Current Search: Department of Ocean and Mechanical Engineering (x) » Elishakoff, Isaac (x)
View All Items
Pages
- Title
- 2006-2007 Program Review Mechanical Engineering.
- Creator
- Florida Atlantic University, Department of Ocean and Mechanical Engineering, College of Engineering and Computer Science
- Abstract/Description
-
Florida Atlantic University Departmental Dashboard Indicators. Department program reviews for College of Engineering and Computer Science, Florida Atlantic University.
- Date Issued
- 2006-2007
- PURL
- http://purl.flvc.org/fau/fd/FA00007724
- Subject Headings
- Florida Atlantic University -- History
- Format
- Document (PDF)
- Title
- 2006-2007 Program Review Ocean Engineering.
- Creator
- Florida Atlantic University, Department of Ocean and Mechanical Engineering, College of Engineering and Computer Science
- Abstract/Description
-
Florida Atlantic University Departmental Dashboard Indicators. Department program reviews for College of Engineering and Computer Science, Florida Atlantic University.
- Date Issued
- 2006-2007
- PURL
- http://purl.flvc.org/fau/fd/FA00007725
- Subject Headings
- Florida Atlantic University -- History
- Format
- Document (PDF)
- Title
- 2009-2010 Program Review Ocean and Mechanical Engineering.
- Creator
- Florida Atlantic University, Department of Ocean and Mechanical Engineering, College of Engineering and Computer Science
- Abstract/Description
-
Florida Atlantic University Departmental Dashboard Indicators. Department program reviews for College of Engineering and Computer Science, Florida Atlantic University.
- Date Issued
- 2009-2010
- PURL
- http://purl.flvc.org/fau/fd/FA00007728
- Subject Headings
- Florida Atlantic University -- History
- Format
- Document (PDF)
- Title
- 2010-2011 Program Review Ocean and Mechanical Engineering.
- Creator
- Florida Atlantic University, Department of Ocean and Mechanical Engineering, College of Engineering and Computer Science
- Abstract/Description
-
Florida Atlantic University Departmental Dashboard Indicators. Department program reviews for College of Engineering and Computer Science, Florida Atlantic University.
- Date Issued
- 2010-2011
- PURL
- http://purl.flvc.org/fau/fd/FA00007731
- Subject Headings
- Florida Atlantic University -- History
- Format
- Document (PDF)
- Title
- 2012-2013 Program Review Ocean and Mechanical Engineering.
- Creator
- Florida Atlantic University, Department of Ocean and Mechanical Engineering, College of Engineering and Computer Science
- Abstract/Description
-
Florida Atlantic University Departmental Dashboard Indicators. Department program reviews for College of Engineering and Computer Science, Florida Atlantic University.
- Date Issued
- 2012-2013
- PURL
- http://purl.flvc.org/fau/fd/FA00007734
- Subject Headings
- Florida Atlantic University -- History
- Format
- Document (PDF)
- Title
- 2013-2014 Program Review Ocean and Mechanical Engineering.
- Creator
- Florida Atlantic University, Department of Ocean and Mechanical Engineering, College of Engineering and Computer Science
- Abstract/Description
-
Florida Atlantic University Departmental Dashboard Indicators. Department program reviews for College of Engineering and Computer Science, Florida Atlantic University.
- Date Issued
- 2013-2014
- PURL
- http://purl.flvc.org/fau/fd/FA00007737
- Subject Headings
- Florida Atlantic University -- History
- Format
- Document (PDF)
- Title
- 2014-2015 Program Review Ocean and Mechanical Engineering.
- Creator
- Florida Atlantic University, Department of Ocean and Mechanical Engineering, College of Engineering and Computer Science
- Abstract/Description
-
Florida Atlantic University Departmental Dashboard Indicators. Department program reviews for College of Engineering and Computer Science, Florida Atlantic University.
- Date Issued
- 2014-2015
- PURL
- http://purl.flvc.org/fau/fd/FA00007740
- Subject Headings
- Florida Atlantic University -- History
- Format
- Document (PDF)
- Title
- 2015-2016 Program Review Ocean and Mechanical Engineering.
