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Title: 2D AND 3D SHAPE VARIATION AMONG ELASMOBRANCH OLFACTORY ROSETTES.
Creator: Clark, Aubrey E., Porter, Marianne E., Meredith, Tricia L., Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: The functional impacts of olfactory rosette variation in elasmobranchs is unresolved. Our goal was to quantify rosette morphology and shape from 14 species using dissections, phylogenetic comparisons, and microCT imaging. We hypothesized that lamellar count and rosette shape (fineness ratio) would not scale with animal size, but internal rosette size variables must scale positively. We found that fineness ratio and lamellar counts varied significantly among species, and were positively...
Show moreThe functional impacts of olfactory rosette variation in elasmobranchs is unresolved. Our goal was to quantify rosette morphology and shape from 14 species using dissections, phylogenetic comparisons, and microCT imaging. We hypothesized that lamellar count and rosette shape (fineness ratio) would not scale with animal size, but internal rosette size variables must scale positively. We found that fineness ratio and lamellar counts varied significantly among species, and were positively correlated. The first two principal components of the pPCA explained 82% of the variation, with fineness ratio and lamellar count contributing the most. There were no significant differences between rosette structure or volume when comparing dissected values to in situ values obtained using diceCT. Based on our results, we hypothesize that variations in rosette shape and morphology will impact hydrodynamics and optimize odorant detection, and these data can be used to create 3D models for future hydrodynamic studies.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013591
Subject Headings: Elasmobranchs, Olfactory sensors, Morphology
Format: Document (PDF)

Title: THE EFFECTS OF WILLOW SHRUB ENCROACHMENT ON SOIL ORGANIC CARBON STORAGE IN A SOUTH FLORIDA HERBACEOUS WETLAND.
Creator: Dell, Jessica Ann, Benscoter, Brian, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: Storing almost a third of the global soil carbon pool, wetlands are an essential component of the carbon cycle, and carbon-rich peat soil accumulates when carbon input through primary productivity exceeds output through decomposition. However, woody shrub encroachment in herbaceous wetlands can alter soil carbon processes, potentially diminishing stored carbon. To examine the effects of shrub encroachment on soil carbon, I compared soil carbon input through litterfall and fine root production...
Show moreStoring almost a third of the global soil carbon pool, wetlands are an essential component of the carbon cycle, and carbon-rich peat soil accumulates when carbon input through primary productivity exceeds output through decomposition. However, woody shrub encroachment in herbaceous wetlands can alter soil carbon processes, potentially diminishing stored carbon. To examine the effects of shrub encroachment on soil carbon, I compared soil carbon input through litterfall and fine root production, output through decomposition, and below-canopy microclimate conditions between Carolina willow shrub (Salix caroliniana) and herbaceous sawgrass (Cladium jamaicense) in the Blue Cypress Marsh Conservation Area (BCMCA), FL. To assess the level of production and its response to water level, I compared aboveground green biomass by measuring normalized difference vegetation index (NDVI) and photosynthetic stress by measuring photochemical reflectance index (PRI) between sawgrass and willow. I collected willow litterfall using litter traps and measured sawgrass and willow fine root production with fine root ingrowth bags. Litter decomposition was measured with decomposition bags deployed using a reciprocal litter placement design at BCMCA and incubated in a greenhouse to examine the effects of char and water level on decomposition. Above and belowground microclimate conditions were measured using sensors installed within sawgrass and willow canopies. Despite experiencing more photosynthetic stress, willow produced more green biomass than sawgrass. However, willow produced fewer fine roots than sawgrass and these roots were deeper within the soil. Willow litter decomposed faster even though sawgrass decomposition increased under drier conditions. Compared to the sawgrass canopy, the willow canopy had greater light availability, lower evaporative demand plus warmer and drier soils; however, litter decomposition did not differ between the canopies. These results suggest that willow encroachment can reduce the amount and alter the distribution of carbon within an herbaceous wetland, likely resulting in a net loss of soil carbon. Although willow encroachment may increase aboveground biomass carbon stocks, these stocks will likely be offset by a loss of soil carbon due to reduced fine root production and increased decomposition. Therefore, the transition from herbaceous wetland to shrub wetland will likely result in a loss of stored soil carbon.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013621
Subject Headings: Wetlands, Blue Cypress Water Management Area (Fla), Carbon cycle (Biogeochemistry), Soils
Format: Document (PDF)

