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Diversity and selection in the major histocompatibility complex
Title
Diversity and selection in the major histocompatibility complex: DQA and immune function in IRL and Atlantic bottlenose dolphins (Tursiops truncatus).
Creator
Ferrer, Tatiana., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description
The Major Histocompatibility Complex (MHC) encodes proteins critical to the vertebrate immune response; therefore MHC diversity is an indicator of population health. I have (1) Isolated exon 2 of the class II gene DQA in Tursiops truncatus in the North Indian River Lagoon (IRL) (n=17), South IRL (n=29) and adjacent Atlantic waters (n=20), (2) assessed genetic variability between groups, (3) developed a method to genotype individuals, (4) typed 11 unique alleles in 66 individuals, (5) detected...
Show moreThe Major Histocompatibility Complex (MHC) encodes proteins critical to the vertebrate immune response; therefore MHC diversity is an indicator of population health. I have (1) Isolated exon 2 of the class II gene DQA in Tursiops truncatus in the North Indian River Lagoon (IRL) (n=17), South IRL (n=29) and adjacent Atlantic waters (n=20), (2) assessed genetic variability between groups, (3) developed a method to genotype individuals, (4) typed 11 unique alleles in 66 individuals, (5) detected geographic patterns of diversity between estuarine and coastal individuals (FST=0.1255, p<0.05), (6) found evidence of positive selection centered in the binding pockets P1, P6 and P9 of the peptide binding region (w=2.08), (7) found that patterns of polymorphism did not closely match patterns of diversity in neutral markers, (8) performed a pilot study with Orcinus orca. The initial findings highlight the need for further comparative work and suggest that silent mutations are not neutral.
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Date Issued
2013
PURL
http://purl.flvc.org/fcla/dt/3362335
Subject Headings
Major histocompatibility complex, Immunogenetics, Molecular genetics, Endocrine disrupting chemicals, Dolphins, Geographical distribution, Population genetics, Social behavior in animals
Format
Document (PDF)