- Creator
- Florida Atlantic University, Department of Ocean and Mechanical Engineering, College of Engineering and Computer Science
- Abstract/Description
-
Florida Atlantic University Departmental Dashboard Indicators. Department program reviews for College of Engineering and Computer Science, Florida Atlantic University.
- Date Issued
- 2015-2016
- PURL
- http://purl.flvc.org/fau/fd/FA00007743
- Subject Headings
- Florida Atlantic University -- History
- Format
- Document (PDF)
- Title
- 2016-2017 Program Review Ocean and Mechanical Engineering.
- Creator
- Florida Atlantic University, Department of Ocean and Mechanical Engineering, College of Engineering and Computer Science
- Abstract/Description
-
Florida Atlantic University Departmental Dashboard Indicators. Department program reviews for College of Engineering and Computer Science, Florida Atlantic University.
- Date Issued
- 2016-2017
- PURL
- http://purl.flvc.org/fau/fd/FA00007746
- Subject Headings
- Florida Atlantic University -- History
- Format
- Document (PDF)
- Title
- 3D-Printed Flexible Polylactic Acid/ Thermoplatic Polyurethane (PLA/TPU) Stents for Esophageal Malignancies.
- Creator
- Lin, Maohua, Kang, Yunqing, Tsai, Chi-Tay, Florida Atlantic University, College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
- Abstract/Description
-
Palliation therapy for dysphagia using esophageal stents is the current treatment of choice for those patients with inoperable esophageal malignancies. However, the stents currently used in the clinical setting, regardless of the type of metal mesh or plastic mesh stents (covered/uncovered), may cause complications, such as tumor ingrowth and stent migration into the stomach. Furthermore, metal mesh stents have limited capacities for loading anti-cancer drugs. To effectively reduce/overcome...
Show morePalliation therapy for dysphagia using esophageal stents is the current treatment of choice for those patients with inoperable esophageal malignancies. However, the stents currently used in the clinical setting, regardless of the type of metal mesh or plastic mesh stents (covered/uncovered), may cause complications, such as tumor ingrowth and stent migration into the stomach. Furthermore, metal mesh stents have limited capacities for loading anti-cancer drugs. To effectively reduce/overcome those complications and enhance the efficacy of drug release, we designed and 3D-printed a tubular, flexible polymer stent with spirals, and then load anti-cancer drug, paclitaxel, on the stent for drug release. Non- spiral 3D-printed tubular and mesh polymer stents served as controls. The self-expansion and anti migration properties, cytotoxicity, drug release profile, and cancer cell inhibition of the 3D-printed stent were fully characterized. Results showed the self-expansion force of the 3D-printed polymer stent with spirals was slightly higher than the stent without spirals. The anti-migration force of the 3D-printed stent with spirals was significantly higher than the anti-migration force of a non-spiral stent. Furthermore, the stent with spirals significantly decreased the migration distance compared to the migration distance of the non-spiral 3D-printed polymer stent. The in vitro cytotoxicity of the new stent was examined through the viability test of human esophagus epithelial cells, and results indicated that the polymer stent does not have any cytotoxicity. The results of in vitro cell viability of esophageal cancer cells further indicated that the paclitaxel in the spiral stent treated esophageal cancer cells much more efficiently than that in the mesh stent. Furthermore, the results of the in vitro drug release profile and drug permeation showed that the dense tubular drug-loaded stent could efficiently be delivered more paclitaxel through the esophageal mucosa/submucosa layers in a unidirectional way than mesh stent that delivered less paclitaxel to the esophageal mucosa/submucosa but more to the lumen. In summary, these results showed that the 3D-printed dense polymer stent with spirals has promising potential to treat esophageal malignancies.
Show less - Date Issued
- 2019
- PURL
- http://purl.flvc.org/fau/fd/FA00013230
- Subject Headings
- Paclitaxel, Stents, Esophageal Neoplasms, 3-D printing, Polymers in medicine
- Format
- Document (PDF)
- Title
- A Computational Analysis of Bio-Inspired Modified Boundary Layers for Acoustic Pressure Shielding in A Turbulent Wall Jet.