Title: POPULATION LIMITATIONS OF THE FLORIDA APPLE SNAIL (POMACEA PALUDOSA).
Creator: Drumheller, Danielle, Dorn, Nathan, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: In this thesis I examined factors limiting population success of apple snails (Pomacea spp.) in Florida wetlands. First, I examined effects of summertime hydropattern in replicated wetlands on reproduction and juvenile growth. Annual reproductive effort of Florida apple snails (Pomacea paludosa) and the invasive apple snail P. maculata under was not affected by deeper water in the summer. When juvenile P. paludosa were forced to grow in reportedly favorable and relatively deeper summertime...
Show moreIn this thesis I examined factors limiting population success of apple snails (Pomacea spp.) in Florida wetlands. First, I examined effects of summertime hydropattern in replicated wetlands on reproduction and juvenile growth. Annual reproductive effort of Florida apple snails (Pomacea paludosa) and the invasive apple snail P. maculata under was not affected by deeper water in the summer. When juvenile P. paludosa were forced to grow in reportedly favorable and relatively deeper summertime depths survival was high between treatments and growth was unaffected. In the other chapter I examined interactions between the two snails with a series of observations and experiments. I examined historical data on the assemblages and found that P. paludosa were locally extinguished in one of the wetlands within six years of invasion by P. maculata. Two field experiments gave conflicting results about the importance of interspecific inhibition of P. paludosa by the invasive snail, but in either case resource reduction could not be demonstrated. Lab exposure to P. maculata chemical cues strongly inhibited P. paludosa growth, although P. paludosa did not behaviorally avoid mucus of P. maculata. In mesocosms Pomacea paludosa growth was reduced with increasing exposure to adult P. maculata waterborne cues, and the results suggest that contact with mucus at a low exposure may even have a greater inhibiting effect. This interspecific direct chemical growth inhibition was novel for gastropods, but could be more generally important, and its potential impacts to P. paludosa populations should be explored further.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013593
Subject Headings: Florida applesnail, Pomacea paludosa, Snail populations
Format: Document (PDF)

Title: GENETIC ANALYSIS AND EVOLUTIONARY HISTORY OF THE LOMAMI RIVER BLUE MONKEY, CERCOPITHECUS MITIS HEYMANSI, IN THE CENTRAL CONGO BASIN.
Creator: Larkin-Gero, Emma, Detwiler, Kate M., Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: Cercopithecus mitis is a polytypic, pan-African guenon species with a complex evolutionary history. The Lomami River Blue Monkey, C. m. heymansi, occurs within the Tshuapa-Lomami-Lualaba (TL2) Conservation Landscape and exhibits high pelage variation. Previous studies have shown discordance between the mitochondrial and Ychromosome gene trees of C. m. heymansi. The objectives were to test the phylogenetic pattern found in the mtDNA tree using a larger sample size of C. m. heymansi, determine...
Show moreCercopithecus mitis is a polytypic, pan-African guenon species with a complex evolutionary history. The Lomami River Blue Monkey, C. m. heymansi, occurs within the Tshuapa-Lomami-Lualaba (TL2) Conservation Landscape and exhibits high pelage variation. Previous studies have shown discordance between the mitochondrial and Ychromosome gene trees of C. m. heymansi. The objectives were to test the phylogenetic pattern found in the mtDNA tree using a larger sample size of C. m. heymansi, determine the genetic diversity of the population, and hypothesize methods of speciation. While many species within the C. mitis radiation depict paraphyly, I determined that C. m. heymansi forms highly supported monophyletic clades in both mtDNA and Ychromosome gene trees. These results suggest that C. m. heymansi is a distinct lineage within the C. mitis radiation. Future genomic analyses are important in resolving the evolutionary history of this population and discerning its taxonomic classification and conservation value.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013609
Subject Headings: Blue monkey, Cercopithecus mitis, Congo River
Format: Document (PDF)

Title: PROTANDRIC SEX CHANGE IN CENTROPOMUS UNDECIMALIS, THE COMMON SNOOK.
Creator: McKenna, Brent, Baldwin, John, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: Otolith increment morphology of the protandric hermaphrodite Centropomus undecimalis (snook) was analyzed in relation to age and length for sex specific growth differences to retroactively determine the time of sex change. Growth spurts in the otoliths of female snook, the terminal sex, were identified between ages 4 and 8, corroborating the current understanding of when sex change occurs (between 3 and 8). No such growth increases were identified on the otoliths of male snook, the primary...
Show moreOtolith increment morphology of the protandric hermaphrodite Centropomus undecimalis (snook) was analyzed in relation to age and length for sex specific growth differences to retroactively determine the time of sex change. Growth spurts in the otoliths of female snook, the terminal sex, were identified between ages 4 and 8, corroborating the current understanding of when sex change occurs (between 3 and 8). No such growth increases were identified on the otoliths of male snook, the primary sex. Otolith growth, however, was found to decouple from length, so these growth spurts are not reflected in the length of the fish. This study marks the first time that a distinct growth pattern differentiating the primary and terminal sexes, similar to that seen in the protogynous transitional growth spurt hypothesis, has been identified on the otoliths of a protandric species.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013622
Subject Headings: Snook, Sex change
Format: Document (PDF)

