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Pages
- Title
- Cell cycle analysis of primary sponge cell cultures.
- Creator
- Schippers, K. J., Martens, D. E.,, Pomponi, Shirley A., Wijffels, R. H.
- Date Issued
- 2011
- PURL
- http://purl.flvc.org/FCLA/DT/3318911
- Subject Headings
- Sponges--Cytology, Cell cycle, Cell proliferation, Flow cytometry
- Format
- Document (PDF)
- Title
- Cellular Basis of Sponge-Sponge Associations.
- Creator
- Conkling, Megan, Pomponi, Shirley A., Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Marine sponges interact and coexist with many different organisms. A two-sponge association between Amphimedon erina and Geodia gibberosa commonly occurs in the Florida Keys. Previous studies have only focused on the ecological influence of the association; they did not examine the cellular basis of the association. This association between A. erina and G. gibberosa was used in the development of an in vitro model to further the understanding of the cellular basis of natural sponge-sponge...
Show moreMarine sponges interact and coexist with many different organisms. A two-sponge association between Amphimedon erina and Geodia gibberosa commonly occurs in the Florida Keys. Previous studies have only focused on the ecological influence of the association; they did not examine the cellular basis of the association. This association between A. erina and G. gibberosa was used in the development of an in vitro model to further the understanding of the cellular basis of natural sponge-sponge associations. In this study, sponge cells were cultured individually and in co-cultures and their responses related to apoptosis, cell death, and proliferation were monitored using high content imaging. Co-cultured cells of species that form sponge-sponge associations did not have the same cellular responses compared to co-cultured cells of species that do not form sponge-sponge associations. Protein expression analyses demonstrated that the model that was established does not mimic the cellular response of the association in nature, but this model can be used to test in vitro cellular interactions of sponge species that do not form associations in nature. In addition, the protein expression data that were obtained revealed that sponges use similar apoptotic pathways as humans and suggest that sponge cells may shut down cell cycling in order to repair damaged DNA. This research is a small piece to the puzzle that is sponge cell culture research.
Show less - Date Issued
- 2017
- PURL
- http://purl.flvc.org/fau/fd/FA00004848, http://purl.flvc.org/fau/fd/FA00004848
- Subject Headings
- Sponges--Habitat--Florida., Marine invertebrates--Florida., Aquatic invertebrates--Cultures and culture media., Apoptosis., Cell culture., Symbiosis.
- Format
- Document (PDF)
- Title
- Characterization and optimization of in vitro assay conditions for (1,3)β-glucan synthase activity from Aspergillus fumigatus and Candida albicans for enzyme inhibition screening.
- Creator
- Wood, R. L., Miller, T. K., Wright, Amy E., McCarthy, Peter J., Taft, C. S., Pomponi, Shirley A., Selitrennikoff, Claude, Harbor Branch Oceanographic Institute
- Date Issued
- 1998
- PURL
- http://purl.flvc.org/FCLA/DT/3351974
- Subject Headings
- Aspergillus fumigatus, Candida albicans, Glucosyltransferases, In vitro, Glucan synthase, Enzyme inhibitors
- Format
- Document (PDF)
- Title
- Characterization of an interleukin 6 cytokinefamily antagonist protein from a marine sponge, Callyspongia sp.
- Creator
- Peppard, J. V., Loo, P., Sills, Matthew A., Munster, D., Pomponi, Shirley A., Wright, Amy E.
- Date Issued
- 1996
- PURL
- http://purl.flvc.org/fau/fd/FA00007266
- Subject Headings
- Sponges, Interleukin-6, Cytokines--antagonists & inhibitors
- Format
- Document (PDF)
- Title
- Characterization of genetic markers for in vitro cell line identification of the marine sponge, Axinella corrugata.
- Creator
- Lopez, Jose V., Peterson, C. L., Willoughby, Robin, Wright, Amy E., Enright, E., Zoladz, S., Pomponi, Shirley A.
- Date Issued
- 2002
- PURL
- http://purl.flvc.org/FCLA/DT/3355156
- Subject Headings
- Sponges--Research, Marine invertebrates, Genetic markers
- Format
- Document (PDF)
- Title
- Characterization of Mesophotic Coral/Sponge Habitats and Fish Assemblages in the Regions of Pulley Ridge and Tortugas from ROV Dives during R/V Walton Smith Cruises of 2012 to 2015.
- Creator
- Reed, John K., Farrington, Stephanie, David, Andrew, Harter, Stacey, Moe, Heather, Horn, Lance, Taylor, Glenn, White, Jason, Voss, Joshua, Hanisak, M. Dennis, Diaz, Maria Cristina, Pomponi, Shirley A.
