extent: 94 p.
Language(s): English
Summary: Cellulolytic marine isolates, Culcitalna achraspora and Halosphaeria mediosetigera, were shaker-grown at 25 C in artificial seawater containing glucose, NH4NO3 , tris(hydroxymethyl) aminomethane and yeast extract, pH 7.5. Cultures were harvested in the late linear phase where whole cell endogenous respiration was maximal. Spectrophotometric determinations on acetone powder extracts revealed the presence of aconitase, NAD-linked isocitrate dehydrogenase, succinate dehydrogenase, fumarase, malate dehydrogenase and isocitratase. Culcitalna also showed NADP-linked isocitrate dehydrogenase activity. Manometric examinations of acetone powder and dialyzed sonic extracts were largely confirmatory. Oxidation of citric acid cycle intermediates by extracts were observed with Culcitalna for all but succinate with sonic extracts and for all but succinate and 2-oxoglutarate in acetone powder extracts. With Halosphaeria, neither extract could oxidize citrate or succinate, while acetone powder extracts were unable to degrade cis-aconitate and 2-oxoglutarate. Native mycelia were active only against malate. Attempts to permeate cell walls with ethyl esters of intermediates failed.
Identifier: 13478 (digitool), FADT13478 (IID), fau:10324 (fedora)
Note(s): Thesis (M.S.)--Florida Atlantic University, 1972.
Subject(s): Marine fungi
Held by: Florida Atlantic University Libraries
Persistent Link to This Record: http://purl.flvc.org/fcla/dt/13478
Sublocation: Digital Library
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Host Institution: FAU
Is Part of Series: Florida Atlantic University Digital Library Collections.