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- Title
- ESTABLISHING A SPONGE HYBRIDOMA FOR THE IN VITRO PRODUCTION OF A MARINE NATURAL PRODUCT.
- Creator
- Dougan, Cassady, Pomponi, Shirley A., Florida Atlantic University, Department of Marine Science and Oceanography, Charles E. Schmidt College of Science
- Abstract/Description
-
Marine sponges are one of the most prolific sources of chemical compounds with pharmaceutical importance. To establish a supply of such compounds large enough for clinical development, in vitro production methodology was investigated. Since all sponge cells do not divide in culture, it was hypothesized that the fusion of rapidly dividing cells of a sponge that does not produce any compounds of interest with cells of a nondividing but compound-producing sponge would result in a hybridoma that...
Show moreMarine sponges are one of the most prolific sources of chemical compounds with pharmaceutical importance. To establish a supply of such compounds large enough for clinical development, in vitro production methodology was investigated. Since all sponge cells do not divide in culture, it was hypothesized that the fusion of rapidly dividing cells of a sponge that does not produce any compounds of interest with cells of a nondividing but compound-producing sponge would result in a hybridoma that produces the compound of interest. In this study, hybridomas have been produced with cells of two marine sponges, Axinella corrugata, which produces the antitumor compound stevensine, and Geodia neptuni, which divides rapidly in a nutrient medium optimized for sponge cell culture. Successful hybridization and subsequent cell division and in vitro stevensine production may unlock the potential for sustainable mass production of other sponge-derived compounds.
Show less - Date Issued
- 2022
- PURL
- http://purl.flvc.org/fau/fd/FA00014024
- Subject Headings
- Marine natural products, Sponges, Hybridomas, In vitro
- Format
- Document (PDF)
- Title
- EVALUATION OF 3-D CULTURE METHODS FOR MARINE SPONGES.
- Creator
- Urban-Gedamke, Elizabeth, Pomponi, Shirley A., Florida Atlantic University, Department of Marine Science and Oceanography, Charles E. Schmidt College of Science
- Abstract/Description
-
Marine sponges are economically and environmentally valuable, but restoration, commercial, and biomedical demands exceed what wild populations and aquaculture can provide. In vitro culture of sponge cells is a promising alternative, but has remained elusive until recent breakthroughs involving improved nutrient medium M1 in two-dimensional culture. The advantages of three-dimensional over two-dimensional cell culture have been increasingly recognized. Here we report the successful 21-day...
Show moreMarine sponges are economically and environmentally valuable, but restoration, commercial, and biomedical demands exceed what wild populations and aquaculture can provide. In vitro culture of sponge cells is a promising alternative, but has remained elusive until recent breakthroughs involving improved nutrient medium M1 in two-dimensional culture. The advantages of three-dimensional over two-dimensional cell culture have been increasingly recognized. Here we report the successful 21-day culture of cells from the marine sponge Geodia neptuni using multiple three-dimensional cell culture methods: FibraCel© disks, thin hydrogel layers, gel micro droplets, and spheroid cell culture. These methods performed comparably to two-dimensional control cultures, and each method offers advantages for restoration or in vitro applications using sponge cells. Further optimization of these methods may lead to the ability to culture fully functioning sponges from dissociated, cryopreserved cells, which will reduce the need for wild harvest of sponge tissues for commercial, restoration, and biomedical purposes.
Show less - Date Issued
- 2021
- PURL
- http://purl.flvc.org/fau/fd/FA00013726
- Subject Headings
- Sponges, Cell Culture--methods
- Format
- Document (PDF)
- Title
- Leiodermatolide, a potent antimitotic macrolide from the marine sponge Leiodermatium sp.
- Creator
- Paterson, Ian, Dalby, S. M., Roberts, J. C., Naylor, G. J., Guzman, Esther A., Isbrucker, Richard A., Pitts, Tara P., Linley, P. A., Divlianska, D. B., Reed, John K., Wright, Amy E.
