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Pages
- Title
- Studies towards a catalytic asymmetric isomerization of manganese complexed alkynes to allenes using chiral bases.
- Creator
- He, Chang., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
- Abstract/Description
-
The conversion of alkynyl carbonyls to allenyl carbonyls via manganese mediated coordination followed by a base-catalyzed isomerization was carried out using a range of chiral and achiral amine bases. Chiral amidine and chiral DBU derivatives were synthesized to carry out the isomerization enantioselectively. We employed HPLC equipped with a chiral column to determine the enantiomeric excess. We also proved that the mechanism of that the manganese-coordinated alkyne/allene rearrangement...
Show moreThe conversion of alkynyl carbonyls to allenyl carbonyls via manganese mediated coordination followed by a base-catalyzed isomerization was carried out using a range of chiral and achiral amine bases. Chiral amidine and chiral DBU derivatives were synthesized to carry out the isomerization enantioselectively. We employed HPLC equipped with a chiral column to determine the enantiomeric excess. We also proved that the mechanism of that the manganese-coordinated alkyne/allene rearrangement reaction involved an intermediate cumenolate. It was also confirmed that amine base with pKa lower than that of DBU (pKa = 13.6) would not carry out the isomerization. Alkoxy base were also used in isomerization and the mechanism was also investigated.
Show less - Date Issued
- 2009
- PURL
- http://purl.flvc.org/FAU/192983
- Subject Headings
- Enzyme kinetics, Organometallic compounds, Synthesis, Asymmetric synthesis, Methathesis (Chemistry), Carbonyl compounds, Synthesis
- Format
- Document (PDF)
- Title
- Studies Towards Synthesis of Paraconic Acids.
- Creator
- Soto, Karenia, Dragojlovic, Veljko
- Abstract/Description
-
FAU's Office of Undergraduate Research and Inquiry hosts an annual symposium where students engaged in undergraduate research may present their findings either through a poster presentation or an oral presentation.
- Date Issued
- 2011
- PURL
- http://purl.flvc.org/fau/fd/FA00005450
- Format
- Document (PDF)
- Title
- Studies towards synthesis of paraconic acids.
- Creator
- Soto, Karenia, Dragojlovic, Veljko, Harriet L. Wilkes Honors College, Florida Atlantic University
- Abstract/Description
-
Paraconic acids are naturally occurring compounds synthesized by bacteria and fungi. They are known for having antitumor and antibiotic properties. By use of a phasevanishing (PV) halolactonization with PTFE (Teflon) as a phase screen, we were able to synthesize precursors to these compounds. The PV-PTFE halolactonization of alkenoic acids with iodine monochloride gave the corresponding iodolactones, while halolactonization with bromine gave the corresponding bromolactone. Halolactonization...
Show moreParaconic acids are naturally occurring compounds synthesized by bacteria and fungi. They are known for having antitumor and antibiotic properties. By use of a phasevanishing (PV) halolactonization with PTFE (Teflon) as a phase screen, we were able to synthesize precursors to these compounds. The PV-PTFE halolactonization of alkenoic acids with iodine monochloride gave the corresponding iodolactones, while halolactonization with bromine gave the corresponding bromolactone. Halolactonization of 4-pentenoic acid with water as a solvent also gave good results. Future experiments will explore the synthesis of further precursors by means of a photolytic carbonylation of iodolactone and a phenylation-ruthenium tetroxide oxidation sequence.
Show less - Date Issued
- 2011
- PURL
- http://purl.flvc.org/fau/fd/FA00003607
- Format
- Document (PDF)
- Title
- Studies towards the synthesis of Cephalostatin 1.
- Creator
- Wu, Mao, Florida Atlantic University, Kerr, Russell G.
- Abstract/Description
-
Cephalostatin 1 has been isolated from the colonial marine worm Cephalodiscus gilchristi (Phylum Hemichordata) in the Indian Ocean. It has shown biomedical potential in preclinical trials as a cell growth inhibitor, active against U. S. murine P388 lymphocyte leukemia ED5010^-7-10^-9 μ/ml and a potent growth inhibitor against solid tumor types. Our research is focusing on developing methodology to produce disteroidal pyrazine and the introduction of delta 14, 15 unsaturation and C-16 hydroxyl...