- Creator
- Gonzalez, Alexander J., Glegg, Stewart, Florida Atlantic University, College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
- Abstract/Description
-
Surface pressure fluctuations developed by turbulent flow within a boundary layer is a major cause of flow noise from a body and an issue which reveals itself over a wide range of engineering applications. Modified boundary layers (MBLs) inspired by the down coat of an owl’s wing has shown to reduce the acoustic effects caused by flow noise. This thesis investigates the mechanisms that modified boundary layers can provide for reducing the surface pressure fluctuations in a boundary layer....
Show moreSurface pressure fluctuations developed by turbulent flow within a boundary layer is a major cause of flow noise from a body and an issue which reveals itself over a wide range of engineering applications. Modified boundary layers (MBLs) inspired by the down coat of an owl’s wing has shown to reduce the acoustic effects caused by flow noise. This thesis investigates the mechanisms that modified boundary layers can provide for reducing the surface pressure fluctuations in a boundary layer. This study analyzes various types of MBLs in a wall jet wind tunnel through computational fluid dynamics and numerical surface pressure spectrum predictions. A novel surface pressure fluctuation spectrum model is developed for use in a wall jet boundary layer and demonstrates high accuracy over a range of Reynolds numbers. Non-dimensional parameters which define the MBL’s geometry and flow environment were found to have a key role in optimizing the acoustic performance.
Show less - Date Issued
- 2019
- PURL
- http://purl.flvc.org/fau/fd/FA00013209
- Subject Headings
- Turbulent flow, Turbulent boundary layer, Computational fluid dynamics, Wall jets
- Format
- Document (PDF)
- Title
- A Deep Learning Approach To Target Recognition In Side-Scan Sonar Imagery.
- Creator
- Einsidler, Dylan, Dhanak, Manhar R., Florida Atlantic University, College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
- Abstract/Description
-
Automatic target recognition capabilities in autonomous underwater vehicles has been a daunting task, largely due to the noisy nature of sonar imagery and due to the lack of publicly available sonar data. Machine learning techniques have made great strides in tackling this feat, although not much research has been done regarding deep learning techniques for side-scan sonar imagery. Here, a state-of-the-art deep learning object detection method is adapted for side-scan sonar imagery, with...
Show moreAutomatic target recognition capabilities in autonomous underwater vehicles has been a daunting task, largely due to the noisy nature of sonar imagery and due to the lack of publicly available sonar data. Machine learning techniques have made great strides in tackling this feat, although not much research has been done regarding deep learning techniques for side-scan sonar imagery. Here, a state-of-the-art deep learning object detection method is adapted for side-scan sonar imagery, with results supporting a simple yet robust method to detect objects/anomalies along the seabed. A systematic procedure was employed in transfer learning a pre-trained convolutional neural network in order to learn the pixel-intensity based features of seafloor anomalies in sonar images. Using this process, newly trained convolutional neural network models were produced using relatively small training datasets and tested to show reasonably accurate anomaly detection and classification with little to no false alarms.
Show less - Date Issued
- 2018
- PURL
- http://purl.flvc.org/fau/fd/FA00013025
- Subject Headings
- Deep learning, Sidescan sonar, Underwater vision
- Format
- Document (PDF)
- Title
- A Design Concept for Launch and Recovery of REMUS 100 AUV from WAM-V 16 USV.
- Creator
- Palmaccio, Brendan, Dhanak, Manhar, Florida Atlantic University, Department of Ocean and Mechanical Engineering, College of Engineering and Computer Science
- Abstract/Description
-
In this thesis, feasibility of a concept for launch and recovery of the Remus AUV from WAM-V USV is investigated. A modular recovery system which can be added to the WAM-V payload tray was designed, and based on a review of previous literature a CONOPS was developed for the launch and recovery process. The first phase of the CONOPS, which pertains to the position of the REMUS initially on the free surface prior to sling engagement is simulated using ANSYS AQWA. Preprocessing for the...