Title: THE TRIFECTA: A NOVEL COMBINATORIAL THERAPY SPARES IMMUNE CELLS WHILE INDUCING IMMUNOGENIC CELL DEATH IN HUMAN MAMMARY ADENOCARCINOMA AND MOUSE MAMMARY CARCINOMA.
Creator: Motii, Youssef, Hartmann, James X., Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: According to U.S. Breast Cancer Statistics, about 1 in 8 U.S. women will develop invasive breast cancer during their lifetime. Chemotherapeutics that are used on patients currently often lead to tumor resistance, bone marrow suppression and cachexia. This study evaluated a novel combination of three non-mutagenic compounds for their effectiveness against mammary tumor cells, toxicity towards immune cells, ability to provoke the expression of immunogenic cell death (ICD) markers, and killing...
Show moreAccording to U.S. Breast Cancer Statistics, about 1 in 8 U.S. women will develop invasive breast cancer during their lifetime. Chemotherapeutics that are used on patients currently often lead to tumor resistance, bone marrow suppression and cachexia. This study evaluated a novel combination of three non-mutagenic compounds for their effectiveness against mammary tumor cells, toxicity towards immune cells, ability to provoke the expression of immunogenic cell death (ICD) markers, and killing in 3D tumor models. Methotrexate (MTX), 2-deoxyglucose (2DG), and wogonin (WGN) were combined at doses well below their EC50 values yet effectively killed human and mouse breast cancer cells. The combination inhibited cancer cell colony formation and induced a high degree of cell death in multiple malignant tumor cell lines. Importantly, the combination did not significantly inhibit the viability of peripheral-blood mononuclear cells (PBMCs), even when employed at 3X the concentration that killed cancer cells. In marked contrast, low-dose doxorubicin, a common therapeutic for breast cancers, significantly decreased PBMC viability and increased the percentage of cell death. Our novel combinatorial therapy (Trifecta) elicited the significant expression of three ICD hallmarks: calreticulin surface expression, ATP secretion, and HMGB-1 release. In all cases, Trifecta elicited an equal or greater degree of ICD-marker expression compared to doxorubicin, a known inducer of ICD. We show significant efficacy of Trifecta against human and mouse mammary 3D tumor models grown in Matrigel® ECM-complex containing culture medium, and reaffirm the marked resistance of tumorspheres towards the conventional chemotherapeutic doxorubicin. The effectiveness of Trifecta in an acceptable surrogate model for mouse studies bodes well for translation of our findings to the clinic. In conclusion, Trifecta has proven highly effective against tumor cells grown either as monolayers or tumorspheres, without significant cytotoxic effects towards proliferating immune cells. Furthermore, treatment with this combination elicits ICD, which has the potential to prime an adaptive immune response against tumor cells and prevent future relapse. The drugs chosen for our combination target metabolic pathways that cancer cells are heavily dependent upon and do not interact with or induce mutations in DNA. These properties place Trifecta at the forefront of developing anticancer therapies.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013606
Subject Headings: Cancer--Treatment, Breast--Cancer, Methotrexate, Deoxyglucose, wogonin
Format: Document (PDF)

Title: ADAPTIVE EVOLUTION OF SLEEP LOSS AND ALBINISM THROUGH MUTATIONS IN OCA2 IN ASTYANAX MEXICANUS.
Creator: O'Gorman, Morgan, Keene, Alex, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: Adaptation to any environment through the course of its evolution involves the alteration of multiple traits to ensure survival, Astyanax mexicanus is a notable example of this. Some of the most notable differences between the surface and cave populations are loss of pigmentation, decreased sleep, and reduced eyes. 208 surface – cave F2 hybrids were assessed for several morphological and behavioral phenotypes. There were significant differences found between pigmented and albino individuals,...
Show moreAdaptation to any environment through the course of its evolution involves the alteration of multiple traits to ensure survival, Astyanax mexicanus is a notable example of this. Some of the most notable differences between the surface and cave populations are loss of pigmentation, decreased sleep, and reduced eyes. 208 surface – cave F2 hybrids were assessed for several morphological and behavioral phenotypes. There were significant differences found between pigmented and albino individuals, raising the question of a shared genetic basis for these traits. Oculocutaneous albinism 2 (oca2) is a notable contributor to albinism in many species, including A. mexicanus. Surface fish with CRISPR mutations at oca2 confirmed a contribution of oca2 in sleep loss within albino cavefish. Further, it was determined that oca2 is under selection and possibly has a pleiotropic function, which underlies the adaptive evolution of sleep loss and albinism.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013619
Subject Headings: Astyanax mexicanus, Astyanax mexicanus--Evolution, Oculocutaneous albinism type 2
Format: Document (PDF)

Title: STONY CORAL TISSUE LOSS DISEASE INTERVENTION STRATEGIES FOR MONTASTRAEA CAVERNOSA.
Creator: Shilling, Erin N., Voss, Joshua D., Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: Stony coral tissue loss disease (SCTLD) has been spreading throughout Caribbean coral reefs since 2014, heavily impacting scleractinian corals. To mitigate losses, this experiment assessed the effectiveness of two SCTLD intervention treatments in situ. SCTLD-affected Montastraea cavernosa colonies offshore of Broward County were divided into three treatment groups: 1) chlorinated epoxy, 2) Base 2B plus amoxicillin, and 3) untreated controls, plus a fourth group of healthy untreated controls....
Show moreStony coral tissue loss disease (SCTLD) has been spreading throughout Caribbean coral reefs since 2014, heavily impacting scleractinian corals. To mitigate losses, this experiment assessed the effectiveness of two SCTLD intervention treatments in situ. SCTLD-affected Montastraea cavernosa colonies offshore of Broward County were divided into three treatment groups: 1) chlorinated epoxy, 2) Base 2B plus amoxicillin, and 3) untreated controls, plus a fourth group of healthy untreated controls. These colonies were monitored over 11 months to record SCTLD status, lesion activity, colony mortality, and changes in healthy tissue area with 3D modelling. The Base 2B plus amoxicillin treatment was more effective at halting SCTLD lesions, slowing the rate of tissue loss, and decreasing the overall proportion of tissue lost as compared to chlorinated epoxy or no treatment. However, neither treatment prevented new SCTLD lesions from developing. These findings can enhance the effectiveness of Caribbean management efforts to mitigate SCTLD impacts.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013616
Subject Headings: Stony corals, Montastraea, Corals--Diseases
Format: Document (PDF)

