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- Title
- CREATINE PHOSPHOKINASE ISOENZYME ANALYSIS BY SELECTIVE ACTIVATION WITH THIOLS AND INHIBITION BY ANTIBODIES. AN EVALUATION AND ADAPTATION TO AUTOMATED ANALYSIS.
- Creator
- BERNARD, DONALD JAMES., Florida Atlantic University, Schultz, Franklin A., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
- Abstract/Description
-
Analysis of creatine phosphokinase (CPK) isoenzymes is the best clinical test for diagnosis of heart disease. Two new methods for quantitative analysis of these enzymes are selective activation with thiols and specific inhibition by antibodies. These new methods and the conventionally used technique of electrophoresis are comparatively evaluated using assayed controls and cardiac patient serum samples. Results indicate that thiol activation and antibody inhibition are preferred methods for...
Show moreAnalysis of creatine phosphokinase (CPK) isoenzymes is the best clinical test for diagnosis of heart disease. Two new methods for quantitative analysis of these enzymes are selective activation with thiols and specific inhibition by antibodies. These new methods and the conventionally used technique of electrophoresis are comparatively evaluated using assayed controls and cardiac patient serum samples. Results indicate that thiol activation and antibody inhibition are preferred methods for CPK cardiac isoenzyme analysis because they have lower levels of detection, fewer false negative results, and are considerably more efficient than electrophoresis. The new techniques also are adapted to automated spectrophotometric instrumentation, which further contributes to their accuracy and procedural efficiency. Thus, thiol activation and antibody inhibition methods should provide more sensitive and reliable CPK isoenzyme analysis for critical supportive evidence in heart disease diagnosis and treatment
Show less - Date Issued
- 1979
- PURL
- http://purl.flvc.org/fcla/dt/13982
- Subject Headings
- Chemistry, Analytic
- Format
- Document (PDF)
- Title
- ELECTROCATALYTIC REACTIONS OF OXO ANIONS WITH MOLYBDENUM - CATECHOL COMPLEXES.
- Creator
- LAHR, SUZANNE KAY, Florida Atlantic University, Schultz, Franklin A., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
- Abstract/Description
-
Catalytic reductions of NO2, ClO3, and BrO3 by aqueous molybdenum-catechol complexes at pH 9 are investigated by electrochemical techniques. Binding of substrate to the molybdenum center occurs through the unshared electron pair of the substrate central atom. The molybdenum (V) and - (IV) oxidation states are catalytically active, while Mo(III) is not. A single mechanism for the reaction of Mo(V) with all three substrates is proposed, and a rate expression for this reaction is derived....
Show moreCatalytic reductions of NO2, ClO3, and BrO3 by aqueous molybdenum-catechol complexes at pH 9 are investigated by electrochemical techniques. Binding of substrate to the molybdenum center occurs through the unshared electron pair of the substrate central atom. The molybdenum (V) and - (IV) oxidation states are catalytically active, while Mo(III) is not. A single mechanism for the reaction of Mo(V) with all three substrates is proposed, and a rate expression for this reaction is derived. General features of the Mo (IV) reaction are describec. Nitrous oxide was identified by gas chromatography as a product of the Mo(V) - NO2 reaction.
Show less - Date Issued
- 1982
- PURL
- http://purl.flvc.org/fcla/dt/14118
- Subject Headings
- Molybdenum--Analysis, Catechol--Analysis
- Format
- Document (PDF)
- Title
- ELECTROCATALYTIC REDUCTION OF ACETYLENE BY MOLYBDENUM-CYSTEINE AND MOLYBDENUM-EDTA COMPLEXES.
- Creator
- LEAZENBEE, LOUIS O., Florida Atlantic University, Schultz, Franklin A., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
- Abstract/Description
-
Binuclear oxo- and sulfide-bridged Mo(V)-cysteine and Mo(V)-EDTA complexes are chosen as model compounds for nitrogenase enzyme. Both groups of complexes undergo electrochemical reduction in a single four- electron step to Mo(III) dimers. The reduced binuclear Mo (III) units dissociate into catalytically active species capable of reducing the nitrogenase substrate, acetylene. Ease of dissociation increases as oxygen is replaced by sulfur in the bridging unit. Electrocatalytic reduction...
Show moreBinuclear oxo- and sulfide-bridged Mo(V)-cysteine and Mo(V)-EDTA complexes are chosen as model compounds for nitrogenase enzyme. Both groups of complexes undergo electrochemical reduction in a single four- electron step to Mo(III) dimers. The reduced binuclear Mo (III) units dissociate into catalytically active species capable of reducing the nitrogenase substrate, acetylene. Ease of dissociation increases as oxygen is replaced by sulfur in the bridging unit. Electrocatalytic reduction products of acetylene consist primarily of C2H4 and C2H6 at low partial pressures, but C4H6, C4H8, and higher hydrocarbons predominate at higher pressures. The reduction of substrate is believed to occur by a Mo(III) catalyst adsorbed on the electrode surface. A mechanism is proposed for the e lectrocatalysis, and its relationship to the enzymic catalysis is discussed.
Show less - Date Issued
- 1977
- PURL
- http://purl.flvc.org/fcla/dt/13880
- Subject Headings
- Electrochemical analysis, Enzymatic analysis
- Format
- Document (PDF)
- Title
- ELECTROCHEMICALLY CATALYZED REDUCTION OF ACETYLENE BY A MOLYBDENUM - CYSTEINE COMPLEX.