- Abstract/Description
-
This report summarizes the remotely operated vehicle (ROV) surveys during four cruises from 2012 to 2015 which characterizes the mesophotic coral reef ecosystems at Pulley Ridge and Tortugas. This research is part of a grant funded by the NOAA National Centers for Coastal Ocean Science award NA11NOS4780045: “Connectivity of the Pulley Ridge - South Florida Coral Reef Ecosystem”. The University of Miami ship R/V Walton Smith was used along with the University of North Carolina at Wilmington ...
Show moreThis report summarizes the remotely operated vehicle (ROV) surveys during four cruises from 2012 to 2015 which characterizes the mesophotic coral reef ecosystems at Pulley Ridge and Tortugas. This research is part of a grant funded by the NOAA National Centers for Coastal Ocean Science award NA11NOS4780045: “Connectivity of the Pulley Ridge - South Florida Coral Reef Ecosystem”. The University of Miami ship R/V Walton Smith was used along with the University of North Carolina at Wilmington (UNCW) Super Phantom ROV and the National Marine Sanctuary Foundation (NMSF) Mohawk ROV. The cruises were a collaboration of the University of Miami, HBOI-CIOERT, NOAA Fisheries, and the UNCW-CIOERT Undersea Vehicles Program.
Show less - Date Issued
- 2017-04-26
- PURL
- http://purl.flvc.org/fau/fd/FAUIR000227
- Format
- Citation
- Title
- Common and scientific names of aquatic invertebrates from the United States and Canada: mollusks. 2nd ed.
- Creator
- Pomponi, Shirley A.
- Date Issued
- 1998
- PURL
- http://purl.flvc.org/fau/fd/FA00007454
- Subject Headings
- Aquatic invertebrates, Mollusks--United States--Nomenclature, Mollusks -- Canada -- Nomenclature
- Format
- Document (PDF)
- Title
- Conservation and Phylogeny of a Novel Family of Non‐Hox Genes of the Antp Class in Demospongiae (Porifera).
- Creator
- Richelle-Maurer, Evelyn, Boury-Esnault, Nicole, Itskovich, Valeria B., Manuel, Michael, Pomponi, Shirley A., Van de Vyver, Gisele, Borchiellini, Carole
- Date Issued
- 2006
- PURL
- http://purl.flvc.org/FCLA/DT/2796095
- Subject Headings
- Demospongiae, Homeobox genes, Marine ecosystem management, Phylogeny, Sponges --Bahamas, Sponges --Florida
- Format
- Document (PDF)
- Title
- Cruise Report. Cuba's Twilight Zone Reefs: Remotely Operated Vehicle Surveys of Deep/Mesophotic Coral Reefs and Associated Fish Communities of Cuba.
- Creator
- Reed, John K., Farrington, Stephanie, Gonzalez-Diaz, Patricia, Lopez, Linnet Busutil, Martínez-Daranas, Beatriz, Cobián Rojas, Dorka, Voss, Joshua, Hanisak, M. Dennis, Diaz, Maria Cristina, Jiang, Mingshun, Studivan, Michael, David, Andrew, Drummond, Felicia, Mendez, Juliett Gonzalez, Garcia Rodriguez, Alain, González-Sanchez, Patricia M., Fernández, Jorge Viamontes, Pérez, Daniel Estrada, Horn, Lance, White, Jason, Pomponi, Shirley A.
- Abstract/Description
-
The purpose of this research cruise was to discover and characterize, for the first time, the extent of mesophotic reefs along the entire coastline of Cuba, and to compare the health and connectivity (physical, genetic and ecological) among both mesophotic and previously well-characterized shallow reef systems in Cuba, the southeastern United States, and Gulf of Mexico (in particular, the FGBNMS and FKNMS). Moreover, the project will directly address a recommendation in the Report from...
Show moreThe purpose of this research cruise was to discover and characterize, for the first time, the extent of mesophotic reefs along the entire coastline of Cuba, and to compare the health and connectivity (physical, genetic and ecological) among both mesophotic and previously well-characterized shallow reef systems in Cuba, the southeastern United States, and Gulf of Mexico (in particular, the FGBNMS and FKNMS). Moreover, the project will directly address a recommendation in the Report from MarCuba (2015) in which the U.S. and Cuban participants in this project stated their intention to initiate an international collaboration.
Show less - Date Issued
- 2017-10-31
- PURL
- http://purl.flvc.org/fau/fd/FAUIR000226
- Format
- Citation
- Title
- Cuba's Mesophotic Coral Reefs and Associated Fish Communities.
- Creator
- Reed, John K., Gonzalez-Diaz, Patricia, Busutil, Linnet, Farrington, Stephanie, Martínez-Daranas, Beatriz, Cobián Rojas, Dorka, Voss, Joshua, Diaz, Maria Cristina, David, Andrew, Hanisak, M. Dennis, Mendez, Juliett Gonzalez, Garcia Rodriguez, Alain, González-Sanchez, Patricia M., Viamontes Fernandez, Jorge, Estrada Perez, Daniel, Studivan, Michael, Drummond, Felicia, Jiang, Mingshun, Pomponi, Shirley A.