- Date Issued
- 2011
- PURL
- http://purl.flvc.org/fau/fd/FA00007465
- Subject Headings
- Antimitotic Agents, Macrolides, Sponges, Molecular structure, Nuclear Magnetic Resonance, Biomolecular, Cell lines
- Format
- Document (PDF)
- Title
- Sponge cell culture.
- Creator
- Pomponi, Shirley A., Willoughby, Robin, Kelly-Borges, M.
- Date Issued
- 1998
- PURL
- http://purl.flvc.org/fau/fd/FA00007453
- Subject Headings
- Sponges, Sponges--Cytology, Cell culture
- Format
- Document (PDF)
- Title
- A molecular systematic survey of sponge derived microbes.
- Creator
- Sandell, K., Peterson, C. L., Harmody, Dedra K., McCarthy, Peter J., Pomponi, Shirley A., Lopez, Jose V.
- Date Issued
- 2004
- PURL
- http://purl.flvc.org/fau/fd/FA00007425
- Subject Headings
- Sponges, Marine microbiology, Symbiosis, Polymorphism, Restriction Fragment Length, DNA Fingerprinting
- Format
- Document (PDF)
- Title
- Sponge cell culture for production of bioactive metabolites.
- Creator
- Pomponi, Shirley A., Willoughby, Robin
- Date Issued
- 1994
- PURL
- http://purl.flvc.org/fau/fd/FA00007381
- Subject Headings
- Sponges, Cell culture, Metabolites, Bioactive compounds
- Format
- Document (PDF)
- Title
- A new bicyclic guanidine alkaloid, Sch 575948, from amarine sponge, Ptilocaulis spiculifer.
- Creator
- Yang, Shu-Wei, Chan, Tze-Ming, Pomponi, Shirley A., Chen, Guodong, Wright, Amy E., Patel, Mahesh, Gullo, Vincent, Pramanik, B., Chu, Min
- Date Issued
- 2003
- PURL
- http://purl.flvc.org/fau/fd/FA00007361
- Subject Headings
- Sponges, Alkaloids, Guanidines, Antibacterial agents, Molecular structure
- Format
- Document (PDF)
- Title
- Cytotoxic peptides from marine sponges.
- Creator
- Gulavita, N. K., Wright, Amy E., McCarthy, Peter J., Pomponi, Shirley A., Longley, Ross E.
- Date Issued
- 1996
- PURL
- http://purl.flvc.org/fau/fd/FA00007341
- Subject Headings
- Sponges, Peptides, Cytotoxins, Stereochemistry
- Format
- Document (PDF)
- Title
- A new deep sea coralline sponge from Turks and Caicos Islands: Willardi caicosensis gen. et sp. nov.(Demospongiae: Hadromerida).
- Creator
- Willenz, P., Pomponi, Shirley A.
- Date Issued
- 1996
- PURL
- http://purl.flvc.org/fau/fd/FA00007343
- Subject Headings
- Turks and Caicos Islands, Atlantic Ocean, Sponges, Demospongiae, Coralline, Species, Genus
- Format
- Document (PDF)
- Title
- Development of techniques for in vitro production of bioactive natural products from marine sponges.
- Creator
- Pomponi, Shirley A., Willoughby, Robin, Kaighn, M. E., Wright, Amy E.
- Date Issued
- 1997
- PURL
- http://purl.flvc.org/fau/fd/FA00007344
- Subject Headings
- Sponges, Bioactive compounds, Marine natural products, Cell culture, Growth factors, Phytohemagglutinins, Cryopreservation
- Format
- Document (PDF)
- Title
- The chemistry and biological activity of five marine sponges of the genus Aplysina.
- Creator
- Gunasekera, Sarath P., Cross, S. S., Longley, Ross E., Pomponi, Shirley A.
- Date Issued
- 1991
- PURL
- http://purl.flvc.org/fau/fd/FA00007303
- Subject Headings
- Sponges, Antiviral agents, Immunosuppressive agents, Bioactive compounds, Marine natural products
- Format
- Document (PDF)
- Title
- The discovery and characterization of an interleukin 6 cytokine familyantagonist protein from a marine sponge, Callyspongia sp.
- Creator
- Peppard, J. V., Loo, P., Sills, Matthew A., Wennogle, L., Wright, Amy E., Pomponi, Shirley A., Cueto, M.
