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Bacterial Community Analysis of Soil Samples Beneath Decomposing Sus scrofa Carcasses
Title
Bacterial Community Analysis of Soil Samples Beneath Decomposing Sus scrofa Carcasses.
Creator
Brown, Spencer, Ebanks, Ryan, Frazier, Evelyn, Caruso, Joseph P., Charles E. Schmidt College of Science
Abstract/Description
The post-mortem interval (PMI) is the time elapsed from the time of death until the discovery of a decomposing body, which forensic scientists utilize to decipher how and when a death occurred. Much of decomposition is attributable to bacterial activity. Identifying a net change in bacterial composition throughout the PMI of a decomposing carcass helps to explain the mechanism of decomposition. Carcasses of four feral swine were placed in cages in direct sunlight, whereby soil samples were...
Show moreThe post-mortem interval (PMI) is the time elapsed from the time of death until the discovery of a decomposing body, which forensic scientists utilize to decipher how and when a death occurred. Much of decomposition is attributable to bacterial activity. Identifying a net change in bacterial composition throughout the PMI of a decomposing carcass helps to explain the mechanism of decomposition. Carcasses of four feral swine were placed in cages in direct sunlight, whereby soil samples were taken prior to and following seven days of decomposition. Bacterial DNA was then isolated from the soil samples and amplified via polymerase-chain reaction. Further metagenomic analysis was conducted by an outside agency using 16S ribosomal RNA (rRNA) amplicon sequencing to determine the composition of the bacterial community. A relationship between the stages of decomposition and the anatomy of bacterial communities involved was determined, which helps to elucidate the process of decomposition.
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Date Issued
2015
PURL
http://purl.flvc.org/fau/fd/FA00005178
Subject Headings
College students --Research --United States.
Format
Document (PDF)
Baseline Study for the Identification of Intestinal Parasites in Gopher Tortoises Found in Blazing Star and Pine Jog
Title
Baseline Study for the Identification of Intestinal Parasites in Gopher Tortoises Found in Blazing Star and Pine Jog.
Creator
Shanker, Rachel, Haizlett, Kent, Huffman, Jessica, Frazier, Evelyn, Caruso, Joseph P., Charles E. Schmidt College of Science
Abstract/Description
Gopher tortoise (Gopherus polyphemus) populations are diminishing in South Florida due to habitat fragmentation in urban sites. As a keystone species, gopher tortoises have important ecological roles. The goal of this study was to establish a baseline of intestinal parasites in two South Florida populations. One population resides in a fragmented and non-fire maintained site, Blazing Star, and the other in a fire maintained site, Pine Jog. Fire is a natural factor in these habitats reducing...
Show moreGopher tortoise (Gopherus polyphemus) populations are diminishing in South Florida due to habitat fragmentation in urban sites. As a keystone species, gopher tortoises have important ecological roles. The goal of this study was to establish a baseline of intestinal parasites in two South Florida populations. One population resides in a fragmented and non-fire maintained site, Blazing Star, and the other in a fire maintained site, Pine Jog. Fire is a natural factor in these habitats reducing canopy cover and killing parasite eggs. The lack of fire in Blazing Star can lead to overgrowth of vegetation, forcing tortoises to crowd where lower vegetation is found. We hypothesize that tortoises from Blazing Star will have a higher degree of intestinal parasites, when compared to those from Pine Jog due to potential crowding. Data is currently being collected, and may provide insight to improve future management and conservation practices.
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Date Issued
2015
PURL
http://purl.flvc.org/fau/fd/FA00005209
Subject Headings
College students --Research --United States.
Format
Document (PDF)
An identification and comparison of intestinal parasites found within Gopherus polyphemus at two differing Southeastern Florida habitats
Title
An identification and comparison of intestinal parasites found within Gopherus polyphemus at two differing Southeastern Florida habitats.
Creator
Huffman, Jessica, Frazier, Evelyn, Graduate College, Caruso, Joseph P.
Abstract/Description
Gopherus polyphemus is a keystone terrestrial reptile, currently threatened in Florida due to habitat destruction. Overcrowded populations are associated with higher infectious disease transmission. Parasite roles within wild tortoise populations are largely unknown, despite growing evidence they may pose significant health risks. There is a large gopher tortoise population in the fragmented, poorly maintained Florida Atlantic University Preserve (FAUP). A separate Martin County population is...
Show moreGopherus polyphemus is a keystone terrestrial reptile, currently threatened in Florida due to habitat destruction. Overcrowded populations are associated with higher infectious disease transmission. Parasite roles within wild tortoise populations are largely unknown, despite growing evidence they may pose significant health risks. There is a large gopher tortoise population in the fragmented, poorly maintained Florida Atlantic University Preserve (FAUP). A separate Martin County population is at Jonathan Dickinson State Park (JDSP) in a higher quality habitat. We hypothesized there would be higher intestinal parasite loads in FAUP tortoises than JDSP tortoises, due to overcrowding. Several methods were used to determine parasite species and infection intensities. Fecal flotations revealed moderate to high Strongyle and Hookworm intensities in FAUP tortoises; in contrast, low Hookworm and moderate Strongyle intensities were seen in JDSP tortoises. Comparing infection intensities to degree of crowding is important to optimize management strategies of these threatened species.
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Date Issued
2014
PURL
http://purl.flvc.org/fau/fd/FA00005150
Format
Document (PDF)
Comparative survey of hemoparasites in two south Florida gopher tortoise populations
Title
Comparative survey of hemoparasites in two south Florida gopher tortoise populations.
Creator
Elhassani, Dana, Caruso, Joseph P., Graduate College, Frazier, Evelyn
Abstract/Description
The gopher tortoise (Gopherus polyphemus) is a threatened keystone species of the Southeastern United States. Increasing urban encroachment and tortoise translocation increases the risk of disease transmission. Prior work at Florida Atlantic University suggested resident gopher tortoises had high tick burdens. To investigate ticks as potential disease vectors, two sites were examined. The Florida Atlantic University Preserve (FAUP); managed by mowing and herbicides and Jonathan Dickinson...
Show moreThe gopher tortoise (Gopherus polyphemus) is a threatened keystone species of the Southeastern United States. Increasing urban encroachment and tortoise translocation increases the risk of disease transmission. Prior work at Florida Atlantic University suggested resident gopher tortoises had high tick burdens. To investigate ticks as potential disease vectors, two sites were examined. The Florida Atlantic University Preserve (FAUP); managed by mowing and herbicides and Jonathan Dickinson State Park (JDSP); managed by fire. We hypothesized that gopher tortoises in the FAUP will be infected by heavier hemoparasite loads, potentially causing anemia, when compared to JDSP. To detect hemoparasites, blood was collected from tortoises at FAUP and JDSP. Blood smears revealed intracellular parasites and inclusions at both sites. Packed cell volumes were done to assess whether tortoises were anemic; results found tortoises were not anemic during that sampling period. This study will provide baseline data for blood-borne infections in two south Florida gopher tortoise populations.
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Date Issued
2014
PURL
http://purl.flvc.org/fau/fd/FA00005145
Format
Document (PDF)