Show moreCephalostatin 1 has been isolated from the colonial marine worm Cephalodiscus gilchristi (Phylum Hemichordata) in the Indian Ocean. It has shown biomedical potential in preclinical trials as a cell growth inhibitor, active against U. S. murine P388 lymphocyte leukemia ED5010^-7-10^-9 μ/ml and a potent growth inhibitor against solid tumor types. Our research is focusing on developing methodology to produce disteroidal pyrazine and the introduction of delta 14, 15 unsaturation and C-16 hydroxyl groups in Cephalostatin 1A. The synthetic intermediates may be useful for biosynthetic studies and also analogues can be used to study structure-activity relationships.
Show less - Date Issued
- 1995
- PURL
- http://purl.flvc.org/fcla/dt/15169
- Subject Headings
- Bioactive compounds, Pharmaceutical chemistry, Cancer--Research, Pyridazines
- Format
- Document (PDF)
- Title
- Study abroad in Grenoble, France.
- Creator
- Selby, Megan
- Date Issued
- 2005-06
- PURL
- http://purl.flvc.org/fcla/dt/11559
- Subject Headings
- Expeditionary Learning Outward Bound, Foreign study, Education--Florida, Students--Travel--Europe
- Format
- Document (PDF)
- Title
- Study abroad: London.
- Creator
- Grant, Jensen
- Date Issued
- 2005-06
- PURL
- http://purl.flvc.org/fcla/dt/11547
- Subject Headings
- Expeditionary Learning Outward Bound, Foreign study, Education--Florida, Students--Travel--Europe
- Format
- Document (PDF)
- Title
- Study and Design of a PIFA for a Human-Implanted RFID Communication System.
- Creator
- Jean-Charles, Yves-Thierry, Ungvichian, Vichate, Florida Atlantic University
- Abstract/Description
-
This effort studies the implementation of a biocompatible Planar Inverted-F Antenna (PIF A) for the Medical Instrument Communication System (MICS) frequency band of 402 to 405 MHz for RFID applications. The basic electromagnetic properties of human tissues are investigated at 403 .5 MHz. Then, the radiation characteristics of submerged wire antenna are evaluated in order to approximate the effects of multilayered media on a PIFA's performance. The PIFA is assessed using Ansoft's Finite...
Show moreThis effort studies the implementation of a biocompatible Planar Inverted-F Antenna (PIF A) for the Medical Instrument Communication System (MICS) frequency band of 402 to 405 MHz for RFID applications. The basic electromagnetic properties of human tissues are investigated at 403 .5 MHz. Then, the radiation characteristics of submerged wire antenna are evaluated in order to approximate the effects of multilayered media on a PIFA's performance. The PIFA is assessed using Ansoft's Finite Element Method based electromagnetic evaluation software, HFSS v.l 0. The electrical parameters of the antenna in relation to the surrounding environment, notably air, silicone capsule, and a three-layered bio-media, are presented. The analysis shows that the resonant frequency as well as the electrical performance of the design is significantly affected by the physical dimensions and the relative permittivity of the dielectric materials covering the antenna.
Show less - Date Issued
- 2008
- PURL
- http://purl.flvc.org/fau/fd/FA00012529
- Subject Headings
- Radio frequency identification systems--Design, Antenna arrays--Design, Medical telematics, Health services administration--Technological innovations
- Format
- Document (PDF)
- Title
- A study examining the impact of vocabulary instruction on the vocabulary growth and acquisition of adults enrolled in a community college developmental reading course.
- Creator
- Robson, Jodi McGeary., College of Education, Department of Curriculum, Culture, and Educational Inquiry
- Abstract/Description
-
The purpose of this study was to examine the effects of four instructional methods - context clues, definition, elaboration technique, or word parts and word families- on the vocabulary growth and acquisition of adults enrolled in a community college developmental reading course. The study investigated whether performance in any or all of the four instructional methods was moderated by age or language. Seventy-three respondents participated in the study. Participants were enrolled in one of...