Title: THE CRITICAL ROLE OF INTERACTIONS BETWEEN ECOLOGICAL FOUNDATION SPECIES IN STRUCTURING A MANGROVE COMMUNITY.
Creator: Aquino-Thomas, Jessene, Brooks, W. Randy, Proffitt, C. Edward, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: The main objective of this research was to analyze how well my proposed Foundation Species Interaction model explained the benthic communities found on red mangrove (Rhizophora mangle) prop roots. This research investigated the connections between the primary foundation species (mangroves), the dominant secondary foundations species (oysters, sponges, and barnacles), and the resulting biodiversity in order to understand the temporal and spatial variability of the ecosystems at different...
Show moreThe main objective of this research was to analyze how well my proposed Foundation Species Interaction model explained the benthic communities found on red mangrove (Rhizophora mangle) prop roots. This research investigated the connections between the primary foundation species (mangroves), the dominant secondary foundations species (oysters, sponges, and barnacles), and the resulting biodiversity in order to understand the temporal and spatial variability of the ecosystems at different community levels. Chapter 1 was dedicated to explaining my change in ecological theory, the Foundation Species Interaction model. The interactions included in the model between the foundations species that were examined were the mutualistic (+, +), commensal (+, 0), and parasitic (+, -) interactions. Chapter 2 focused on exploration of the mangrove ecosystem in Southeast Florida and establishing where the secondary foundation species and prop root epibionts where found along the latitudinal gradient. The survey investigated the connections between mangroves, the dominant secondary foundations species (e.g. oysters, sponges, and barnacles), and the resulting biodiversity to understand the temporal and spatial variability of the ecosystems at different community levels. Chapter 3 was dedicated to testing the Foundation Species Interaction model’s ability to predict the biodiversity along the latitudinal gradient of the survey. The difference in mangrove prop root communities were largely explained by where the communities laid along the latitudinal gradient and by secondary foundation species presence. The shift from one foundation species to another had sizable effects on biodiversity. Chapter 4 combined the Foundation Species Interaction model with the established predation hypothesis and tested their abilities to explain biodiversity along the latitudinal gradient. This allowed for shifts in community structure to be examined for top-down and bottom-up influences. Predation effects changed along the latitudinal gradient, as the predation effects changed so did the effects of each of the individual foundation species.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013561
Subject Headings: Mangrove ecology--Florida, Rhizophora mangle, Red mangrove--Ecology, Biodiversity & ecology
Format: Document (PDF)

Title: Call Categorization and Vocal Behavior of the Florida Manatee (Trichechus manatus latirostris).
Creator: Brady, Beth A., Moore, Jon, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: Florida manatees are semisocial marine mammals that vocalize when interacting with conspecifics and to maintain contact with offspring. While many aspects of their biology have been studied, there is a dearth of information on the diversity and complexity of vocal behavior during social, nonsocial, and stressful situations. Investigations of vocal communication repertoires which define, categorize, and correlate varied call types with behavior are needed in order to understand the behavioral...
Show moreFlorida manatees are semisocial marine mammals that vocalize when interacting with conspecifics and to maintain contact with offspring. While many aspects of their biology have been studied, there is a dearth of information on the diversity and complexity of vocal behavior during social, nonsocial, and stressful situations. Investigations of vocal communication repertoires which define, categorize, and correlate varied call types with behavior are needed in order to understand the behavioral and social function of associated calls. Arguably the most important social bond in manatees is the period of cow/calf dependency and empirical evidence indicates cows recognize the vocalizations of offspring. Exploration of individually distinctive vocal features can provide insight on which parameters might be salient to facilitate recognition between cows/calves. This study is focused on vocal communication in Florida manatees, how calls are structured, utilized and function while animals are distressed and during social interactions in their shallow water habitats. Hydrophones recorded vocalizations from individual calves and manatees in different behavioral contexts and varying size aggregations. Analysis of the vocal repertoire indicated manatee vocalizations can be parsed into five broadly defined call types which include the hill-shaped high squeak, tonal squeak, noisy squeal, two toned chirp, and the combinatorial squeak-squeal. Furthermore, the high squeak is likely a discrete call whereas the others are graded and do not have strict boundaries between call types (Chapter 2). Broadly defined call types were used to explore call usage with variations in behavior, group size, and group composition (Chapter 3). Manatees vocalized using few call types and altered structural parameters depending on behavioral state. Calls were longer and more frequency modulated when stressed. Vocalizations produced while cavorting were higher in entropy and more frequency modulated than when manatees were resting or feeding. Vocalizations obtained from individual calves suggest that the high squeak is a stereotypical call that is produced by smaller calves. All calves had individually distinctive acoustic features that could potentially be used in recognition (Chapter 4). Lower fundamental frequencies and higher emphasized frequencies from smaller calves suggest that the fundamental frequency may not be a reliable indicator of body size in calves. This research increases our knowledge of the vocal behavior and call characteristics of the Florida manatee.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013519
Subject Headings: Manatees, West Indian manatee--Florida, Trichechus manatus latirostris, Vocalization, Animal, Florida manatee
Format: Document (PDF)