- Creator
- LEDWITH, DEBRA ANN, Florida Atlantic University, Schultz, Franklin A., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
- Abstract/Description
-
The binuclear , di-mju-oxo-bridged molybdenum(V)-cysteine complex, Na2Mo2O4(cys)2, is frequently cited as a chemical model for nitrogenase enzyme. Coulometric reduction of Na2Mo2O4(cys)2 at the potential of Mo(V)--> Mo(III) reduction (-1. 35 to -1.50 V vs. SCE) in the presence o£ the nitrogenase substrate, acetylene, produces ethylene and ethane. Reduction of C2H2 does not occur in the absence of the molybdenumcysteine complex. The quantities of C2H4 and C2H6 produced are greater than the...
Show moreThe binuclear , di-mju-oxo-bridged molybdenum(V)-cysteine complex, Na2Mo2O4(cys)2, is frequently cited as a chemical model for nitrogenase enzyme. Coulometric reduction of Na2Mo2O4(cys)2 at the potential of Mo(V)--> Mo(III) reduction (-1. 35 to -1.50 V vs. SCE) in the presence o£ the nitrogenase substrate, acetylene, produces ethylene and ethane. Reduction of C2H2 does not occur in the absence of the molybdenumcysteine complex. The quantities of C2H4 and C2H6 produced are greater than the stoichiometric molybdenum content, hence the reduction is catalytic. Acetylene reduction is accompanied by catalytic hydrogen evolution at the surface of the mercury electrode. The rate of acetylene reduction and product yields vary with pH, applied electrode potential, concentration of Na2Mo2O4(cys)2, and the buffer salt. A mechanism for the catalytic process is proposed, and its relationship to chemical models for nitrogenase enzyme is discussed.
Show less - Date Issued
- 1975
- PURL
- http://purl.flvc.org/fcla/dt/13732
- Subject Headings
- Acetylene reduction assay, Molybdenum
- Format
- Document (PDF)
- Title
- ELECTROCHEMISTRY OF MONOMERIC MOLYBDENUM (VI)-, (V)-, (IV)- AND (III)- CATECHOL COMPLEXES IN AQUEOUS MEDIA.
- Creator
- CHARNEY, LYNN MARLENE., Florida Atlantic University, Schultz, Franklin A., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
- Abstract/Description
-
The electrochemical reduction of the cis-dioxo Mo(VI)-catechol complex, MoO2(cat2^2-, is investigated at a mercury electrode in pH 3.5-10 aqueous buffers utilizing the techniques of cyclic voltammetry and controlled potential coulometry. The reduction of MoO2(cat2^2- proceeds by successive two-proton, one-electron and two- proton, two-electron transfer steps which produce monomeric Mo(V) and Mo(III) species. At pH 3.5-7 the Mo(V) monomer is unstable and undergoes dimerization. The mechanism...
Show moreThe electrochemical reduction of the cis-dioxo Mo(VI)-catechol complex, MoO2(cat2^2-, is investigated at a mercury electrode in pH 3.5-10 aqueous buffers utilizing the techniques of cyclic voltammetry and controlled potential coulometry. The reduction of MoO2(cat2^2- proceeds by successive two-proton, one-electron and two- proton, two-electron transfer steps which produce monomeric Mo(V) and Mo(III) species. At pH 3.5-7 the Mo(V) monomer is unstable and undergoes dimerization. The mechanism of the dimerization reaction and the structure of the Mo(V) dimer produced are determined. At pH 7-10 the monomeric Mo (V) and 2- Mo(III) complexes produced by reduction of MoO2(cat2^2- are stable. A stable Mo(IV) species is obtained upon reoxidation of the Mo( III ) monomer. The epr spectrum of the monomeric Mo(V) complex and visible spectra of all oxidation states in alkaline solution arc reported.
Show less - Date Issued
- 1979
- PURL
- http://purl.flvc.org/fcla/dt/13979
- Subject Headings
- Electrochemistry
- Format
- Document (PDF)
- Title
- A SELF-RELIANT APPROACH TO CHEMICAL INSTRUMENTATION.
- Creator
- N'GURUMO, N'DETENGA., Florida Atlantic University, Schultz, Franklin A., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
- Abstract/Description
-
This thesis was planned to aid teachers in introducing chemical instrumentation in upper secondary or undergraduate chemistry courses. The problems encountered and solved in the construction of a single beam visible range spectrophotometer, conductance bridge, and polarimeter are described. Experiments were carried out with each instrument to demonstrate their efficiency as compared to commercial models. Pertinent theory on the operation of the instruments is included that can be easily...
Show moreThis thesis was planned to aid teachers in introducing chemical instrumentation in upper secondary or undergraduate chemistry courses. The problems encountered and solved in the construction of a single beam visible range spectrophotometer, conductance bridge, and polarimeter are described. Experiments were carried out with each instrument to demonstrate their efficiency as compared to commercial models. Pertinent theory on the operation of the instruments is included that can be easily understood by students on this educational level. Construction of the instruments is simple and inexpensive, which allows schools with limited budgets to introduce topics on instrumentation into the chemistry curriculum.
Show less - Date Issued
- 1974
- PURL
- http://purl.flvc.org/fcla/dt/13690
- Subject Headings
- Chemistry--Study and teaching (Secondary)
- Format
- Document (PDF)