Show moreThe purpose of this study was to examine the effects of four instructional methods - context clues, definition, elaboration technique, or word parts and word families- on the vocabulary growth and acquisition of adults enrolled in a community college developmental reading course. The study investigated whether performance in any or all of the four instructional methods was moderated by age or language. Seventy-three respondents participated in the study. Participants were enrolled in one of five sections of College Reading Preparatory II (REA0002) offered in the Spring of 2009 at Indian River State College in Fort Pierce, Florida. All five sections of REA0002 were taught by the same professor, a tenured faculty member, chair of the developmental reading department and Associate Professor of Developmental Reading at Indian River State College. The instruction and tests in all five sections of REA0002 were consistent with the research design which insured continuity and consistency in the use of the four instructional methods. All participants received the same treatment and quizzes. During the course of the study, participants first received a pretest, then the treatment or instruction, followed by an instructional quiz, and a delayed post-test was administered at the end of the study. An analysis of the data, which included the pretest, instructional quizzes with four quizzes independently and then combined for an aggregate score for an immediate post-test, and the delayed post-test, yielded mixed results. The four instructional quizzes independently showed definition instruction to have the highest positive impact on student learning. In a measure of gains from pretest to instructional quizzes immediately after treatment, significant improvement in student learning was found only with word parts instruction., In a measure of performance from pretest to immediate post-test (aggregate score of instructional quizzes) there was a significant gain in students' vocabulary competence, and from pretest to delayed post-test there was a significant decrement in students' vocabulary competence. Age and language moderated vocabulary competence. Further tests of equivalency were mixed and should be interpreted cautiously, as there were a very small number of students in the group of 25 years or older and non-native English speakers.
Show less - Date Issued
- 2009
- PURL
- http://purl.flvc.org/FAU/369389
- Subject Headings
- Language and languages, Study and teaching, Academic achievement, Language experience approach in education, Educational tests and measurements, Vocabulary, Study and teaching
- Format
- Document (PDF)
- Title
- Study group table west wing at Wimberly Library, Boca Raton campus in 2014.
- Creator
- Florida Atlantic University
- Abstract/Description
-
FAU Libraries group study table on the west wing second floor. Note a rare moment that the table behind them is empty. Wimberly library Boca Raton campus.
- Date Issued
- 2014
- PURL
- http://purl.flvc.org/fau/fd/faua0000677
- Subject Headings
- Florida Atlantic University -- History, Florida Atlantic University -- Library, Florida Atlantic University -- Photographs, Florida Atlantic University -- Archives
- Format
- Image (JPEG)
- Title
- A study in drought physiology of four xerophytic turfgrasses.
- Creator
- Wedge, David Earl, Florida Atlantic University, Austin, Daniel F.
- Abstract/Description
-
Home owners and local and regional governments in Florida desire low maintenance ground covers. Therefore four xerophytic turfgrasses were evaluated for persistence. Turfgrasses were grown since late July 1990, along an access road to a public park and Interstate 95 in West Palm Beach, Florida. Percent cover, leaf water potential, and quantitative chlorophyll levels were determined for commercial Argentine Bahiagrass (Paspalum notatum) and three experimental grasses: Bahiagrass cv. Rapid...
Show moreHome owners and local and regional governments in Florida desire low maintenance ground covers. Therefore four xerophytic turfgrasses were evaluated for persistence. Turfgrasses were grown since late July 1990, along an access road to a public park and Interstate 95 in West Palm Beach, Florida. Percent cover, leaf water potential, and quantitative chlorophyll levels were determined for commercial Argentine Bahiagrass (Paspalum notatum) and three experimental grasses: Bahiagrass cv. Rapid Cover Polycross (RCP-2) (Paspalum notatum); St. Augustinegrass cv. FX-10 (Stenotaphyrum secundatum); and Buffalograss cv. Prairie (Buchloe dactyloides) after 12 months of biweekly irrigation and natural rainfall. Argentine Bahiagrass and St. Augustinegrass cv. FX-10 were the best performers while Buffalograss cv. prairie and RCP-2 were poor performers in this study.
