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- Title
- Use of real-time qPCR to quantify members of the unculturable heterotrophic bacterial community in a deep sea marine sponge, Vetulina sp.
- Creator
- Cassler, M., Peterson, C. L., Ledger, Angela, Pomponi, Shirley A., Wright, Amy E., Winegar, R., McCarthy, Peter J., Lopez, Jose V.
- Date Issued
- 2008
- PURL
- http://purl.flvc.org/FCLA/DT/2708323
- Subject Headings
- Microbiology --Research, Sponges --Microbiology, Marine biology --Caribbean Area
- Format
- Document (PDF)
- Title
- Reconstruction of Family-Level Phylogenetic Relationships within Demospongiae (Porifera) Using Nuclear Encoded Housekeeping Genes.
- Creator
- Hill, Malcolm S., Hill, April L., Lopez, Jose V., Peterson, Kevin J., Pomponi, Shirley A., Diaz, Maria Cristina, Thacker, Robert W., Adamska, Maja, Boury-Esnault, Nicole, Cárdenas, Paco, Chaves-Fonnegra, Andia, Danka, Elizabeth, De Laine, Bre-Onna, Formica, Dawn, Hajdu, Eduardo, Lobo-Hajdu, Gisele, Klontz, Sarah, Morrow, Christine C., Patel, Jignasa, Picton, Bernard, Pisani, Davide, Pohlmann, Deborah, Redmond, Niamh E., Reed, John K., Richey, Stacy, Riesgo, Ana, Rubin, Ewelina, Russell, Zach, Rützler, Klaus, Sperling, Erik A., di Stefano, Michael, Tarver, James E., Collins, Allen G., Lin, Senjie
- Abstract/Description
-
Background: Demosponges are challenging for phylogenetic systematics because of their plastic and relatively simple morphologies and many deep divergences between major clades. To improve understanding of the phylogenetic relationships within Demospongiae, we sequenced and analyzed seven nuclear housekeeping genes involved in a variety of cellular functions from a diverse group of sponges. Methodology/Principal Findings: We generated data from each of the four sponge classes (i.e., Calcarea,...
Show moreBackground: Demosponges are challenging for phylogenetic systematics because of their plastic and relatively simple morphologies and many deep divergences between major clades. To improve understanding of the phylogenetic relationships within Demospongiae, we sequenced and analyzed seven nuclear housekeeping genes involved in a variety of cellular functions from a diverse group of sponges. Methodology/Principal Findings: We generated data from each of the four sponge classes (i.e., Calcarea, Demospongiae, Hexactinellida, and Homoscleromorpha), but focused on family-level relationships within demosponges. With data for 21 newly sampled families, our Maximum Likelihood and Bayesian-based approaches recovered previously phylogenetically defined taxa: Keratosap, Myxospongiaep, Spongillidap, Haploscleromorphap (the marine haplosclerids) and Democlaviap. We found conflicting results concerning the relationships of Keratosap and Myxospongiaep to the remaining demosponges, but our results strongly supported a clade of Haploscleromorphap+Spongillidap+Democlaviap. In contrast to hypotheses based on mitochondrial genome and ribosomal data, nuclear housekeeping gene data suggested that freshwater sponges (Spongillidap) are sister to Haploscleromorphap rather than part of Democlaviap. Within Keratosap, we found equivocal results as to the monophyly of Dictyoceratida. Within Myxospongiaep, Chondrosida and Verongida were monophyletic. A wellsupported clade within Democlaviap, Tetractinellidap, composed of all sampled members of Astrophorina and Spirophorina (including the only lithistid in our analysis), was consistently revealed as the sister group to all other members of Democlaviap. Within Tetractinellidap, we did not recover monophyletic Astrophorina or Spirophorina. Our results also reaffirmed the monophyly of order Poecilosclerida (excluding Desmacellidae and Raspailiidae), and polyphyly of Hadromerida and Halichondrida. Conclusions/Significance: These results, using an independent nuclear gene set, confirmed many hypotheses based on ribosomal and/or mitochondrial genes, and they also identified clades with low statistical support or clades that conflicted with traditional morphological classification. Our results will serve as a basis for future exploration of these outstanding questions using more taxon- and gene-rich datasets.