Title: DEVELOPMENT OF AN ACELLULAR EXTRACELLULAR MATRIX AS A THREE-DIMENSIONAL SCAFFOLD FOR ESOPHAGEAL TUMOR ENGINEERING.
Creator: Chaitin, Hersh, Kang, Yunqing, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: Human esophageal squamous cell carcinoma (hESCC) is a very aggressive form of cancer due to its ability to easily metastasize into proximal lymph nodes and adjacent organs. The role of the extracellular matrix (ECM) and its stromal cells in metastasis remains unclear. To better understand the effect of the ECM and fibroblast cells on esophagus cancer cell migration and invasion, we propose a biomimetic human esophagus model cultured with hESCC and human primary fibroblast cells (fibroblast)....
Show moreHuman esophageal squamous cell carcinoma (hESCC) is a very aggressive form of cancer due to its ability to easily metastasize into proximal lymph nodes and adjacent organs. The role of the extracellular matrix (ECM) and its stromal cells in metastasis remains unclear. To better understand the effect of the ECM and fibroblast cells on esophagus cancer cell migration and invasion, we propose a biomimetic human esophagus model cultured with hESCC and human primary fibroblast cells (fibroblast). To mimic the extracellular matrix of human esophagus we use decellularized porcine esophagus matrix (DEM) to culture with hESCC and fibroblasts in static conditions. This DEM can recapitulate the human esophagus tumor microenvironment with relevant cues. This model will provide valuable information regarding esophagus cancer cell migration with respect to the heterogeneous extracellular matrix and stromal fibroblast cells. We expect to discover the mechanisms by which extracellular matrix and stromal cells affect cancer migration and invasion in vitro. Characterizing this process will provide vital insight towards the effects of fibroblasts cells on facilitating migration and invasion of esophageal cancer cells. This esophagus cancer model also provides promising potential to study drug screening and develop new strategies against esophagus metastasis.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013534
Subject Headings: Esophageal Squamous Cell Carcinoma, Extracellular matrix, Metastasis, Fibroblasts, Biomimetics, Stromal Cells
Format: Document (PDF)

Title: RESPONSES OF WOOD STORKS TO HUMAN-INDUCED LANDSCAPE CHANGE IN SOUTH FLORIDA.
Creator: Evans, Betsy A., Gawlik, Dale E., Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: There is a strong conservation need to understand traits of native species that adapt to urban environments, but results have been equivocal. Wetland birds have shown a strong phylogenetic signal towards urban tolerance; however, these species have largely been ignored in urban studies. I used Wood Storks (Mycteria americana) to determine how a wetland species of conservation concern responded to human-induced landscape change in South Florida. Specifically, my study investigated 1) resource...
Show moreThere is a strong conservation need to understand traits of native species that adapt to urban environments, but results have been equivocal. Wetland birds have shown a strong phylogenetic signal towards urban tolerance; however, these species have largely been ignored in urban studies. I used Wood Storks (Mycteria americana) to determine how a wetland species of conservation concern responded to human-induced landscape change in South Florida. Specifically, my study investigated 1) resource selection of storks in roadway corridors, 2) factors influencing stork prey biomass in roadside created wetlands, 3) dietary flexibility of storks in response to human-induced landscape change, and 4) the impact of urban food subsidies on natural food limitations and stork productivity. I found that storks preferred canals and roadway corridors within the urban landscape. At a finer scale, storks selected for more natural wetland vegetation even within the urban landscape cover type. These results suggest that roadway corridors even within a highly urbanized area may provide adequate foraging habitat for storks. Factors influencing stork prey biomass in roadside created wetlands varied depending on created wetland type. I found that landscape-level vegetation and the physical properties of a created wetland were more influential in permanently inundated created wetlands whereas local-scale vegetation and hydrologic conditions were most influential in ephemeral created wetlands. Storks also selected prey that were more similar to the larger-bodied fishes in created wetlands than the smaller fishes in natural wetlands. Urban nesting storks selected prey that were more characteristic of created wetlands whereas storks nesting in natural wetlands selected prey that was more characteristic of prey found in natural wetlands. These results suggested that storks may have behavioral plasticity in foraging habitat and prey selection to adapt to some degree of human-induced rapid environmental change. Additionally, storks nesting in both urban and natural wetlands had narrow diet breadths and high productivity during optimal natural wetland conditions; however, during suboptimal natural conditions, urban stork diet expanded to include anthropogenic items, leading to increased productivity. Overall, this research provides a mechanistic understanding of how a wetland species persists, and even thrives, in an urban environment.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013554
Subject Headings: Wood stork, Mycteria americana, Human influence on nature, Nature conservation--Florida, Urban environment
Format: Document (PDF)