Show less - Date Issued
- 1992
- PURL
- http://purl.flvc.org/fcla/dt/14798
- Subject Headings
- Turfgrasses, Ground cover plants--Florida, Xeriscaping
- Format
- Document (PDF)
- Title
- STUDY OF A PHOTOACOUSTIC CELL FOR USE AS AN OPTICAL POWER METER.
- Creator
- GUTHRIE, RICHARD WILLIAM, Florida Atlantic University
- Abstract/Description
-
The thesis first discusses the history of the photoacoustic effect, concentrating on the early history and recent investigations of the effect in solids. It then develops the theoretical description of a cell with the front window replaced with a thermally-conductive opaque membrane. The dissertation then describes the experiments performed using this and other cell designs and analyzes the results to show that the data are consistent with the theory. Finally, the system performance as a...
Show moreThe thesis first discusses the history of the photoacoustic effect, concentrating on the early history and recent investigations of the effect in solids. It then develops the theoretical description of a cell with the front window replaced with a thermally-conductive opaque membrane. The dissertation then describes the experiments performed using this and other cell designs and analyzes the results to show that the data are consistent with the theory. Finally, the system performance as a photodetector is analyzed and recommendations for improvements and future investigations made. The Appendix describes the conventional (Rosencwaig-Gersho) theory.
Show less - Date Issued
- 1983
- PURL
- http://purl.flvc.org/fcla/dt/14150
- Subject Headings
- Optoacoustic spectroscopy
- Format
- Document (PDF)
- Title
- A study of ambient noise in shallow water.
- Creator
- Pirie, Rachel Louise., Florida Atlantic University, Glegg, Stewart A. L., College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
- Abstract/Description
-
This thesis describes a series of measurements that took place over the duration of one year on the South Florida Testing Facility (SFTF) range, Dania, in order to survey the shallow water ambient acoustic environment. Three groups of data sets were taken in December 1998, May 1999 and July 1999. The data was collected using the Ambient Noise Sonar (ANS) that was developed in the Ocean Engineering department at Florida Atlantic University. The ambient acoustic environment was found to be...
Show moreThis thesis describes a series of measurements that took place over the duration of one year on the South Florida Testing Facility (SFTF) range, Dania, in order to survey the shallow water ambient acoustic environment. Three groups of data sets were taken in December 1998, May 1999 and July 1999. The data was collected using the Ambient Noise Sonar (ANS) that was developed in the Ocean Engineering department at Florida Atlantic University. The ambient acoustic environment was found to be highly variable with two main components. Boat noise was found to primarily be associated with the inlet and a source of snapping shrimp situated on the shallow water 30ft reef became apparent through 24hr observation. The effects of adverse weather were not studied due to the absence of this source during the measurement periods.
Show less - Date Issued
- 1999
- PURL
- http://purl.flvc.org/fcla/dt/15738
- Subject Headings
- Underwater acoustics, Noise--Measurement, Acoustic surface waves
- Format
- Document (PDF)
- Title
- A STUDY OF APPROACHES AND PROCESSES OF RESOLVING EMPLOYEE/UNION GRIEVANCES IN BUSINESS AND INDUSTRY.
- Creator
- EVANS, JEAN C., Florida Atlantic University, Abbott, Jarold G., College for Design and Social Inquiry, School of Public Administration
- Abstract/Description
-
This descriptive study investigates and highlights the approaches and processes of resolving employee/union grievances in private business and industry in the United States. Dealing exclusively with unionized companies, the study examines the nature of grievances in the industrial world, contractual and actual approaches and processes followed in resolving grievances (union-management discussions, mediation, arbitration, right to strike, etc.), and analyzes the advantages and disadvantages of...
Show moreThis descriptive study investigates and highlights the approaches and processes of resolving employee/union grievances in private business and industry in the United States. Dealing exclusively with unionized companies, the study examines the nature of grievances in the industrial world, contractual and actual approaches and processes followed in resolving grievances (union-management discussions, mediation, arbitration, right to strike, etc.), and analyzes the advantages and disadvantages of the various approaches and processes. Resources include research of current literature and a survey of practitioners in the industrial relations field - union officials, industrial relations executives from business, and labor arbitrators.