- Date Issued
- 2018
- PURL
- http://purl.flvc.org/fau/fd/FAUIR000351
- Format
- Citation
- Title
- CUBA’S MESOPHOTIC CORAL REEFS Sponge Photo Identification Guide 1st Edition.
- Creator
- Diaz, Maria Cristina, Pomponi, Shirley A., Reed, John K., Busutil, Linnet, Garcia-Hernandez, Maria Rosa
- Abstract/Description
-
Mesophotic coral reef ecosystems (MCEs) are light-dependent benthic communities that occur deeper than shallow reefs and typically range from depths of 30 m to the bottom of the photic zone, which may extend to >150 m in some regions. MCEs represent in part an extension of shallow-water coral reef ecosystems and support a diverse assemblages of habitat-building taxa, including corals, sponges and algae, and associated fish (Reed et al., 2018). Many MCEs worldwide appear to be thriving...
Show moreMesophotic coral reef ecosystems (MCEs) are light-dependent benthic communities that occur deeper than shallow reefs and typically range from depths of 30 m to the bottom of the photic zone, which may extend to >150 m in some regions. MCEs represent in part an extension of shallow-water coral reef ecosystems and support a diverse assemblages of habitat-building taxa, including corals, sponges and algae, and associated fish (Reed et al., 2018). Many MCEs worldwide appear to be thriving compared to shallow reefs. The deep reef refugia hypothesis suggests that MCEs may be less impacted from natural and anthropogenic impacts than shallow coral reefs, and may be more stable and resilient than shallow reefs. MCEs may also act as refugia for shallow reef species through the export of fish and coral larvae. However, it is now apparent that MCEs are also vulnerable to disturbances from all facets of perturbations including climate change, bottom trawling, invasive species, and pollution. Whereas considerable data have been reported regarding the distribution, ecology, and health of Cuba’s shallow reefs, relatively little is known about the distribution, community structure and health of Cuba’s deep mesophotic reefs. Cuba’s strong marine policies and legislation has already resulted in 105 MPAs, covering nearly 25% of its insular shelf, yet overfishing, poaching, pollution and global warming are threats to these vulnerable ecosystems, as reefs worldwide are threatened.
Show less - Date Issued
- 2019
- PURL
- http://purl.flvc.org/fau/fd/FAUIR000405
- Format
- Document (PDF)
- Title
- Cytotoxic peptides from marine sponges.
- Creator
- Gulavita, N. K., Wright, Amy E., McCarthy, Peter J., Pomponi, Shirley A., Longley, Ross E.
- Date Issued
- 1996
- PURL
- http://purl.flvc.org/fau/fd/FA00007341
- Subject Headings
- Sponges, Peptides, Cytotoxins, Stereochemistry
- Format
- Document (PDF)
- Title
- Deep-water sinkholes and bioherms of south Florida and the Pourtalés Terrace-habitat and fauna.
- Creator
- Reed, John K., Pomponi, Shirley A., Weaver, Doug C., Paull, Charles K., Wright, Amy E.
- Date Issued
- 2005
- PURL
- http://purl.flvc.org/FCLA/DT/3172087
- Subject Headings
- Sinkholes --Florida, Bioherms, Benthic animals, Groundfishes, Manned undersea research stations
- Format
- Document (PDF)
- Title
- Development of sponge cell cultures for biomedical application.
- Creator
- Pomponi, Shirley A., Willoughby, Robin
- Date Issued
- 2000
- PURL
- http://purl.flvc.org/FCLA/DT/3172822
- Subject Headings
- Natural products, Pharmacognosy, Bioactive compounds, Sponges, Sponges --Cytology
- Format
- Document (PDF)
- Title
- Development of techniques for in vitro production of bioactive natural products from marine sponges.
- Creator
- Pomponi, Shirley A., Willoughby, Robin, Kaighn, M. E., Wright, Amy E.
- Date Issued
- 1997
- PURL
- http://purl.flvc.org/fau/fd/FA00007344
- Subject Headings
- Sponges, Bioactive compounds, Marine natural products, Cell culture, Growth factors, Phytohemagglutinins, Cryopreservation
- Format
- Document (PDF)
- Title
- Discobahamins A and B, new peptides from the Bahamian deep water marine sponge Discodermia sp.
- Creator
- Gunasekera, Sarath P., Pomponi, Shirley A., McCarthy, Peter J.
