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Microbial Community Resiliency and Role in Sea Level Rise
Title
Microbial Community Resiliency and Role in Sea Level Rise.
Creator
Klein, Christian, Esiobu, Nwadiuto, Office of Undergraduate Research and Inquiry
Abstract/Description
The interphase (littoral zone) of the Florida Everglades and surrounding marine environments are dominated by mangrove forests and mudflats that transition into freshwater communities. As salinity levels change, plant cover of the freshwater community will be adversely affected, but perhaps more importantly, their microbial symbionts responsible for their tolerance to high salinity will be impacted as well. Microbes respond quicker to changing environments and could provide an early warning...
Show moreThe interphase (littoral zone) of the Florida Everglades and surrounding marine environments are dominated by mangrove forests and mudflats that transition into freshwater communities. As salinity levels change, plant cover of the freshwater community will be adversely affected, but perhaps more importantly, their microbial symbionts responsible for their tolerance to high salinity will be impacted as well. Microbes respond quicker to changing environments and could provide an early warning of the loss of resiliency of the Florida coastline ecosystem. For 10 weeks, soil samples from Biscayne National Park will be placed in standardized mesocosms and subject to local conditions like sunlight, tides, plants, increased sea levels, higher salinities, and variant inundation. Standard and variant conditions will be employed on different mesocosms, and weekly DNA extractions of the various soil samples will be analyzed via metagenomic analysis, creating a library of the 16S rRNA community structure over the 10 week period.
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Date Issued
2017
PURL
http://purl.flvc.org/fau/fd/FA00005623
Subject Headings
College students --Research --United States.
Format
Document (PDF)
Impact of Vitamin C on Genistein induced apoptosis in treatment of prostate cancer cells
Title
Impact of Vitamin C on Genistein induced apoptosis in treatment of prostate cancer cells.
Creator
Famuyiwa, Toluleke, Boe, Andrew, Esiobu, Nwadiuto, Graduate College, Kumi-Diaka, James
Abstract/Description
Background: Prostate Cancer, in the absence of skin cancer, is the most prevalent type of cancer found in the male population. Reactive Oxygen Species (ROS) can promote cancer cell proliferation when they are at elevated levels. Vitamin C is a water-soluble antioxidant capable of inhibiting the formation of ROS. Genistein, an isoflavone found in plants, also possesses the ability to inhibit ROS formation. Objective To determine the potential therapeutic synergy between genistein and vitamin C...
Show moreBackground: Prostate Cancer, in the absence of skin cancer, is the most prevalent type of cancer found in the male population. Reactive Oxygen Species (ROS) can promote cancer cell proliferation when they are at elevated levels. Vitamin C is a water-soluble antioxidant capable of inhibiting the formation of ROS. Genistein, an isoflavone found in plants, also possesses the ability to inhibit ROS formation. Objective To determine the potential therapeutic synergy between genistein and vitamin C and investigate mechanism of action of genistein and/or vitamin C. Methods: Trypan blue assay was carried out to know the % of viable cells. Varying concentrations of genistein with a constant concentration of Vitamin C was used to treat LNCaP cells. After treatment of the cells with genistein and Vitamin C, MTT assay of the cancer cells was performed and absorbance read through an ELISA reader. This gives the values needed for interpreting cell viability after treatment. A statistical analysis performed to determine whether the obtained results are statistically significant. Results: The results obtained from our experiments are inconclusive with regards to the impact of Vitamin C on apoptotic cancer cell death following genistein treatment. However the combination of genistein and vitamin C was more efficient in tumor suppression than when the drugs were given separately. Conclusion: This study suggests that treatment of prostate cancer using genistein can be enhanced by adjuvant treatment with vitamin C. This study is of potential clinical success in reducing the cell death by necrosis.
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Date Issued
2015
PURL
http://purl.flvc.org/fau/fd/FA00005876
Format
Document (PDF)
A simple method for measuring antibiotic resistance in a community of bacteria
Title
A simple method for measuring antibiotic resistance in a community of bacteria.
Creator
Pelski, Ana, Samaroo, Sheynelle, Varughese, Vipin, Behr, Evalena, Browne, Trisha, Esiobu, Nwadiuto
Abstract/Description
Antibiotic resistant bacteria infiltrate drinking water, increase human exposure, and spread into clinical settings. Regulatory agencies need simple test for ARI to monitor environmental quality. This study is first to measure resistance in a consortium of bacteria, not pure cultures. Our rapid method – “In-the-zone” (ITZ) employs a) the size of zones of inhibition b) identity of bacteria in the zone c) number of resistant clones within zones of inhibition and d) type of antibiotic to compute...
Show moreAntibiotic resistant bacteria infiltrate drinking water, increase human exposure, and spread into clinical settings. Regulatory agencies need simple test for ARI to monitor environmental quality. This study is first to measure resistance in a consortium of bacteria, not pure cultures. Our rapid method – “In-the-zone” (ITZ) employs a) the size of zones of inhibition b) identity of bacteria in the zone c) number of resistant clones within zones of inhibition and d) type of antibiotic to compute ARI. Results can be obtained within 24 - 48 hrs with very little effort. This semester, we repeated the test and validated the weighting of various parameters. The ITZ assay yield consistent and reproducible data on zones of inhibition of bacteria community. The diameter of inhibition zones was the most important parameter distinguishing resistomes of varying ARI. Landfill, manured garden, and waste plant samples scored highest in antibiotic resistance index compared to beach sand.
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Date Issued
2014
PURL
http://purl.flvc.org/fau/fd/FA0005034
Subject Headings
College students --Research --United States.
Format
Document (PDF)
In search of environmental hotspots of antibiotic resistance
Title
In search of environmental hotspots of antibiotic resistance.
Creator
Desir, Hedson, Esiobu, Nwadiuto
Date Issued
2013-04-05
PURL
http://purl.flvc.org/fcla/dt/3361076
Subject Headings
Antibiotics resistance in microorganisms, Environmental hotspots
Format
Document (PDF)