Show less - Date Issued
- 1975
- PURL
- http://purl.flvc.org/fcla/dt/13698
- Subject Headings
- Sociology, Industrial and Labor Relations
- Format
- Document (PDF)
- Title
- STUDY OF BANDWIDTH ALLOCATION STRATEGIES FOR NETWORKS INTEGRATING VOICE AND DATA.
- Creator
- IZQUIERDO, MICHAEL ROBERT., Florida Atlantic University, Ilyas, Mohammad
- Abstract/Description
-
The purpose of this thesis is to study three types of bandwidth allocation strategies for a network integrating voice and data, commonly referred to as Integrated Services Digital Network or ISDN, using GPSS V simulati ons. The strategies are Non-Boundary, Movable-Boundary, and Non- and Movable- Boundary with Digital Speech Interpolation. The theoretical behavior of each strategy is discussed. Exact solutions for small systems with one or two slots is shown along with approximations for...
Show moreThe purpose of this thesis is to study three types of bandwidth allocation strategies for a network integrating voice and data, commonly referred to as Integrated Services Digital Network or ISDN, using GPSS V simulati ons. The strategies are Non-Boundary, Movable-Boundary, and Non- and Movable- Boundary with Digital Speech Interpolation. The theoretical behavior of each strategy is discussed. Exact solutions for small systems with one or two slots is shown along with approximations for larger systems. General descriptions of the GPSS models for each strategy is provided. The GPSS model source code for each strategy is presented in the Appendix. Simulation is used to explore the effects of the service time ratio a of voice and data on system performance. Also, the performance of Time Division Multiplex or TDM systems with 24 channels, commonly referred to as Tl, is shown.
Show less - Date Issued
- 1987
- PURL
- http://purl.flvc.org/fcla/dt/14355
- Subject Headings
- Integrated services digital networks, Time management
- Format
- Document (PDF)
- Title
- A study of Black nursing faculty: Factors that influenced completion of graduate-level degrees.
- Creator
- Eastmond-Robinson, June Patricia., Florida Atlantic University, Jurenas, Albert C.
- Abstract/Description
-
Nursing education must attract qualified minorities to faculty positions in order to provide role models for students, to increase diversity, and to fulfill the need for new faculty. African-American faculties continue to be underrepresented in higher education and specifically in nursing education. The purpose of this study was to identify factors that influenced currently employed Black nursing faculty to persist in their education until completion of the Master of Science in Nursing degree...
Show moreNursing education must attract qualified minorities to faculty positions in order to provide role models for students, to increase diversity, and to fulfill the need for new faculty. African-American faculties continue to be underrepresented in higher education and specifically in nursing education. The purpose of this study was to identify factors that influenced currently employed Black nursing faculty to persist in their education until completion of the Master of Science in Nursing degree. Current trends in national demographics demonstrate an increasingly diverse population. likewise, a changing health care delivery system that has mandated decreased hospital length of stay requires nurses to advocate and educate the client in self-care in a shorter time frame. Lack of knowledge of cultural patterns in minority clients has been shown to contribute to problems of non-compliance with medically prescribed treatments (Pacquino, 1995). Whatever becomes of the evolving debate over insurance reimbursement, nursing care of clients will continue to be an important component. Sensitivity to culture and ethnic patterns is crucial in maintaining the client's health and independence. This research describes a sample of African-American nursing faculty whose names were systematically selected from The Directory of Black Nursing Faculty, (Tucker, 1991). A questionnaire was developed by the researcher that contains thirty Likert-style and open-ended questions. The instrument was grouped into 3 categories: demographics, educational influences, and psychosocial influences. One hundred thirty-nine questionnaires were mailed to the sample. After 2 mailings, the response rate was 56%. The findings revealed that program completion for this group was influenced by, (a) financial assistance. Over eighty percent reported this was essential, (b) a supportive school of nursing environment that accounted for the second highest degree of importance, (c) a diverse support system including student networking, faculty mentoring/advising, family, and friends, and (d) the imperative of high self-esteem needed to sustain the African-American student nurse in a perceived hostile educational environment.