Show moreIn this thesis, feasibility of a concept for launch and recovery of the Remus AUV from WAM-V USV is investigated. A modular recovery system which can be added to the WAM-V payload tray was designed, and based on a review of previous literature a CONOPS was developed for the launch and recovery process. The first phase of the CONOPS, which pertains to the position of the REMUS initially on the free surface prior to sling engagement is simulated using ANSYS AQWA. Preprocessing for the simulation involved simplification of the model in ANSYS SpaceClaim to achieve a proper mesh as well as theoretical calculations of the input parameters for wave environment and point masses etc. The simulation was evaluated by taking into consideration two wave environment scenarios: beam sea’s (-90 degrees) and head seas (0 degrees). The wave environment was based on a linear frequency range for the waves which considered wavelengths that correspond to half and double the length of the WAM-V 16’. The significance of the simulation is characterized through identifying the ideal direction and wave frequency range for recovery based on the RAOs of the two vehicles.
Show less - Date Issued
- 2023
- PURL
- http://purl.flvc.org/fau/fd/FA00014216
- Subject Headings
- Autonomous underwater vehicles--Design and construction, Marine engineering, Littoral combat ships
- Format
- Document (PDF)
- Title
- A direct thrust measurement system for a waterjet propelled, free running USV.
- Creator
- Grimes, John A., von Ellenrieder, Karl, College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
- Abstract/Description
-
The relationship between cross-flow at a waterjet inlet and delivered thrust is not fully understood. A direct thrust measurement system was designed for a waterjet propelled, free running USV. To induce sway velocity at the waterjet inlet, which was considered equivalent to the cross flow, circles of varying radii were performed at Reynolds Numbers between 3.48 x 106 and 8.7 x 106 and radii from 2.7 to 6.3 boat lengths. Sway velocities were less than twenty percent of mean forward speed with...
Show moreThe relationship between cross-flow at a waterjet inlet and delivered thrust is not fully understood. A direct thrust measurement system was designed for a waterjet propelled, free running USV. To induce sway velocity at the waterjet inlet, which was considered equivalent to the cross flow, circles of varying radii were performed at Reynolds Numbers between 3.48 x 106 and 8.7 x 106 and radii from 2.7 to 6.3 boat lengths. Sway velocities were less than twenty percent of mean forward speed with slip angles that were less than 20°. Thrust Loading Coefficients were compared to sway as a percent of forward speed. In small radius turns, no relationship was seen, while in larger radius turns, peaks of sway velocity corresponded with drops in thrust, but this was determined to be caused by reduced vehicle yaw in these intervals . Decoupling of thrust and yaw rate is recommended for future research.
Show less - Date Issued
- 2013
- PURL
- http://purl.flvc.org/fau/fd/FA0004023
- Subject Headings
- Oceanographic instruments -- Evaluation, Propellers, Ship propulsion, Ships -- Hydrodynamics, Stability of ships
- Format
- Document (PDF)
- Title
- A high-level fuzzy logic guidance system for an unmanned surface vehicle (USV) tasked to perform an autonomous launch and recovery (ALR) of an unmanned underwater vehicle (UUV).
- Creator
- Pearson, David, An, Pak-Cheung, Florida Atlantic University, College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
- Abstract/Description
-
There have been much technological advances and research in Unmanned Surface Vehicles (USV) as a support and delivery platform for Autonomous/Unmanned Underwater Vehicles (AUV/UUV). Advantages include extending underwater search and survey operations time and reach, improving underwater positioning and mission awareness, in addition to minimizing the costs and risks associated with similar manned vessel operations. The objective of this thesis is to present the design and development a high...
Show moreThere have been much technological advances and research in Unmanned Surface Vehicles (USV) as a support and delivery platform for Autonomous/Unmanned Underwater Vehicles (AUV/UUV). Advantages include extending underwater search and survey operations time and reach, improving underwater positioning and mission awareness, in addition to minimizing the costs and risks associated with similar manned vessel operations. The objective of this thesis is to present the design and development a high-level fuzzy logic guidance controller for a WAM-V 14 USV in order to autonomously launch and recover a REMUS 100 AUV. The approach to meeting this objective is to develop ability for the USV to intercept and rendezvous with an AUV that is in transit in order to maximize the probability of a final mobile docking maneuver. Specifically, a fuzzy logic Rendezvous Docking controller has been developed that generates Waypoint-Heading goals for the USV to minimize the cross-track errors between the USV and AUV. A subsequent fuzzy logic Waypoint-Heading controller has been developed to provide the desired heading and speed commands to the low-level controller given the Waypoint-Heading goals. High-level mission control has been extensively simulated using Matlab and partially characterized in real-time during testing. Detailed simulation, experimental results and findings will be reported in this paper.
