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EFFECT OF TEMPERATURE, HYDROGEN ION AND CERTAIN GROWTH SUBSTRATES ON THE ACTIVITY OF THE CELLULOLYTIC ENZYME COMPLEX IN SELECTED MARINE FUNGI
| Title: | THE EFFECT OF TEMPERATURE, HYDROGEN ION AND CERTAIN GROWTH SUBSTRATES ON THE ACTIVITY OF THE CELLULOLYTIC ENZYME COMPLEX IN SELECTED MARINE FUNGI. |
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| Name(s): |
JENSEN, JAMES ROBERT Florida Atlantic University, Degree grantor Sguros, Peter L., Thesis advisor Charles E. Schmidt College of Science Department of Biological Sciences |
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| Type of Resource: | text | |
| Genre: | Electronic Thesis Or Dissertation | |
| Issuance: | monographic | |
| Date Issued: | 1971 | |
| Publisher: | Florida Atlantic University | |
| Place of Publication: | Boca Raton, FL | |
| Physical Form: | application/pdf | |
| Extent: | 100 p. | |
| Language(s): | English | |
| Summary: | Halosphaeria mediosetigera and Culcitalna achraspora have been studied to elucidate the mechanism by which they degrade cellulosic materials in the sea. H. mediosetigera (700 LC 1), a C1-less variant, was also employed in this work. Standardized shake-cultures were grown on cellulose and cellulose derivatives supplemented with NH4N03, tris(hydroxymethyl)aminomethane and yeast extract in artificial sea water (Lyman and Fleming), pH 7.5, at 25 C. Induced C1 and Cx enzyme and cellobiase activities were determined calorimetrically by correlation with cotton fiber weight loss, by the formation of reducing sugars from carboxymethylcellulose and by Glucostat, respectively. Optimum pH values for both Cx enzyme and cellobiase of C. achraspora were 6.0 at 37 C while those for H. mediosetigera (700 LC 1) were 5.0 and 6.0, respectively, at 37 C. In both cultures, Cx enzymes had a variable optimum temperature ( 37-50 C) at pH 6.0, depending on the length of the incubation period, while cellobiases had the same optimum temperature (50 C) at pH 6.0. Thermostability observations on Cx enzyme and cellobiase of C. achraspora showed almost complete deactivation at 100 C in 15 min for the former and 50 C in 15 min for the latter. Thermostability observations on Cx enzyme and cellobiase of H. mediosetigera (700 LC 1) showed almost complete deactivation at 45 C in 15 min for the former and at 50 C in 15 min for the latter. Data indicate that at least three enzymatic functions are involved in cellulose breakdown by these cultures. | |
| Identifier: | 13434 (digitool), FADT13434 (IID), fau:10283 (fedora) | |
| Degree granted: | Thesis (M.S.)--Florida Atlantic University, 1971. | |
| Collection: | FAU Electronic Theses and Dissertations Collection | |
| Note(s): | Charles E. Schmidt College of Science | |
| Subject(s): |
Marine fungi Cellulose--Biodegradation |
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| Held by: | Florida Atlantic University Libraries | |
| Persistent Link to This Record: | http://purl.flvc.org/fcla/dt/13434 | |
| Sublocation: | Digital Library | |
| Use and Reproduction: | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
| Use and Reproduction: | http://rightsstatements.org/vocab/InC/1.0/ | |
| Host Institution: | FAU | |
| Is Part of Series: | Florida Atlantic University Digital Library Collections. |