Show less - Date Issued
- 1999
- PURL
- http://purl.flvc.org/fcla/dt/12620
- Subject Headings
- Nursing schools--Faculty, African American nurses, Nursing--Study and teaching (Graduate)
- Format
- Document (PDF)
- Title
- A STUDY OF BLACK-WHITE MALE INCOME DIFFERENTIALS - 1950, 1960, 1970.
- Creator
- BOLES, KEITH EDWIN., Florida Atlantic University, Hemley, David D., College of Business, Department of Economics
- Abstract/Description
-
Alternative models to explain the variability in income differentials. between Black males and White males over thirty-two Standard Metropolitan Statistical Areas (SMSAs) were estimated by ordinary least squares using cross-sectional data for each of three points in time - 1950, 1960, and 1970. Two models were tested for each time period . The Becker-type model used a Black-White male median income ratio as a dependent variable with age, education, three occupational mix variables, and...
Show moreAlternative models to explain the variability in income differentials. between Black males and White males over thirty-two Standard Metropolitan Statistical Areas (SMSAs) were estimated by ordinary least squares using cross-sectional data for each of three points in time - 1950, 1960, and 1970. Two models were tested for each time period . The Becker-type model used a Black-White male median income ratio as a dependent variable with age, education, three occupational mix variables, and current population as the independent variables. The second model used the same variables with the exception that current population was replaced by population lagged ten years. All variables were in Black-White ratio form. The results are of interest to the student of the economics of discrimination, since the methodology can be applied to the examination and comparison between any two categories of people.
Show less - Date Issued
- 1973
- PURL
- http://purl.flvc.org/fcla/dt/13562
- Subject Headings
- Income distribution--United States--Statistics, Income distribution--United States--Mathematical models, African Americans--Economic conditions
- Format
- Document (PDF)
- Title
- Study of Cell Penetrating Peptide Uptake and Cancer Cell Discrimination with Raman Spectroscopy and Microscopy.
- Creator
- Cosme, Patrick Jason, Terentis, Andrew C., Florida Atlantic University, Charles E Schmidt College of Science, Department of Chemistry and Biochemistry
- Abstract/Description
-
Cell penetrating peptides (CPPs) are short sequences of amino acids that excel in crossing the cellular membrane without inducing cytotoxicity Interest in these peptides stem from their ability to be attached, and grant their penetrating properties to, a variety of cargo In this work we have combined the application of Confocal Raman Microscopy (CRM) and Atomic Force Microscopy for the first time to examine the interactions of unlabeled Transportan (TP), one of the most well studied CPPs,...
Show moreCell penetrating peptides (CPPs) are short sequences of amino acids that excel in crossing the cellular membrane without inducing cytotoxicity Interest in these peptides stem from their ability to be attached, and grant their penetrating properties to, a variety of cargo In this work we have combined the application of Confocal Raman Microscopy (CRM) and Atomic Force Microscopy for the first time to examine the interactions of unlabeled Transportan (TP), one of the most well studied CPPs, with mammalian cells CRM’s capability to discriminate control and treated cell groups was verified by principal component analysis (PCA) and linear discriminant analysis (LDA) and was 93-100% accurate We’ve determined that at a concentration of 20 μM TP enters cells through a non-endocytotic mechanism, has a high affinity for the cytoplasm and membranes, and results in a significant increase in cellular stiffness Our work provides the first direct evidence of this cell-stiffening phenomenon SFTI-1, the smallest member of a bicyclic, cysteine rich class of CPPs, was examined by CRM to determine the potential role of cyclic structure on cellular uptake The peptide, along with monocyclic and linear analogs was heavy isotope labeled and incubated with mammalian cells at numerous concentrations and timespans Our work is the first SFTI-1 uptake study forgoing the use of fluorophore conjugates, which have been linked to artificial cellular uptake We demonstrate herein the absence of any CRM detectable uptake, providing the first evidence that SFTI-1 may not be a CPP Finally, CRM was applied to the discrimination of normal and basal cell carcinoma cells obtained from the same donor The use of patient matched cells avoids the normal biochemical variations that exist among individuals, ensuring that discrimination is based solely on the cell’s diseased state CRM spectra, analyzed by PCA and LDA, were capable of spectral discrimination with 100% accuracy Major differences in the cancerous cells were an increase in lipids and nucleic acids, and an overall decrease in protein We also demonstrate an enhancement in Raman signal through the use of an aluminum foil substrate, providing a practical approach for measuring cells with thin morphologies
Show less - Date Issued
- 2016
- PURL
- http://purl.flvc.org/fau/fd/FA00004756
- Subject Headings
- Peptides--Analysis, Peptides--Therapeutic use, Peptides--Physiological transport, Cellular signal transduction, Raman spectroscopy, Infrared spectroscopy
- Format
- Document (PDF)
- Title
- Study of cell penetrating peptides with Raman spectroscopy and microscopy.