Title: REPRODUCTIVE RESPONSES OF THREE HERON SPECIES TO VARIABLE FORAGING CONDITIONS AND NESTING ISLAND TYPE IN A MANAGED LAKE ECOSYSTEM.
Creator: Evans, Jacquelyn D., Gawlik, Dale E., Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: The relationship between water-level fluctuations and wading bird nest numbers and nesting location is well documented, yet species-specific reproductive responses of wading birds to environmental drivers and nesting habitat type is poorly understood. Here, I compared the reproductive responses of two ecologically similar species, Snowy Egret and Tricolored Heron, to foraging conditions influenced by water management and examined the effect of nestling island type on the reproductive success...
Show moreThe relationship between water-level fluctuations and wading bird nest numbers and nesting location is well documented, yet species-specific reproductive responses of wading birds to environmental drivers and nesting habitat type is poorly understood. Here, I compared the reproductive responses of two ecologically similar species, Snowy Egret and Tricolored Heron, to foraging conditions influenced by water management and examined the effect of nestling island type on the reproductive success of three wading bird species. Reproductive responses to foraging conditions were broadly similar between Snowy Egrets and Tricolored Herons, however this study revealed specific-specific differences that could lead to different population dynamics in response to management over the long-term. I also found that these two species had lower productivity at spoil islands than marsh colonies, whereas Great Egret productivity did not vary by colony type. This study demonstrates the importance of establishing species-species relationships between productivity and environmental conditions
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013550
Subject Headings: Herons--Ecology, Reproduction, Nesting behavior, Okeechobee, Lake (Fla), Species
Format: Document (PDF)

Title: TO BUILD AN INVASIVE PREDATOR: INVESTIGATING THE MECHANICAL ROLE OF LIONFISH SPINES ON DEFENSE.
Creator: Galloway, Katherine Ann, Porter, Marianne E., Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: The red lionfish, Pterois volitans, has arguably become the most successful marine invasive species to date. Yet, despite the invasion success of P. volitans, little is known about the morphology, physiology, and ecology of this species in their native and invaded habitats. The majority of recent studies have focused on the migration of P. volitans into new regions, digestion, and bacterial infections. Knowledge is lacking on the body plan of the invasive lionfish, specifically the numerous...
Show moreThe red lionfish, Pterois volitans, has arguably become the most successful marine invasive species to date. Yet, despite the invasion success of P. volitans, little is known about the morphology, physiology, and ecology of this species in their native and invaded habitats. The majority of recent studies have focused on the migration of P. volitans into new regions, digestion, and bacterial infections. Knowledge is lacking on the body plan of the invasive lionfish, specifically the numerous venomous spines that are protruding outward, making the body less streamlined. In this study we quantified the mechanical properties (bending and puncture) of the venomous spines of P. volitans, and related these properties to the cross-sectional morphology. We also documented variation in the cross-sectional morphology of spines from other lionfish species in their native regions. Lastly, we documented the dorsal spine joint morphology of the first three dorsal spines and the in situ range of motion as tissues were removed. We found that the long, numerous dorsal spines absorb more energy but are less stiff than the pelvic and anal spines. In addition, we found that the anal and pelvic spines are more effective at puncturing buccal skin from sharks and grouper. We found that the removal of connective tissue significantly changes lateral movement (abduction) for the first three dorsal spines. The removal of the fin sheath significantly alters forward movement (extension) for the first two dorsal spines. From morphology, mechanical property, and range of motion data for P. volitans, we propose that the numerous long dorsal spines are primarily used for intimidation, and are not as effective defense structures as the pelvic and anal spines. Having a substantial amount of intimidating, venomous spines may allow for the lionfish to conserve energy for other highly metabolically costly activities other than warding off predators, such as digestion and reproduction. Future studies could focus on the amount of venom in each spine, how long it takes for the venom to be made and replaced, how the venomous spines affect hydrodynamic flow, and in vivo range of motion during swimming and striking.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013512
Subject Headings: Red lionfish, Pterois volitans, Spines (Zoology), Mechanical properties of biological structures, Biomechanics
Format: Document (PDF)

Title: Efficacy of Combining 3-Bromopyruvate with Fenofibrate in Killing the Human Breast Cancer Cell Line MCF-7.
Creator: Graham, Rashean A., Hartmann, James X., Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: The goal of our research was to find a cancer treatment that was both effective and cancer specific, sparing immune and normal tissues. We evaluated the efficacy of a combinatorial treatment using the glycolytic inhibitor 3-bromopyruvate and the fatty acid metabolism inhibitor fenofibrate in cancer, immune and normal tissue cells lines. Treatment of the human breast cancer MCF-7 with 3-bromopyruvate and fenofibrate resulted in increased cell death and decreased colony formation. In the immune...
Show moreThe goal of our research was to find a cancer treatment that was both effective and cancer specific, sparing immune and normal tissues. We evaluated the efficacy of a combinatorial treatment using the glycolytic inhibitor 3-bromopyruvate and the fatty acid metabolism inhibitor fenofibrate in cancer, immune and normal tissue cells lines. Treatment of the human breast cancer MCF-7 with 3-bromopyruvate and fenofibrate resulted in increased cell death and decreased colony formation. In the immune cells known as peripheral blood mononuclear cells our combinatorial treatment displayed less toxicity than the traditional chemotherapy doxorubicin. Our combinatorial treatment displayed greater toxicity than doxorubicin towards an established breast cell line MCF- 10A, described in the literature as representing normal breast cells. We have shown for the first time a synergistic relationship between 3-bromopyruvate and fenofibrate.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013556
Subject Headings: Breast--Cancer--Treatment, bromopyruvate, Fenofibrate, MCF-7 Cells
Format: Document (PDF)

