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Posttranscriptional regulation of tropomyosin expression by myofibril inducing RNA (MIR) during axolotl embryonic heart development
| Title: | Posttranscriptional regulation of tropomyosin expression by myofibril inducing RNA (MIR) during axolotl embryonic heart development. |
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| Name(s): |
Jia, Pingping Florida Atlantic University, Degree grantor Lemanski, Larry F., Thesis advisor Charles E. Schmidt College of Medicine Department of Biomedical Science |
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| Type of Resource: | text | |
| Genre: | Electronic Thesis Or Dissertation | |
| Issuance: | monographic | |
| Date Issued: | 2006 | |
| Publisher: | Florida Atlantic University | |
| Place of Publication: | Boca Raton, FL | |
| Physical Form: | application/pdf | |
| Extent: | 47 p. | |
| Language(s): | English | |
| Summary: | A naturally-occurring recessive lethal mutation in axolotls, Ambystoma mexicanum, is an intriguing model for studying tropomyosin expression regulation. Homozygous embryos(c/c) form hearts that are deficient in tropomyosin, lack organized myofibrils and fail to beat. Previous studies have shown that a non-coding RNA gene, MIR (Myofibril Inducing RNA), is sufficient to rescue the non-beating homozygous recessive mutant hearts by promoting sarcomeric tropomyosin expression that leads to formation of organized myofibrils and beating hearts. Real time RT-PCR reveals that mutant hearts express the same level mRNA of the alpha-tropomyosin and TM4 type tropomyosin (ATmC-3) gene as normal embryonic hearts. These genes show no differences with regard to the splicing patterns of normal and mutant. Using protease inhibitor LLnL and E-64d treatments and two-dimensional Western blots of normal and mutant hearts, it is found that mutant hearts express all tropomyosin protein isoforms as normal hearts but protein expression are at low levels. These studies suggest that there is a failure in the translational or posttranslational control mechanisms for tropomyosin protein synthesis in cardiac mutant axolotl hearts during development. | |
| Identifier: | 9780542745904 (isbn), 13380 (digitool), FADT13380 (IID), fau:10230 (fedora) | |
| Degree granted: | Thesis (M.S.)--Florida Atlantic University, 2006. | |
| Collection: | FAU Electronic Theses and Dissertations Collection | |
| Note(s): | Charles E. Schmidt College of Science | |
| Subject(s): |
Medical genetics Molecular biology Cell differentiation Gene expression Axolotls--Development Heart--Growth--Molecular aspects |
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| Held by: | Florida Atlantic University Libraries | |
| Persistent Link to This Record: | http://purl.flvc.org/fcla/dt/13380 | |
| Sublocation: | Digital Library | |
| Use and Reproduction: | Copyright © is held by the author with permission granted to Florida Atlantic University to digitize, archive and distribute this item for non-profit research and educational purposes. Any reuse of this item in excess of fair use or other copyright exemptions requires permission of the copyright holder. | |
| Use and Reproduction: | http://rightsstatements.org/vocab/InC/1.0/ | |
| Host Institution: | FAU | |
| Is Part of Series: | Florida Atlantic University Digital Library Collections. |