- Creator
- Ye, Jing., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
- Abstract/Description
-
Cell penetrating peptides (CPPs) have drawn the attention of researchers due to their ability to internalize large cargos into cells including cancer cells. The mechanism(s) with which the peptides enter the cell, however, is/are not clear and full of controversy. The peptide conformations and their microenvironment in live cells had been unknown until the development of a technique developed in our lab. As a first demonstration of principle, penetratin, a 16-residue CPP derived from the...
Show moreCell penetrating peptides (CPPs) have drawn the attention of researchers due to their ability to internalize large cargos into cells including cancer cells. The mechanism(s) with which the peptides enter the cell, however, is/are not clear and full of controversy. The peptide conformations and their microenvironment in live cells had been unknown until the development of a technique developed in our lab. As a first demonstration of principle, penetratin, a 16-residue CPP derived from the Antennapedia homeodomain protein of Drosophila, was measured in single, living melanoma cells. Carbon-13 labeling of the Phe residue of penetratin was used to shift the intense aromatic ring-breathing vibrational mode from 1003 to 967 cm-1, thereby enabling the peptide to be traced in cells. Difference spectroscopy and principal components analysis (PCA) were used independently to resolve the Raman spectrum of the peptide from the background cellular Raman signals., On the basis of the position of the amide I vibrational band in the Raman spectra, the secondary structure of the peptide was found to be mainly random coil and b-strand in the cytoplasm, and possibly assembling as b-sheets in the nucleus. Next, label-free transportan was studied with the same methodology. The peptide, besides predominantly a-helix, adopted a significant portion of b-sheet conformation in the cytoplasm and nucleolus, which is different from the peptide in aqueous solution. The peptide microenvironment was also probed through H-bonding reported by the tyrosine Fermi doublet. Transportan displayed a tendency to accumulate in the cytoplasm over time which was unlike penetratin, which concentrated in the nucleus. The relative concentration of CPPs in various locations of live melanoma cells was directly estimated from the Raman spectra using average Phe concentration in the cell as an internal standard., The rapid entry and almost uniform cellular distribution of both peptides, as well as the lack of correlation between peptide and lipid Raman signatures, indicated that the mechanism of CPP internalization under the conditions of study was probably non-endocytotic. Last, transportan and penetratin were studied using polarized Raman spectroscopy for more detailed vibrational spectroscopic information of the two peptides in water and TFE solutions. The majority of the bands in the Raman spectra of the peptides were highly polarized, consistent with the high symmetry of aromatic ring side chain vibrational bands dispersed throughout the spectra. This work has provided new insights into the structure of CPPs in live cells and in solutions.
Show less - Date Issued
- 2011
- PURL
- http://purl.flvc.org/FAU/3342344
- Subject Headings
- Peptides, Analysis, Infrared spectroscopy, Raman spectroscopy, Cellular signal transduction
- Format
- Document (PDF)
- Title
- A STUDY OF COMMUNITY EDUCATION PARTICIPATION AND SELECTED VARIABLES AND THEIR RELATIONSHIP TO AN INDIVIDUAL'S ATTITUDES TOWARD THE PUBLIC SCHOOLS.