Show less - Date Issued
- 2013-01-23
- PURL
- http://purl.flvc.org/fau/fd/FAUIR000044
- Format
- Citation
- Title
- Preliminary cruise report: Submersible and scuba collections in the coastal waters of Honduras, the Bay Islands: Biomedical and biodiversity research of the benthic communities with emphasis on the porifera, algae and gorgonacea.
- Creator
- Reed, John K., Pomponi, Shirley A., Hanisak, M. Dennis, Wright, Amy E., Janda, Kathleen E., Pitts, Tara P., Willoughby, Robin, Killday, K. B., Robertson, Lynn, Lopez, Jose V., Kelly-Borges, Klaus, Overdorf, Laura S., Monaghan, Pat
- Date Issued
- 1997-12-15
- PURL
- http://purl.flvc.org/fcla/dt/3359259
- Subject Headings
- Honduras, Biomedical Research, Biodiversity--Research
- Format
- Document (PDF)
- Title
- Molecular techniques reveal wide phyletic diversity of heterotrophic microbes associated with Discodermia spp. (Porifera: Demospongiae).
- Creator
- Lopez, Jose V., McCarthy, Peter J., Janda, Kathleen E., Willoughby, Robin, Pomponi, Shirley A.
- Date Issued
- 1999
- PURL
- http://purl.flvc.org/fau/fd/FA00007312
- Subject Headings
- Porifera, Demospongiae, Microbial diversity, Genes, rRNA, Symbiosis
- Format
- Document (PDF)
- Title
- In vitro production of marine-derived antitumor compounds.
- Creator
- Pomponi, Shirley A., Willoughby, Robin, Wright, Amy E., Pecorella, C., Sennett, Susan H., Lopez, Jose V., Samples, Gail A.
- Date Issued
- 1998
- PURL
- http://purl.flvc.org/fau/fd/FA00007238
- Subject Headings
- Antineoplastic agents, Bioactive compounds, Marine natural products, In vitro, Antitumor agents
- Format
- Document (PDF)
- Title
- HBMMD: an enhanced database of the microorganisms associated with deeper water marine invertebrates.
- Creator
- Gunasekera, Aravinda S., Sfanos, Karen S., Harmody, Dedra K., Pomponi, Shirley A., McCarthy, Peter J., Lopez, Jose V.
- Date Issued
- 2004
- PURL
- http://purl.flvc.org/FCLA/DT/2796029
- Subject Headings
- Marine microbiology, Microorganisms, Marine invertebrates, Bioactive compounds, Sponges
- Format
- Document (PDF)
- Title
- Characterization of genetic markers for in vitro cell line identification of the marine sponge, Axinella corrugata.
- Creator
- Lopez, Jose V., Peterson, C. L., Willoughby, Robin, Wright, Amy E., Enright, E., Zoladz, S., Pomponi, Shirley A.
- Date Issued
- 2002
- PURL
- http://purl.flvc.org/FCLA/DT/3355156
- Subject Headings
- Sponges--Research, Marine invertebrates, Genetic markers
- Format
- Document (PDF)
- Title
- A molecular systematic survey of sponge derived microbes.
- Creator
- Sandell, K., Peterson, C. L., Harmody, Dedra K., McCarthy, Peter J., Pomponi, Shirley A., Lopez, Jose V.
- Date Issued
- 2004
- PURL
- http://purl.flvc.org/fau/fd/FA00007425
- Subject Headings
- Sponges, Marine microbiology, Symbiosis, Polymorphism, Restriction Fragment Length, DNA Fingerprinting
- Format
- Document (PDF)
- Title
- A molecular systematic survey of cultured microbial associates of deep-water marine invertebrates.
- Creator
- Sfanos, Karen S., Harmody, Dedra K., Dang, Phat, Ledger, Angela, Pomponi, Shirley A., McCarthy, Peter J., Lopez, Jose V.
- Date Issued
- 2004
- PURL
- http://purl.flvc.org/FCLA/DT/2827820
- Subject Headings
- Molecular microbiology, Microbial ecology, Marine organisms, Microorganisms, Sponges
- Format
- Document (PDF)