Show less - Date Issued
- 2014
- PURL
- http://purl.flvc.org/fau/fd/FA00004315, http://purl.flvc.org/fau/fd/FA00004315
- Subject Headings
- Adaptive signal processing, Fuzzy sets, Fuzzy systems, Nonlinear control theory, Oceanographic submersibles -- Automatic control, Submersibles -- Control systems, Underwater acoustic telemetry
- Format
- Document (PDF)
- Title
- A Hybrid System for Simulation of Athletic Activities Related to Lower Extremity Biomechanics.
- Creator
- Trepeck, Cameron, Hashemi, Javad, Florida Atlantic University, College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
- Abstract/Description
-
In this dissertation, the design and development of a hybrid robotic system that simulates dynamic biomechanical tasks of the lower extremity with emphasis on knee and hip joints are presented. The hybrid system utilizes a mechanical hip and a cadaveric knee/ankle component and can accelerate the whole complex towards the ground. This system is used to simulate complex athletic movements such as landing from a jump at various anatomical orientations of the lower extremity with muscle action....
Show moreIn this dissertation, the design and development of a hybrid robotic system that simulates dynamic biomechanical tasks of the lower extremity with emphasis on knee and hip joints are presented. The hybrid system utilizes a mechanical hip and a cadaveric knee/ankle component and can accelerate the whole complex towards the ground. This system is used to simulate complex athletic movements such as landing from a jump at various anatomical orientations of the lower extremity with muscle action. The dynamic response of the lower extremity is monitored and analyzed during impulsive contact between the ground and the cadaveric leg. The cadaveric knee is instrumented to measure strain of the Anterior Cruciate Ligament (ACL) during simulated high impact sports activities. The mechanical hip allows various kinematics of the hip including flexion as well as abduction. In addition to the flexion and abduction of the mechanical hip, the controlled flexion and extension of the cadaveric knee allows for simulation of complex tasks such as landing from a jump. A large number of tests were performed at various anatomical positions utilizing this device to simulate landing from a jump. ACL strain was measured during these tasks using a Differential Variance Resistance Transducer (DVRT). Ground Reaction Force and muscle forces were measured and monitored using AmCell load cells recorded using the LabView software. one-inch and 6-inch jump landing heights were used for all the simulations. The tests were performed at differing angles of hip flexion (0°, 30°, 45°, 60°) and at two different ankle positions. Plantar flexion and flat-footed landing conditions were simulated and compared in all degrees of hip flexion. These tests were repeated with and without hip abduction in order to study the effects of these landing positions on ACL strain. Hip flexion was found to effect ACL strain: as angle of hip flexion increases, ACL strain decreases. This occurred in both abducted and non-abducted hip positions. Ankle landing position had an effect only in small drop heights, while hip abduction had an effect in large drops. Future tests must be completed to further study these effects. These studies showed that the robotic system can simulate dynamic tasks, apply muscle forces, and move the cadaveric tissue in three dimensional biomechanical positions.
Show less - Date Issued
- 2017
- PURL
- http://purl.flvc.org/fau/fd/FA00004898, http://purl.flvc.org/fau/fd/FA00004898
- Subject Headings
- Musculoskeletal system--Wounds and injuries., Musculoskeletal system--Mechanical properties., Biomechanics--Computer simulation., Human mechanics., Artificial joints.
- Format
- Document (PDF)
- Title
- A lime softening system for individual-household use powered by solar energy.
- Creator
- Slater-Williams, Alexandria, Abtahi, Homayoon, Florida Atlantic University, College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
- Abstract/Description
-
A study conducted by the World Health Organization and United Nations Children's Fund, concluded that approximately 768 million people worldwide are not receiving sanitary drinking water suitable for consumption. While there are many water treatment methods, lime softening proves to be one of the more effective approaches as it removes a wide variety of harmful compounds including arsenic, lead, mercury, and cadmium under the correct conditions. The greatest issues with lime softening on a...