- Creator
- GEHRET, EDWARD F., Florida Atlantic University, Kerensky, Vasil M.
- Abstract/Description
-
The purpose of the study was to determine the role participation and other selected variables (i.e., age, sex, race, education, etc.) play in an individual's attitude toward the public schools. The research was conducted in the community of Dade County, Florida, utilizing forty-one of the fifty-eight school centers designated as Community Schools. Hypotheses. (1) There is no significant difference in the mean attitude scores based upon participation groups at the .05 level of significance. (2...
Show moreThe purpose of the study was to determine the role participation and other selected variables (i.e., age, sex, race, education, etc.) play in an individual's attitude toward the public schools. The research was conducted in the community of Dade County, Florida, utilizing forty-one of the fifty-eight school centers designated as Community Schools. Hypotheses. (1) There is no significant difference in the mean attitude scores based upon participation groups at the .05 level of significance. (2) There is no significant difference in the mean attitude scores of Community School groups by years of Community Education implementation at the .05 level of significance. (3) There is no linear relationship between attitude scores and the stated demographic variables at the .05 level of significance. (4) There is no significant differences in the perceived influence of participation between the defined participation groups at the .05 level of significance. Results of the Study. Hypothesis Number 1 - the null hypothesis was rejected. Hypothesis Number 2 - the null hypothesis was not rejected. Hypothesis Number 3 - the null hypothesis was rejected. Hypothesis Number 4 - the null hypothesis was not rejected. The significant demographic variables are: race, children in school, marital status, and occupation. An R('2) test of significance discloses that 12.25 percent of the variability in the dependent variable can be accounted for by the demographic and participation variables. Conclusions. (1) Residents within Dade County, who participated in the Community Education registration of September 1981, generally have positive attitudes toward the public schools. (2) The more positive attitudes were found among respondents who had had some sort of K-12 related participation experiences. (3) School related participation, whether it was Community School or K-12 types of experiences, produces positive attitudes toward the public schools. (4) Private school parents have significantly lower attitudes toward the public schools. (5) Skilled laborers have significantly more positive attitudes toward the public schools. Implications. (1) The research results and the review of the literature suggest the need for additional attitudinal change strategies once participation has begun. (2) The process of Community Education holds the promise of attitudinal change. Programs alone hold very little hope for positive attitude gains.
Show less - Date Issued
- 1982
- PURL
- http://purl.flvc.org/fcla/dt/11813
- Subject Headings
- Community and school--Florida--Miami-Dade County, Community schools--Florida--Miami-Dade County
- Format
- Document (PDF)
- Title
- A study of community-based organizations and their ability to remedy political alienation and social isolation.
- Creator
- Brown, Tara-Lynn., Florida Atlantic University, Turner, Robyne, Dorothy F. Schmidt College of Arts and Letters, Department of Political Science
- Abstract/Description
-
Many inner-city neighborhoods face a vast array of social and economic problems. These problems are partly due to crime and the lack of employment, housing & communication with local government. Likewise, participation in the political system is low within these neighborhoods as well. As a result, feelings of political alienation & social isolation may be found. This body of work tests for the presence of political alienation & social isolation within community-based organizations (CBOs) of...
Show moreMany inner-city neighborhoods face a vast array of social and economic problems. These problems are partly due to crime and the lack of employment, housing & communication with local government. Likewise, participation in the political system is low within these neighborhoods as well. As a result, feelings of political alienation & social isolation may be found. This body of work tests for the presence of political alienation & social isolation within community-based organizations (CBOs) of West Palm Beach, FL. By looking at the presence and formation of CBOs, this work will measure if CBOs can remedy feelings of alienation & isolation, increase political participation & impact government policy-making.
Show less - Date Issued
- 1995
- PURL
- http://purl.flvc.org/fcla/dt/15205
- Subject Headings
- Inner cities--Florida--West Palm Beach, Community organization, African Americans--Social conditions, Political participation--Florida--West Palm Beach, Alienation (Social psychology)
- Format
- Document (PDF)