- Date Issued
- 2001
- PURL
- http://purl.flvc.org/fau/fd/FA00007313
- Subject Headings
- Sponges, Interleukin-6, Cytokines--antagonists & inhibitors, Proteins--chemistry
- Format
- Document (PDF)
- Title
- Eryloside E from an Atlantic sponge Erylus goffrilleri.
- Creator
- Gulavita, N. K., Wright, Amy E., Kelly-Borges, M., Longley, Ross E., Yarwood, Donna, Sills, Matthew A.
- Date Issued
- 1994
- PURL
- http://purl.flvc.org/fau/fd/FA00007261
- Subject Headings
- Sponges, Terpenes, Glycosides, Chemical structure
- Format
- Document (PDF)
- Title
- Characterization of an interleukin 6 cytokinefamily antagonist protein from a marine sponge, Callyspongia sp.
- Creator
- Peppard, J. V., Loo, P., Sills, Matthew A., Munster, D., Pomponi, Shirley A., Wright, Amy E.
- Date Issued
- 1996
- PURL
- http://purl.flvc.org/fau/fd/FA00007266
- Subject Headings
- Sponges, Interleukin-6, Cytokines--antagonists & inhibitors
- Format
- Document (PDF)
- Title
- Spongionella foliascens: a new foliose dendroceratid sponge from the western Atlantic.
- Creator
- Kelly-Borges, M., Pomponi, Shirley A., Vacelet, J.
- Date Issued
- 1993
- PURL
- http://purl.flvc.org/fau/fd/FA00007223
- Subject Headings
- Mexico, Gulf of, Porifera, Sponges, Taxonomy, Animal, Species
- Format
- Document (PDF)
- Title
- 1,1-Dimethyl-5,6-dihydroxyindolinium chloride from a deep water marine sponge, Dercitus sp.
- Creator
- Kohmoto, S., McConnell, O. J., Wright, Amy E.
- Date Issued
- 1988
- PURL
- http://purl.flvc.org/fau/fd/FA00007184
- Subject Headings
- Sponges, Marine metabolites, Chemical structure
- Format
- Document (PDF)
- Title
- Tsukamurella spongiae sp. nov., a novel actinomycete isolated from a deep-water marinesponge.
- Creator
- Olson, Julie B., Harmody, Dedra K., Bej, A. K., McCarthy, Peter J.
- Date Issued
- 2007
- PURL
- http://purl.flvc.org/fau/fd/FA00007117
- Subject Headings
- Curaçao, Sponges, Actinobacteria, Actinomycetes, RNA, Ribosomal, 16S
- Format
- Document (PDF)
- Title
- Stereochemical determination of dictyostatin, a novel microtubule-stabilising macrolide from the marine sponge Corallistidae sp.
- Creator
- Paterson, Ian, Britton, R., Delgado, Oscar, Wright, Amy E.
- Date Issued
- 2004
- PURL
- http://purl.flvc.org/fau/fd/FA00007061
- Subject Headings
- Macrolides, Sponges, Tubulin Modulators, Stereochemistry, Microtubules, Polyketides, Antimitotic Agents
- Format
- Document (PDF)
- Title
- Total synthesis of a potent hybrid of the anticancer natural products dictyostatin and discodermolide.
- Creator
- Paterson, Ian, Naylor, G. J., Wright, Amy E.
- Date Issued
- 2008
- PURL
- http://purl.flvc.org/fau/fd/FA00007062
- Subject Headings
- Antineoplastic agents, Macrolides, Polyketides, Tubulin Modulators, Marine natural products, Sponges
- Format
- Document (PDF)
- Title
- Early effects of Lasonolide A on pancreatic cancer cells.
- Creator
- Isbrucker, Richard A., Guzman, Esther A., Pitts, Tara P., Wright, Amy E.
- Date Issued
- 2000
- PURL
- http://purl.flvc.org/fau/fd/FA00007053
- Subject Headings
- Macrolides, Sponges, Marine natural products, Pancreatic Neoplasms, Pancreas--Cancer
- Format
- Document (PDF)