Show moreA study conducted by the World Health Organization and United Nations Children's Fund, concluded that approximately 768 million people worldwide are not receiving sanitary drinking water suitable for consumption. While there are many water treatment methods, lime softening proves to be one of the more effective approaches as it removes a wide variety of harmful compounds including arsenic, lead, mercury, and cadmium under the correct conditions. The greatest issues with lime softening on a smaller scale include the complexity of the chemistry and need for monitoring. By designing the system for groundwater sources and with a smaller capacity, this thesis hoped to reduce the level of monitoring, chemical expertise, and cost needed for operation. While promising results occurred in the removal of arsenic and total hardness, this project was unable to obtain consistent results and final water samples with pH values between the recommended standard of 6.5 to 8.
Show less - Date Issued
- 2015
- PURL
- http://purl.flvc.org/fau/fd/FA00004412, http://purl.flvc.org/fau/fd/FA00004412
- Subject Headings
- Solar energy -- Economic aspects, Water -- Purification, Water -- Purification Water -- Softening, Water quality management, Water supply -- Design and construction, Water supply engineering
- Format
- Document (PDF)
- Title
- A Low-Level USV Controller Incorporating an Environmental Disturbance Observer.
- Creator
- Diddams, Michael Albert, Dhanak, Manhar R., Florida Atlantic University, College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
- Abstract/Description
-
Modeling, system identification and controller design for a 16’ catamaran is described with the objective of enhanced operation in the presence of environmental disturbances including wind, waves and current. The vehicle is fully-actuated in surge, sway and yaw degrees of freedom. Analytical and experimental system identification is carried out to create a numerical model of the vehicle. A composite system of a Multiinput multi-output Proportional-Derivative (PD) controller and a nonlinear...
Show moreModeling, system identification and controller design for a 16’ catamaran is described with the objective of enhanced operation in the presence of environmental disturbances including wind, waves and current. The vehicle is fully-actuated in surge, sway and yaw degrees of freedom. Analytical and experimental system identification is carried out to create a numerical model of the vehicle. A composite system of a Multiinput multi-output Proportional-Derivative (PD) controller and a nonlinear disturbance observer is used for station-keeping and transiting modes of operation. A waypoint transiting algorithm is developed to output heading and cross-track error from vehicle position and waypoints. A control allocation method is designed to lower azimuthing frequency and incorporate angle saturation and rate limits. Validation is achieved with improvement in simulation with the addition of the nonlinear observer.
Show less - Date Issued
- 2018
- PURL
- http://purl.flvc.org/fau/fd/FA00013022
- Subject Headings
- Unmanned surface vehicles, Environmental disturbances, Catamarans--Design and construction
- Format
- Document (PDF)
- Title
- A Modeling Study on The Effects of Seagrass Beds on the Hydrodynamics in the Indian River Lagoon.
- Creator
- Habib, MD Ahsan, Jiang, Mingshun, Florida Atlantic University, College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
- Abstract/Description
-
Seagrass is a key stone component for the Indian River Lagoon (IRL) ecosystem, and therefore it is an important topic for many studies in the lagoon. This study focuses on the effects of seagrass beds on the hydrodynamics in the IRL. A hydrodynamic model based on the Delft3D modeling system has been developed for the southern IRL including the St. Lucie estuary, Ft. Pierce and St. Lucie Inlets, and adjacent coastal waters. The model is driven by freshwater inputs from the watershed, tides,...
