Current Search: Sponges (x) » Marine pharmacology (x) » Graduate College (x)
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Title
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Effects of stressors on differential gene expression and secondary metabolites by Axinella corrugata.
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Creator
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Grima, Jennifer., Charles E. Schmidt College of Science, Department of Biological Sciences
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Abstract/Description
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Sponges are an important source of bioactive marine natural products, or secondary metabolites. The common Caribbean reef sponge, Axinella corrugata, produces an antitumor and antibacterial chemical, stevensine. This study determined whether environmental stressors, such as elevated temperature and exposure to Amphibalanus amphitrite larvae, affect the production of stevensine by A.corrugata and if the stressors caused A.corrugata to exhibit differential gene expression. Temperature stress...
Show moreSponges are an important source of bioactive marine natural products, or secondary metabolites. The common Caribbean reef sponge, Axinella corrugata, produces an antitumor and antibacterial chemical, stevensine. This study determined whether environmental stressors, such as elevated temperature and exposure to Amphibalanus amphitrite larvae, affect the production of stevensine by A.corrugata and if the stressors caused A.corrugata to exhibit differential gene expression. Temperature stress resulted in no significant change in the production of stevensine; only two genes were significantly differentially expressed, including hsp70. Larval stressed resulted in increased production of stevensine and significant differential gene expression (more than seventy genes). This study suggests that A.corrugata may be resilient to elevations in temperature and that one of stevensine's roles in nature is as an antifoulant.
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Date Issued
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2013
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PURL
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http://purl.flvc.org/fcla/dt/3360781
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Subject Headings
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Axinellida, Sponges, Marine pharmacology, Adaptation (Biology), Gene expression, Genetic regulation, Stress (Physiology), Ecophysiology
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Format
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Document (PDF)
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Title
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Farming sponges for chemicals with pharmaceutical potential.
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Creator
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Duckworth, Alan R., Harbor Branch Oceanographic Institute
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Date Issued
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2001
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PURL
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http://purl.flvc.org/FCLA/DT/3352965
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Subject Headings
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Sponges, Metabolites, Sponges--Biotechnology, Marine metabolites, Marine pharmacology
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Format
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Document (PDF)
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Title
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In vitro biosynthetic studies of stevensine, a natural product from the sponge Teichaxinella morchella.
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Creator
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Andrade, Paul., Florida Atlantic University, Kerr, Russell G.
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Abstract/Description
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Stevensine is a biologically active secondary metabolite produced by several marine sponges, including Teichaxinella morchella. Two in vitro methods were used to investigate the biosynthetic origin of stevensine in the sponge T. morchella. A cell-free enzyme extract was developed to test for the incorporation of the potential radiolabeled metabolic precursors histidine, arginine, ornithine, and proline. Secondly, cell cultures of T. morchella were used to determine the biosynthetic origin of...
Show moreStevensine is a biologically active secondary metabolite produced by several marine sponges, including Teichaxinella morchella. Two in vitro methods were used to investigate the biosynthetic origin of stevensine in the sponge T. morchella. A cell-free enzyme extract was developed to test for the incorporation of the potential radiolabeled metabolic precursors histidine, arginine, ornithine, and proline. Secondly, cell cultures of T. morchella were used to determine the biosynthetic origin of stevensine from the radiolabeled amino acids histidine, arginine, ornithine, and proline. Histidine and ornithine/proline were converted to stevensine in the cell culture system. This represents the first study involving the use of cell cultures of a marine invertebrate to investigate the biosynthesis of a biologically active natural product. An understanding of the biosynthetic process leading to the production of this compound in the source organism could lead to the development of more efficient and environmentally safer production methods.
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Date Issued
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1999
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PURL
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http://purl.flvc.org/fcla/dt/15620
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Subject Headings
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Sponges, Marine biotechnology, Marine pharmacology
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Format
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Document (PDF)