- Date Issued
- 1994
- PURL
- http://purl.flvc.org/FCLA/DT/3333010
- Subject Headings
- Sponges, Peptides, Marine natural products, Chemical structure
- Format
- Document (PDF)
- Title
- Discorhabdin P, a new enzyme inhibitor from a deep-water Caribbean sponge of the genus Batzella.
- Creator
- Gunasekera, Sarath P., McCarthy, Peter J., Longley, Ross E., Pomponi, Shirley A., Wright, Amy E., Lobkovsky, E., Clardy, J.
- Date Issued
- 1999
- PURL
- http://purl.flvc.org/FCLA/DT/3319100
- Subject Headings
- Sponges --Research, Marine natural products, discorhabdin P, Enzyme inhibitors
- Format
- Document (PDF)
- Title
- Discorhabdins S, T, and U, New Cytotoxic Pyrroloiminoquinones from a Deep‐Water Caribbean Sponge of the Genus Batzella.
- Creator
- Gunasekera, Sarath P., Zuleta, Ignacio A., Longley, Ross E, Wright, Amy E., Pomponi, Shirley A.
- Date Issued
- 2003
- PURL
- http://purl.flvc.org/FCLA/DT/3164103
- Subject Headings
- Sponges, Nuclear magnetic resonance spectroscopy, Antineoplastic agents, Mass spectrometry, Pharmacognosy
- Format
- Document (PDF)
- Title
- Discovery of Loxosomella vivipara (Entoprocta: Loxosomatidae) in the Marine Sponge Hippospongia cf. gossypina (Porifera: Spongiidae) in the Florida Keys.
- Creator
- Plunkett, Rachel, Pomponi, Shirley A., Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Populations of a marine invertebrate symbiont were found on the outer surface and internal spaces of a keratose sponge from a shallow bay in the Florida Keys in May 2014. A total of 24 specimens of the seagrass and reef-dwelling sponge were collected between May 2014 and August 2015 to provide material to identify both host and symbiont, and elucidate information on the nature of the association. Based on a morphological analysis via light microscopy, histology, and scanning electron...
Show morePopulations of a marine invertebrate symbiont were found on the outer surface and internal spaces of a keratose sponge from a shallow bay in the Florida Keys in May 2014. A total of 24 specimens of the seagrass and reef-dwelling sponge were collected between May 2014 and August 2015 to provide material to identify both host and symbiont, and elucidate information on the nature of the association. Based on a morphological analysis via light microscopy, histology, and scanning electron microscopy (SEM), and 99% similarity in aligned partial sequences from 28S and 18S nuclear ribosomal genes (rDNA), the symbiont was identified as the solitary entoproct Loxosomella vivipara Nielsen, 1966 (Entoprocta: Loxosomatidae). A partial sequence from the Internal Transcribed Spacer Region 2 (ITS2) of L. vivipara was registered to GenBank for the first time. The identity of the host sponge, based on a morphological investigation, is resolved as “velvet sponge” Hippospongia cf. gossypina Duchassing and Michelotti, 1864 (Demospongiae: Spongiidae). This is the first report of an entoproct commensal from Hippospongia cf. gossypina, a sponge that formerly had great commercial value when it was abundant throughout the Bahamas, Florida Keys and Gulf of Mexico. Other common sponge species at the study site were collected to investigate the host specificity of L. vivipara. Evidence that L. vivipara favorably selects the sponges Hippospongia cf. gossypina and Chondrilla nucula over other potential host sponges at the study site is provided. Commensalism is the most plausible justification for this relationship: L. vivipara is dependent on sponges for protection and food particles, while the sponges are unaffected by its presence. Further evidence of host-specific inquilinism is provided for L. vivipara associated with Hippospongia cf. gossypina, but not for L. vivipara associated with C. nucula. An inquilinistic association between an entoproct and sponge is a rare discovery only mentioned in one previous study. Sponge aquiferous qualities such as aperture and canal size, canal and choanocyte chamber arrangement, seawater pumping rate, and food particle size selection are likely factors that inhibit or enable inquilinism in sponge-entoproct associations – a potential topic for future researc
Show less - Date Issued
- 2016
- PURL
- http://purl.flvc.org/fau/fd/FA00004791, http://purl.flvc.org/fau/fd/FA00004791
- Subject Headings
- Marine invertebrates--Florida., Sponges--Habitat--Florida., Marine resources--Florida--Management., Aquatic biology., Entoprocta--Classification.
- Format
- Document (PDF)
- Title
- Diterpene metabolites from two chemotypes of the marine sponge Myrmekioderma styx.
- Creator
- Sennett, Susan H., Pomponi, Shirley A., Wright, Amy E.
- Date Issued
- 1992
- PURL
- http://purl.flvc.org/FCLA/DT/3333004
- Subject Headings
- Sponges, Diterpenes, Marine metabolites, Chemical structure
- Format
- Document (PDF)