Current Search: poster (x) » text (x) » FAU Undergraduate Student Research (x) » Zhang, Xing-Hai (x) » Justs, Adriana (x)
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Title
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Genetic Engineering of Tomato Plants Expressing β-Glucuronidasethrough Agrobacterium-mediated Transformation.
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Creator
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Justs, Adriana, Kaplan, Noah, Zhang, Xing-Hai, Office of Undergraduate Research and Inquiry
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Abstract/Description
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Advancements in biotechnology have allowed us to study genetics and plant physiology by engineering transgenic plants. For our research we transformed Micro-Tom, a tomato variety developed for use in genetic research, using Agrobacterium mediated transformation. Within a time span of fourteen weeks, we inserted two distinct plasmid constructs (pCAMBIA2301 and E1492). Plants have the unique ability to regenerate their tissue and we took advantage of this ability to regenerate the transgenic...
Show moreAdvancements in biotechnology have allowed us to study genetics and plant physiology by engineering transgenic plants. For our research we transformed Micro-Tom, a tomato variety developed for use in genetic research, using Agrobacterium mediated transformation. Within a time span of fourteen weeks, we inserted two distinct plasmid constructs (pCAMBIA2301 and E1492). Plants have the unique ability to regenerate their tissue and we took advantage of this ability to regenerate the transgenic plants with antibiotic selection. Approximately one third of the explants endured the infection process and fourteen of these survived in the presence of kanamycin. By the end of the fourteenth week, eleven out of our fourteen plantlets had fully developed roots but only four survived to maturity. After verification with PCR and qPCR, we found that we generated two transgenic plants. Here we describe all the methods and techniques used to achieve these compelling results.could be the potential cause of this neurodegenerative disease, will help elucidate the role of this amino acid in ALS.
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Date Issued
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2016
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PURL
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http://purl.flvc.org/fau/fd/FA00005578
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Subject Headings
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College students --Research --United States.
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Format
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Document (PDF)