Title: THE FORM AND FUNCTION OF VERTEBRAL TRABECULAR BONE IN FULLY AQUATIC MAMMALS.
Creator: Ingle, Danielle Nicole, Porter, Marianne, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: Among vertebrates, whole-body movement is centered around the vertebral column. The bony vertebral column primarily consists of trabecular (spongy) bone that adapts in vivo to support mechanical demands respective to region, ontogeny, ecology, and locomotion. Previous work has extensively investigated the formfunction relationships of vertebral trabecular bone in terrestrial mammals, who use limb contact with a substrate as the primary support against gravity. However, we lack data from...
Show moreAmong vertebrates, whole-body movement is centered around the vertebral column. The bony vertebral column primarily consists of trabecular (spongy) bone that adapts in vivo to support mechanical demands respective to region, ontogeny, ecology, and locomotion. Previous work has extensively investigated the formfunction relationships of vertebral trabecular bone in terrestrial mammals, who use limb contact with a substrate as the primary support against gravity. However, we lack data from obligate swimming mammals whose locomotor ecology diverged from their terrestrial counterparts in two major ways: (1) body mass is supported by water’s uplifting buoyant forces and (2) swimmers power movement through dorsoventral loading of the axial body. This study examined vertebral trabecular bone mechanical properties and micoarchitecture from fully aquatic mammals, specifically sirenians (i.e. manatees) and cetaceans (i.e. dolphins and whales). We compression tested bone from several regions of the vertebral column among developmental stages in Florida manatees (Trichechus manatus latirostris) and among 10 cetacean species (Families Delphinidae and Kogiidae) with various swimming modes and diving behaviors. In addition, we microCT scanned a subset of cetacean vertebrae before subjecting them to mechanical tests. We demonstrated that in precocial manatee calves, vertebrae were the strongest and toughest in the posterior vertebral column, which may support rostrocaudal force propagation and increasing bending amplitudes towards the tail tip during undulatory swimming. Among cetaceans, we showed that greatest strength, stiffness, toughness, bone volume fraction, and degree of anisotropy were in rigidtorso shallow-divers, while properties had the smallest values in flexible-torso deep-divers. We propose that animals swimming in shallower waters actively swim more than species that conduct habitual glides during deep descents in the water column, and place comparatively greater loads on their vertebral columns. We found that cetacean bone volume fraction was the best predictor for mechanical properties. Due to the shared non-weight bearing conditions of water and microgravity, we present these data as a contribution to the body of work investigating bone adaptations in mammals that live in weightless conditions throughout life and evolutionary history.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013529
Subject Headings: Cancellous Bone, Vertebrae, Aquatic mammals, Sirenia, Cetaceans, Bones--Mechanical properties
Format: Document (PDF)

Title: EVALUATION OF THE CONTRIBUTION METAGENOMIC SHOTGUN SEQUENCING HAS IN ASSESSING POLLUTION SOURCE AND DEFINING PUBLIC HEALTH AND ENVIRONMENTAL RISKS.
Creator: Mercer, Brian, Esiobu, Nawdiuto, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: State-approved membrane filtration (MF) techniques for water quality assessments were contrasted with metagenomic shotgun sequencing (MSS) protocols to evaluate their efficacy in providing precise health-risk indices for surface waters. Using MSS, the relative numerical abundance of pathogenic bacteria, virulence and antibiotic resistance genes revealed the status and potential pollution sources in samples studied. Traditional culture methods (TCM) showed possible fecal contamination, while...
Show moreState-approved membrane filtration (MF) techniques for water quality assessments were contrasted with metagenomic shotgun sequencing (MSS) protocols to evaluate their efficacy in providing precise health-risk indices for surface waters. Using MSS, the relative numerical abundance of pathogenic bacteria, virulence and antibiotic resistance genes revealed the status and potential pollution sources in samples studied. Traditional culture methods (TCM) showed possible fecal contamination, while MSS clearly distinguished between fecal and environmental bacteria contamination sources, and pinpointed actual risks from pathogens. RNA MSS to detect all viable microorganisms and qPCR of fecal biomarkers were used to assess the possible environmental risk between runoff drainage canals and a swamp area with no anthropogenic impact. Results revealed higher levels of pathogenic bacteria, viruses, and virulence and antibiotic resistance genes in the canal samples. The data underscore the potential utility of MSS in precision risk assessment for public and biodiversity health and tracking of environmental microbiome shifts by field managers and policy makers.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013573
Subject Headings: Metagenomics, Public health--Risk assessment, Environmental risks, Water quality--Evaluation, Metagenomics--methods
Format: Document (PDF)