Show moreSeagrass is a key stone component for the Indian River Lagoon (IRL) ecosystem, and therefore it is an important topic for many studies in the lagoon. This study focuses on the effects of seagrass beds on the hydrodynamics in the IRL. A hydrodynamic model based on the Delft3D modeling system has been developed for the southern IRL including the St. Lucie estuary, Ft. Pierce and St. Lucie Inlets, and adjacent coastal waters. The model is driven by freshwater inputs from the watershed, tides, meteorological forcing, and oceanic boundary forcing. The model has been systematically calibrated through a series of numerical experiments for key parameters, particularly the bottom roughness, and configuration including heat flux formulation and bottom bathymetry. The model skills were evaluated with quantitative metrics (point-to-point correlation, root-mean-square difference, and mean bias) to gauge the agreements between model and data for key variables including temperature, salinity, and currents. A three-year (2013-2015) simulation has been performed, and the results have been validated with available data including observations at HBOI Land-Ocean Biogeochemistry Observatory (LOBO) stations and in situ measurements from various sources. The validated model is then used to investigate the effects of 1) model vertical resolution (total number of model vertical layers), 2) spatial variability of surface winds, and 3) seagrass beds on the simulated hydrodynamics. The study focuses on the vicinity of Ft. Pierce Inlet, where significant seagrass coverage can be found. A series of numerical experiments were performed with a combination of different configurations. Overall, the experiment with 2-dimensional (2-D) winds, ten vertical layers and incorporating seagrass provided the most satisfactory outcomes. Overall, both vertical resolution and spatial variability of surface winds affect significantly the model results. In particular, increasing vertical resolution improves model prediction of temperature, salinity and currents. Similarly, the model with 2-D winds yields more realistic results than the model forced by 0-D winds. The seagrass beds have significant effects on the model results, particularly the tidal and sub-tidal currents. In general, model results show that both tidal and sub-tidal currents are much weaker due to increase bottom friction from seagrass. For tidal currents, the strongest impacts lie in the main channel (inter-coastal waterway) and western part of the lagoon, where strong tidal currents can be found. Inclusion of seagrass in the model also improves the simulation of sub-tidal currents. Seagrass beds also affect model temperature and salinity including strengthening vertical stratification. In general, seagrass effects vary over time, particularly tidal cycle with stronger effects seen in flood and ebb tides, and seasonal cycle with stronger effects in the summer than in winter.
Show less - Date Issued
- 2016
- PURL
- http://purl.flvc.org/fau/fd/FA00004774, http://purl.flvc.org/fau/fd/FA00004774
- Subject Headings
- Turtle grass--Environmental aspects., Seagrasses--Ecology., Grassed waterways., Wave resistance (Hydrodynamics), Wetland ecology., Estuarine ecology., Estuarine restoration., Coastal zone management.
- Format
- Document (PDF)
- Title
- A NEW DECELLULARIZED MATRIX FOR ENGINEERING AN ESOPHAGEAL CANCER MODEL IN A PERFUSION BIOREACTOR.
- Creator
- Brennan, Jordan Michael, Kang, Yunqing, Florida Atlantic University, Department of Ocean and Mechanical Engineering, College of Engineering and Computer Science
- Abstract/Description
-
The lack of physiologically relevant human esophageal cancer models has as a result that many esophageal cancer studies are encountering major bottleneck challenges in achieving breakthrough progress. To address the issue, here a 3D esophageal tumor tissue model was engineered using a biomimetic decellularized esophageal matrix in a customized bioreactor. To obtain a biomimetic esophageal matrix, a detergent-free, rapid decellularization method was developed to decellularize porcine esophagus...
Show moreThe lack of physiologically relevant human esophageal cancer models has as a result that many esophageal cancer studies are encountering major bottleneck challenges in achieving breakthrough progress. To address the issue, here a 3D esophageal tumor tissue model was engineered using a biomimetic decellularized esophageal matrix in a customized bioreactor. To obtain a biomimetic esophageal matrix, a detergent-free, rapid decellularization method was developed to decellularize porcine esophagus. The decellularized esophageal matrix (DEM) was characterized and the DEM was utilized for the growth of esophageal cancer cell KYSE30 in well plates and the bioreactor. Then the expression of cancerrelated markers of KYSE30 cells was analyzed and compared with formalin-fixed, paraffin-embedded (FFPE) esophageal squamous cell carcinoma (ESCC) tissue biospecimens. Results show that the detergent-free decellularization method preserved the esophageal matrix components and effectively removed cell nucleus. KYSE30 cancer cells proliferated well on and inside the DEM. KYSE30 cells cultured on the DEM in the dynamic bioreactor show different cancer marker expressions than those in the static well plate, and also share some similarities to the FFPE-ESCC biospecimens.
Show less - Date Issued
- 2023
- PURL
- http://purl.flvc.org/fau/fd/FA00014206
- Subject Headings
- Cancer, Esophageal Neoplasms, Biomimetics
- Format
- Document (PDF)