Title: DEVELOPMENT OF A GENETICALLY-ENCODED OXYTOCIN SENSOR TO DEFINE THE ROLE OF OXYTOCIN IN PREDICTING SOCIAL REWARD.
Creator: Mignocchi, Neymi Layne, Kwon, Hyung-Bae, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: Oxytocin (OXT), a neuropeptide synthesized in the paraventricular nucleus (PVN) of the hypothalamus, functions to increase the precedence of social stimuli and promote the development of a wide range of social behaviors. However, whether OXT has a predicting role in social reward has yet to be examined. In this study, we developed a genetically encoded, scalable OXT sensor named OXTR-iTango2 and applied this technique to define the role of OXT in learned social behaviors. OXTR-iTango2 enables...
Show moreOxytocin (OXT), a neuropeptide synthesized in the paraventricular nucleus (PVN) of the hypothalamus, functions to increase the precedence of social stimuli and promote the development of a wide range of social behaviors. However, whether OXT has a predicting role in social reward has yet to be examined. In this study, we developed a genetically encoded, scalable OXT sensor named OXTR-iTango2 and applied this technique to define the role of OXT in learned social behaviors. OXTR-iTango2 enables the combination of light- and ligand- dependent gene expression both in vitro and in vivo neural systems. In order to study the predictive role of OXT during expected socially rewarding experiences, we first conditioned animals to a social environment, and then selectively labeled OXT-sensitive ventral tegmental area dopamine (VTA-DA) neurons when animals encountered a conditioned stimulus that stood to predict a familiar social reward. Recurrent exposure to the same social stimulus normally lowered the degree of social interaction, but this reduced interaction was not observed when OXT-sensitive DA neurons were optogenetically inhibited. Thus, our findings support the notion that OXT plays a role beyond promoting social interactions, leading for a new proposed hypothesis that OXT mediation also leads to active avoidance of mundane social interactions.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013509
Subject Headings: Oxytocin, Oxytocin--Research, Social Behavior, Oxytocin--physiology, Biosensing Techniques
Format: Document (PDF)

Title: BEHAVIORAL ECOLOGY OF THE BACHMAN’S SPARROW (PEUCAEA AESTIVALIS).
Creator: Niederhauser, Joseph M., Anderson, Rindy, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: Behavioral ecology is an integrative field that span many different topics from genes and physiology to ecology and evolutionary biology. In this dissertation, I studied territoriality, behavioral plasticity and syndromes, song learning, and natal dispersal in the Bachman’s sparrow (Peucaea aestivalis), an imperiled songbird that is understudied in many aspects of its behavior. In chapter one, I provide a background of behavioral ecology, the topics covered in this dissertation, and the...
Show moreBehavioral ecology is an integrative field that span many different topics from genes and physiology to ecology and evolutionary biology. In this dissertation, I studied territoriality, behavioral plasticity and syndromes, song learning, and natal dispersal in the Bachman’s sparrow (Peucaea aestivalis), an imperiled songbird that is understudied in many aspects of its behavior. In chapter one, I provide a background of behavioral ecology, the topics covered in this dissertation, and the Bachman’s sparrow. In chapter two, I determine if the ideal despotic distribution applies to Bachman’s sparrows. In chapter three, I examine whether two common personality traits, aggressiveness and boldness, are consistent over time and form a behavioral syndrome, or if they change in relation to environmental variables. My last chapter infers song learning and natal dispersal strategies in Bachman’s sparrows from examining patterns song-type sharing over geographic distance.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013528
Subject Headings: Bachman's sparrow, Peucaea aestivalis, Behavioral ecology
Format: Document (PDF)

Title: INFLUENCES OF WATER QUALITY AND HABITAT RELATIONSHIPS ON THE BURROW DENSITY OF SPHAEROMA TEREBRANS.
Creator: Powell, Krystyna, Frazier, Evelyn, Proffitt, C. Edward, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: Sphaeroma terebrans, is an ecological engineer that can significantly modify the habitat of free-hanging aerial prop roots of Rhizophora mangle. The wood-boring isopod extensively burrows into red mangrove aerial prop roots for habitat and protection from desiccation and access to phytoplankton. However, the burrows created have major consequences on the mangrove habitat and aerial root inhabitants. It has been suggested that sessile species residing in aerial root communities can either...
Show moreSphaeroma terebrans, is an ecological engineer that can significantly modify the habitat of free-hanging aerial prop roots of Rhizophora mangle. The wood-boring isopod extensively burrows into red mangrove aerial prop roots for habitat and protection from desiccation and access to phytoplankton. However, the burrows created have major consequences on the mangrove habitat and aerial root inhabitants. It has been suggested that sessile species residing in aerial root communities can either encourage or discourage colonization by S. terebrans. Abiotic factors can affect the distribution and abundance of mangrove forest and are the same factors which determine the composition and abundance of organisms living on the roots. Surveys indicated that burrowing damage was found predominately in the first 20 cm of the root tip. Exploratory Structural Equation Modeling (SEM) was used to test multivariate hypothesized models looking at habitat relationships with S. terebrans in aerial root communities. Temperature and dissolved oxygen were shown to be important drivers in affecting submerged root length of aerial roots. Ultimately, the indirect effects between these parameters proved to be stronger in influencing the barnacle – isopod association, which causes direct negative effects on submerged root length. Colonial tunicates showed weak effects in masking aerial roots from the damaging barnacle – isopod association. Chlorophyll a was used as a proxy for phytoplankton biomass and proved to be less influential than habitat protection for S. terebrans. Results highlight the need for experimentation in addition to modeling in order to determine the mechanisms influencing aerial root community inhabitants and further effects on the habitat.
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Date Issued: 2020
PURL: http://purl.flvc.org/fau/fd/FA00013544
Subject Headings: Sphaeroma, Isopoda, Rhizophora mangle, Red mangrove--Ecology, Prop roots, Habitats
Format: Document (PDF)

Current FAU Student Theses and Dissertations 2007 to date (288)	+ -
Legacy FAU Student Theses and Dissertations 1967-2006 (54)	+ -
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Cellular signal transduction (31)	+ -
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